Curriculum Vitae

Ana Rita Pinto Seabra

Data da última atualização »Last update : 15/09/2015


Ana Rita Pinto Seabra. É Investigador Auxiliar no Instituto de Biologia Molecular e Celular. Publicou 11 artigos em revistas especializadas. Possui 29 itens de produção técnica. Actua na área de Ciências Biológicas Nas suas actividades profissionais interagiu com 67 colaboradores em co-autorias de trabalhos científicos.


Endereço de acesso a este CV:

http://www.degois.pt/visualizador/curriculum.jsp?key=3912282788327778


Dados pessoais (Personal data)
Nome completo
Full name
Ana Rita Pinto Seabra
Nome em citações bibliográficas
Quoting name
Seabra, Ana Rita
Categoria profissional
Position
Investigador Auxiliar
Domínio científico de atuação
Scientific domain
Ciências Naturais-Ciências Biológicas.
Ciências Agrárias-Outras Ciências Agrárias.
Ciências Médicas-Ciências da Saúde.
Endereço profissional
Professional address
Instituto de Biologia Molecular e Celular
Molecular and Celular Biology
Molecular Biology of Nitrogen Assimilation
Rua do Campo Alegre, 823
Porto
4150-180 Porto
Portugal
Telefone: (+351)226074900Extensão: 1616
Correio electrónico: ars@ibmc.up.pt
Homepage: https://www.ibmc.up.pt/research/research-groups/molecular-biology-nitrogen-assimilation
Sexo
Gender
Feminino»Female




Graus Académicos (Academic Degrees)
2007-2012 Doutoramento
Phd
Doutoramento em Biologia (5 anos » years) .
Faculdade de Ciências da Universidade do Porto, Portugal.

2000-2004 Licenciatura
Licentiate degree
Bioquímica - ramo bioquímica aplicada (4 anos » years) .
Faculdade de Ciências da Universidade do Porto, Portugal.





Formação complementar ( studies)
2013 Outros
Others
Project Management for Post-Docs.
Max-Planck-institut für Pflanzenzüchtungsforschung, Alemanha.

2011 Outros
Others
3rd UTEN training week - From the Lab to the market.
Universidade do Algarve, Portugal.





Vínculos profissionais (Professional Positions)
Instituto de Biologia Molecular e Celular
Fev/2015-Actual Investigador Auxiliar
Fev/2014-Dez/2014 Investigador Auxiliar
Mar/2012-Mai/2012 Investigador Auxiliar
Jul/2011-Fev/2012 Investigador Auxiliar
Jul/2007-Jun/2011 Outra Situação
Nov/2004-Jun/2007 Assistente de Investigação

Max-Planck-institut für Pflanzenzüchtungsforschung
Jun/2012-Jan/2014 Investigador Auxiliar

Universidade do Porto
Nov/2011-Abr/2012 Outra Situação

Instituto de Ciências Biomédicas Abel Salazar
Out/2003-Out/2004 Estagiário Investigador





Atividades de Investigação e Desenvolvimento (Research and Development activities)


Max-Planck-institut für Pflanzenzüchtungsforschung
Jun/2012-Jan/2014
Linhas de investigação»Research fields:


Plant-Obligate biotrophs interactions




Instituto de Biologia Molecular e Celular
Fev/2014-Dez/2014
Linhas de investigação»Research fields:


Post-translational regulation of Glutamine Synthetase (GS) by ubiquitin and/or ubiquitin-like proteins


Nitrogen signaling and plant development in Medicago truncatula


Jul/2008-Abr/2010
Linhas de investigação»Research fields:


Role and regulation of Glutamine synthetase (GS) in Medicago truncatula


Transcript expression profilling of root nodules with altered levels of GS activity






Estágios realizados (Traineeships)


Universidade do Porto
Nov/2011-Abr/2012
Estágios realizados»Traineeships:

   - UPINtech programme




Instituto de Ciências Biomédicas Abel Salazar
Out/2003-Out/2004
Estágios realizados»Traineeships:

   - Estudo de mecanismos imunológicos de resistência ou susceptibilidade à infecção experimental por Neospora caninum em murganhos.






Linhas de Investigação (Research fields)
1. Role and regulation of Glutamine synthetase (GS) in Medicago truncatula
Objectivos»Goals: (i) Characterize the entire GS gene family in Medicago truncatula (ii) Analyze patterns of gene and protein expression (iii) Determine the catalytic properties of each isoenzyme.
2. Transcript expression profilling of root nodules with altered levels of GS activity
Objectivos»Goals: (i) Transcriptome analysis of root nodules with inactive GS (ii) Identification of genes associated with GS activity .
3. Post-translational regulation of Glutamine Synthetase (GS) by ubiquitin and/or ubiquitin-like proteins
Objectivos»Goals: (i) Characterize the putative post-translational regulation of GS by ubiquitin and/or ubiquitin like proteins (ii) investigate the functional relevance of these modifications for nitrogen assimilation in Medicago truncatula.
4. Nitrogen signaling and plant development in Medicago truncatula
Objectivos»Goals: It is well known that microbes are able to modulate plant development. An important example is the interaction of legumes with nitrogen-fixing bacteria (Rhizobium), which leads to the development of a specialized organ where nitrogen fixation takes place – the root nodule. Nodule organogenesis is a process that occurs only under nitrogen-limiting conditions and that is promptly terminated when an inorganic source of nitrogen is sensed. Hence, components of nodulation pathways must surely overlap that of the nitrogen sensing/signaling pathways. Although some of the key players within nitrogen signaling pathways have been revealed in recent years, we are still far from completely understanding this complex network. I am currently interested in investigating how nitrogen signals are integrated with plant developmental processes, at the molecular level, particularly during the Legume-Rhizobium interaction. Within this context I aim to understand the role and regulation of prokaryotic GSI-like proteins in M. truncatula..
5. Plant-Obligate biotrophs interactions
Objectivos»Goals: A key question that has been poorly addressed is how plant obligate parasites adapt to new hosts? Recent findings lead us to hypothesize that heat shock protein 90 (HSP90) may be involved in such process. For instance, during stress HSP90 appears to link pre-acquired genetic diversity with fast appearance of new traits in eukaryotes. In addition, HSP90 appears to function as a virulence factor in human protozoa parasites. The goal of this research was to investigate the impact of cytoplasmic, and especially apoplastic HSP90s, on adaptation of obligate parasites to new plant hosts, to be specific, to investigate whether the proteins promote virulence and/or function as a buffer for genetic variation. To explore this topic I used the model plant Arabidopsis thaliana and the obligate parasitic oomycete Albugo laibachii. To reach my objective, I addressed the following questions: 1) How are HSP90s secreted? 2) What is the relative contribution of cytoplasmic versus apoplastic HSP90 to virulence? 3) What is the impact of stress, and hence the impact of HSPs to host shifts? .




Projetos de Investigação (Research projects)
Participação como Investigador
Participation as Researcher
2013-
Regulação da Glutamina sintetase pelo Oxido Nitrico em Medicago truncatula.-Regulation of Glutamine synthetase by Nitric Oxide in Medicago truncatula
Referência do projeto»Project reference: PTDC/BIA-PLA/2291/2012.


Participação como Doutorando
Participation as Phd student
2010-2013
Estudo da função e regulação da glutamina sintetase em sementes de Medicago truncatula-Study of the role and regulation of glutamine synthetase in Medicago truncatula seeds
Referência do projeto»Project reference: PTDC/AGR-AAM/099577/2008.


Participação como Desenvolvimento técnico
Participation as Technical developer
2005-2009
Determinação da estrutura tri-dimensional da glutamina sintetase de Medicago truncatula.-Elucidation of the three-dimensional structure of glutamine synthetase from the model legume Medicago truncatula
Referência do projeto»Project reference: POCI/AGR/61025/2004.

2004-2007
Estudo da contribuição da glutamina sintetase para a eficiência de assimilação do azoto e produtividade vegetal em M. truncatula-Analysis of the contribution of root nodule glutamine synthetase in controlling nitrogen assimilation efficiency and biomass production in M. truncatula
Referência do projeto»Project reference: POCTI/AGG/39079/2001.






Línguas (Languages)
Compreende
Understandig
Português (Bem), Inglês (Bem), Francês (Razoavelmente), Espanhol (Razoavelmente), Alemão (Razoavelmente).
Fala
Speaking
Português (Bem), Inglês (Bem), Espanhol (Razoavelmente).

Reading
Português (Bem), Inglês (Bem), Francês (Razoavelmente), Espanhol (Razoavelmente), Alemão (Razoavelmente).
Escreve
Writing
Português (Bem), Inglês (Bem).




Membro de Associações Profissionais/Científicas (Professional/Scientific Association membership)
Jan/2012 - Actual Sociedade Portuguesa de Bioquímica, Membro.




Produção científica, técnica e artística/cultural (Scientific, technical and artistical/cultural production)
Artigos em revistas com arbitragem científica
Papers in periodics with scientific refereeing
1. Seabra, Ana R; Carvalho, Helena G. 2015. "Glutamine synthetase in Medicago truncatula, unveiling new secrets of a very old enzyme", Frontiers in Plant Science 6, 578: 1 - 7.
2. Silva, Liliana S; Seabra, Ana R; Leitão, José N; Carvalho, Helena G. 2015. "Possible role of glutamine synthetase of the prokaryotic type (GSI-like) in nitrogen signaling in Medicago truncatula", Plant Science, 240: 98 - 108.
3. Torreira, Eva; Seabra, Ana R; Marriott, Hazel; Zhou, Min; Llorca, Óscar; Robinson, Carol V; Carvalho, Helena G; Fernández-Tornero, Carlos; Pereira, Pedro J. B. 2014. " The structures of cytosolic and plastid-located glutamine synthetases from Medicago truncatula reveal a common and dynamic architecture ", Acta Crystallographica Section D Biological Crystallography 70, 4: 981 - 993.
4. Seabra, Ana R; Silva, Liliana S; Carvalho, Helena G. 2013. "Novel aspects of glutamine synthetase (GS) regulation revealed by a detailed expression analysis of the entire GS gene family of Medicago truncatula under different physiological conditions", BMC Plant Biology 13, 1: 137 - 137.
5. Seabra, Ana R; Pereira, Patrícia A; Becker, Jörg D; Carvalho, Helena G. 2012. " Inhibition of Glutamine Synthetase by Phosphinothricin Leads to Transcriptome Reprograming in Root Nodules of Medicago truncatula ", Molecular Plant-Microbe Interactions 25, 7: 976 - 992.
6. Melo, P. M; Silva, L. S; Ribeiro, I.; Seabra, A. R; Carvalho, H. G. 2011. "Glutamine Synthetase Is a Molecular Target of Nitric Oxide in Root Nodules of Medicago truncatula and Is Regulated by Tyrosine Nitration", PLANT PHYSIOLOGY 157, 3: 1505 - 1517.
7. AR, Seabra; CP, Vieira; JV, Cullimore; HG, Carvalho. 2010. "Medicago truncatula contains a second gene encoding a plastid located glutamine synthetase exclusively expressed in developing seeds.", BMC plant biology 10, 1: 183 - 183.
8. Seabra, Ana R; Carvalho, Helena; Pereira, Pedro J. B. 2009. " Crystallization and preliminary crystallographic characterization of glutamine synthetase from Medicago truncatula ", Acta Crystallographica Section F Structural Biology and Crystallization Communications 65, 12: 1309 - 1312.
9. Lima, L.; Seabra, Ana; Melo, Paula; Cullimore, Julie; Carvalho, Helena. 2006. "Post-translational regulation of cytosolic glutamine synthetase of Medicago truncatula", Journal of Experimental Botany 57, 11: 2751 - 2761.
10. Lima, Lígia; Seabra, Ana; Melo, Paula; Cullimore, Julie; Carvalho, Helena. 2005. "Phosphorylation and subsequent interaction with 14-3-3 proteins regulate plastid glutamine synthetase in Medicago truncatula", Planta 223, 3: 558 - 567.
11. Teixeira, Luzia; Marques, Andreia; Meireles, Carla S; Seabra, Ana R; Rodrigues, Diana; Madureira, Pedro; Faustino, Augusto M. R; Silva, Carolina; Ribeiro, Adilia; Ferreira, Paula; Correia da C. J. M; Canada, Nuno; Vilanova, Manuel. 2005. "Characterization of the B-cell immune response elicited in BALB/c mice challenged with Neospora caninum tachyzoites", Immunology 116, 1: 38 - 52.



Apresentação oral de trabalho
Oral work presentation
1. LS, Silva; Seabra, Ana R. P; HG, Carvalho. Expression of GSI-like genes in root nodules of Medicago truncatula. ,10th European Nitrogen Fixation Conference,Munich,2012 (Poster).
2. Seabra, Ana R. P; PA, Pereira; JD, Becker; HG, Carvalho. Inhibition of glutamine synthetase by phosphinothricin leads to transcriptome reprogramming in Medicago truncatula root nodules. ,Meeting on Plant-Microbe Interactions,Braga,2011 (Comunicação).
3. Seabra, Ana R. P; PA, Pereira; JD, Becker; HG, Carvalho. Inhibition of glutamine synthetase by phosphinothricin leads to transcriptome reprogramming in Medicago truncatula root nodules.,Model Legume congress 2011,Sainte-Maxime,2011 (Poster).
4. PM, Melo; LS, Silva; Seabra, Ana R. P; HG, Carvalho. Regulation of glutamine synthetase by reactive nitrogen species in root nodules of Medicago truncatula.,10th International Conference on Reactive Oxygen and Nitrogen Species in Plants,Budapest,2011 (Comunicação).
5. PM, Melo; Seabra, Ana R. P; LS, Silva; HG, Carvalho. Reversible inactivation of glutamine synthetase by reactive nitrogen species in root nodules of Medicago truncatula., 9th European Nitrogen Fixation Conference,Geneve,2010 (Comunicação).
6. Seabra, Ana R. P; CP, Vieira; JV, Cullimore; HG, Carvalho. Characterization of a gene duplication encoding a plastid located glutamine synthetase exclusively expressed in developing seeds of Medicago truncatula. ,Vth International Congress on Legume Genetics and Genomics,Pacific grove,2010 (Comunicação).
7. PM, Melo; Seabra, Ana R. P; LS, Silva; HG, Carvalho. Regulation of glutamine synthetase by reactive nitrogen species in root nodules of Medicago truncatula.,XVII Congresso Nacional de Bioquímica,Porto,2010 (Poster).
8. PA, Pereira; Seabra, Ana R. P; N, Hohnjec; R, Balestrini; E, Limpens; P, Bonfante; H, Kuster; HG, Carvalho; JD, Becker. The Symbiotic Genetic Programme of the Model Legume Medicago truncatula., XXXV Portuguese Genetics Conference,Braga,2010 (Poster).
9. Seabra, Ana R. P; PA, Pereira; JD, Becker; HG, Carvalho. Inhibition of glutamine synthetase by phosphinothricin leads to transcriptome reprogramming in Medicago truncatula root nodules. ,XVII Congresso Nacional de Bioquímica,Porto,2010 (Poster).
10. Seabra, Ana R. P; PA, Pereira; JD, Becker; HG, Carvalho. Transcriptome analysis of Medicago truncatula root nodules following inhibition of glutamine synthetase by phosphinothricin,9th European Nitrogen Fixation Conference September,Geneve,2010 (Poster).
11. LS, Silva; Seabra, Ana R. P; PM, Melo; HG, Carvalho. Characterization of a glutamine synthetase phosphorylating kinase isolated from root nodules of Medicago truncatula.,9th European Nitrogen Fixation Conference September,Geneve,2010 (Poster).
12. LS, Silva; Seabra, Ana R. P; PM, Melo; HG, Carvalho. Identification of a glutamine synthetase putative phosphorylating kinase from Medicago truncatula.,XVII Congresso Nacional de Bioquímica,Porto,2010 (Poster).
13. I, Ribeiro; Seabra, Ana R. P; HG, Carvalho; PM, Melo. In vitro inactivation of plant glutamine synthetase by tyrosine nitration.,XVI Congress of the Federation of European Societies of Plant Biology (FESPB),Tampere,2008 (Poster).
14. Seabra, Ana R. P; C, Fernández-Tornero; G, Schoehn; PJ, Pereira; HG, Carvalho. Structural features of glutamine synthetase from Medicago truncatula.,XVI Congress of the Federation of European Societies of Plant Biology (FESPB),Tampere,2008 (Poster).
15. I, Ribeiro; PM, Melo; Seabra, Ana R. P; HG, Carvalho. In vitro inactivation of plant glutamine synthetase by tyrosine nitration.,16º Congresso Nacional de Bioquímica,Açores,2008 (Poster).
16. Seabra, Ana R. P; JV, Cullimore; HG, Carvalho. Overexpression of glutamine synthetase in root nodules of Medicago truncatula leads to enhanced nitrogen utilization efficiency.,XVI Congress of the Federation of European Societies of Plant Biology (FESPB),Tampere,2008 (Poster).
17. Seabra, Ana R. P; HG, Carvalho. Simultaneous 15N/13C labelling indicates that rhizobial nitrogen fixation is influenced by the levels of glutamine synthetase in root nodules.,16º Congresso Nacional de Bioquímica,Açores,2008 (Poster).
18. Seabra, Ana R. P; C, Fernández-Tornero; G, Schoehn; PJ, Pereira; HG, Carvalho. Decameric organization of glutamine synthetase from Medicago truncatula.,Nitrogen 2007-International Symposium on Inorganic Nitrogen Assimilation,Lancaster,2007 (Poster).
19. Seabra, Ana R. P; P, Thoquet; JV, Cullimore; HG, Carvalho. Assessment of the role of glutamine synthetase in the control of nitrogen metabolism in root nodules., 6th European Conference on Grain legume,Lisboa,2007 (Poster).
20. Seabra, Ana R. P; P, Thoquet; JV, Cullimore; HG, Carvalho. Analysis of transgenic Medicago truncatula overexpressing glutamine synthetase in root nodules: phenotypic characterization and transcript profiling.,Nitrogen 2007-International Symposium on Inorganic Nitrogen Assimilation,Lancaster,2007 (Poster).
21. Seabra, Ana R. P; HG, Carvalho; PJ, Pereira. Three-dimensional structure of an eukaryotic glutamine synthetase.,15º Congresso Nacional de Bioquímica,Aveiro,2006 (Poster).
22. Seabra, Ana R. P; HG, Carvalho. Silencing plastid located glutamine synthetase through RNAi in root nodules of transgenic Medicago truncatula.,15º Congresso Nacional de Bioquímica,Aveiro,2006 (Poster).
23. Teixeira, Luzia; Seabra, Ana R. P; Rodrigues, Diana; Ribeiro, Adilia; Vilanova, Manuel. Increased susceptibility to nosporosis in mice immunized with Neospora caninum tachyzoites structural antigens,XXX reunião anual da sociedade portuguesa de imunologia,Lisboa,2005 (Poster).
24. L, Lima; Seabra, Ana R. P; PM, Melo; HG, Carvalho. Identification of serine 97 as a major regulatory phosphorylation site in plastid glutamine synthetase from Medicago truncatula., IX Congresso Luso-Espanhol de Fisiologia Vegetal,Évora,2005 (Poster).
25. L, Lima; Seabra, Ana R. P; PM, Melo; HG, Carvalho. 14-3-3 binding controls proteolysis of plastid located glutamine synthetase in Medicago truncatula., IX Congresso Luso-Espanhol de Fisiologia Vegetal,Évora,2005 (Poster).
26. Teixeira, Luzia; Rodrigues, Diana; Seabra, Ana R. P; Ribeiro, Adilia; Vilanova, Manuel. Characterization of the B-cell immune response elicited in BALB/c mice challenged with Neospora caninum tachyzoites.,XXX reunião anual da sociedade portuguesa de imunologia,Lisboa,2005 (Poster).

Curso de curta duração lecionado
Taught short course
1. Seabra, Ana R. P; Carvalho, Helena. Highlights of plant engineering, 2014 (Extensão), promovido por II AEICBAS Biomedical Congress: an innovative multidisciplinary approach of life sciences.
Duração: 1 dias. Local: ICBAS, Cidade: Porto, Tipo de participação: Docente.
2. Seabra, Ana R. P; Figueiredo, Raquel. Porto de crianças - actividades de coadjuvação curricular, 2011 (Extensão), promovido por Município do Porto e IBMC.
Duração: 4 dias. Local: Escola básica da Pasteleira, Cidade: Porto, Tipo de participação: Docente.
3. Seabra, Ana R. P; Figueiredo, Raquel. Porto de crianças - Actividades de coadjuvação curricular, 2010 (Extensão), promovido por Município do Porto e IBMC.
Duração: 4 dias. Local: Escola básica Augusto Bessa, Cidade: Porto, Tipo de participação: Docente.



Outra produção artística/cultural
Other artistical/cultural production
1. Alexandre, Filipa; Barros, Ana; Seabra, Ana R. P; Nunes, Anabela; Silva, Andreia; Marinho, Joana; Correia, Maria R; Pereira, Marisa; Ferreira, Raquel; Moutinho, Tatiana. A origem das quase espécies - teatro de marionetas de mandrágora,2009.





Dados Complementares (Additional data)


Participação no júri de Graus Académicos
Academic Degrees jury participation


Bacharelato/Licenciatura
Degree of licentiate
1. Seabra, Ana Rita. Participação no júri de Francisca Isabel Alves da Silva Carvalhal. Caracterização molecular dos S2-, S5-, S10- e S25-haplótipos dos genes SFBB2, SFBB5, SFBB6, SFBB14 e SFBB16 em Malus, 2015. Trabalho de Conclusão de Curso (Bacharelato/Licenciatura em Bioquímica - ramo bioquímica aplicada) - Faculdade de Ciências da Universidade do Porto.
2. Seabra, Ana Rita Pinto. Participação no júri de Christina Casal Ribeiro Branco. Molecular characterization of the SFBB genes in Malus S2-, S5-, S10-, and S25-haplotypes, 2014. Trabalho de Conclusão de Curso (Bacharelato/Licenciatura em Licenciatura) - Faculdade de Ciências da Universidade do Porto.


Participação em eventos
Event participation
Participação como Membro da Comissão Científica
Participation as Member of the Program Committee
1. 1st “Triangle Meeting” between the Max Planck Institute for Plant Breeding Research in Cologne, the Sainsbury Laboratory University of Cambridge and the groups of Detlef Weigel and Gerd Jürgens from the Max Planck Institute for Developmental Biology in Tübingen. Cologne, Germany, 2013 (Encontro).
2. Careers in Science: Life after a PhD, 2010 (Simpósio).
Nome do evento: Careers in Science: Life after a PhD.








Indicadores de produção (Production indicators)

Total
Produção científica
Scientific production
11

Artigos científicos em revistas
Papers in periodics
11
Com arbitragem científica
With scientific refereeing
11

Total
Produção técnica
Technical production
29

Outros tipos de produção técnica
Other technical production
29

Total
Produção artística/cultural
Artistical/cultural production
1


Total
Dados complementares
(Additional data)
4

Participação no Júri de Graus Académicos
Academic Degrees jury participation
2
Participação em Eventos
Event participation
2


Outras informações relevantes
My research expertise relates mainly to nitrogen metabolism in legumes with emphasis on nitrogen assimilation in root nodules and developing seeds.
Most of my work has focus on clarifying aspects related to the role and regulation of glutamine synthetase isoenzymes in Medicago truncatula.
To accomplish my goals I have combined different disciplines such as physiology, molecular biology, biochemistry and structural biology and have successfully published my work in scientific journals of high impact.
During my research career I have also engaged on different projects related to host-microbe interactions, which have broadened my research skills in ecology, microbiology and immunology.
As a post-doc researcher I used the Arabidopsis thaliana-Albugo laibachii pathosystem to understand the life style of plant biotroph pathogens and as an undergraduate intern I studied the B-cell response of mice models to the parasite Neospora caninum.
Other interests include the development of sustainable and effective methods of crop production and actions of science communication for general public, which included participation as a teacher in children educational activities promoted by Porto Municipality.


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