Ana Paula Martins dos Reis Arez
|
|
Data da última atualização
»Last update
:
14/08/2013 |
Dados pessoais (Personal data)
Nome completo
Full name |
Ana Paula Martins dos Reis Arez |
Nome em citações bibliográficas
Quoting name |
Ana Paula Arez |
Categoria profissional
Position |
Investigador Auxiliar |
Domínio científico de atuação
Scientific domain |
Ciências Médicas-Ciências da Saúde. Ciências Naturais-Ciências Biológicas.
|
Sexo
Gender |
Feminino»Female |
Graus Académicos
(Academic Degrees)
2011 |
Agregação Aggregation |
Universidade Nova de Lisboa,
Portugal.
|
1996-2000 |
Doutoramento Phd |
Biologia (Biologia Molecular)
(4 anos » years)
.
Universidade de Lisboa,
Portugal.
|
1985-1992 |
Licenciatura Licentiate degree |
Biologia - Recursos Faunísticos e Ambiente
(5 anos » years)
.
Universidade de Lisboa,
Portugal.
|
Formação complementar ( studies)
2008-2008 |
Curso de curta duração Short course |
Gene expression across the erythrocytic life cycle of Plasmodium falciparum.
Universidad Complutense de Madrid,
Espanha.
|
2008-2008 |
Curso de curta duração Short course |
7th Course on Animal Experimentation.
Universidade Técnica de Lisboa,
Portugal.
|
2001-2007 |
Pós-Doutoramento Postdoctorate |
6 anos » years.
Universidade Nova de Lisboa,
Portugal.
|
2006-2006 |
Curso de curta duração Short course |
Pathogen Diversity and Disease Epidemiology.
Instituto Gulbenkian Ciência,
Portugal.
|
2002-2002 |
Curso de curta duração Short course |
Applied Malaria Bioinformatics (AMB02).
Instituto Gulbenkian Ciência,
Portugal.
|
2001-2001 |
Outros Others |
Training on culturing techniques of Plasmodium falciparum gametocytes. Istituto Superiore di Sanità,
Itália.
|
1994-1995 |
Outros Others |
Training on molecular biology techniques applied to malaria research. St Mary's Hospital Medical School,
Reino Unido.
|
1994-1994 |
Curso de curta duração Short course |
13rd European Course on Tropical Epidemiology.
Hospital Clínic de Barcelona,
Espanha.
|
1993-1993 |
Outros Others |
Training on basic techniques of ophidean venom characterisation. Liverpool School of Tropical Medicine,
Reino Unido.
|
Vínculos profissionais
(Professional Positions)
Universidade Nova de Lisboa |
Mar/2007-Actual |
Investigador Auxiliar |
Jan/2001-Fev/2007 |
Pos-Doutoramento |
Jan/1996-Nov/2000 |
Estudante doutoramento |
Jan/1993-Dez/1995 |
Estagiário Investigador |
Escola EB2+3 Luís de Camões |
Out/1993-Jul/1994 |
Docente |
Jan/1990-Dez/1993 |
Monitor |
Atividades de Investigação e Desenvolvimento (Research and Development activities)
Universidade Nova de Lisboa |
Atividades de Ensino (Teaching activities)
Universidade Nova de Lisboa |
Curso»Academic program: Biomedical Sciences Disciplinas lecionadas»Taught units:
- Molecular Epidemiology(Docente)
- Parasitology(Docente)
- Host-Parasite interactions(Docente)
- Member of Scientific Committee of PhD programme on Biomedical Sciences
|
Escola EB2+3 Luís de Camões |
Disciplinas lecionadas»Taught units:
- Ciências Naturais 5º ano (2º ciclo) + 8º ano (3º ciclo) (Docente)
- Ciências da Natureza 9º ano (3º ciclo)(Docente)
|
Curso»Academic program: Biologia - Recursos Faunísticos e Ambiente Disciplinas lecionadas»Taught units:
|
Atividades de Conselhos, Comissões e Consultorias (Councils, Comissions and Consultancy activities)
Universidade Nova de Lisboa |
Jan/2001-Actual |
|
Member of Scientific Council of CMDT, IHMT and Consultive Council of IHMT Library |
Outras atividades técnico científicas (Other technical-scientific activities)
Universidade Nova de Lisboa |
Jan/2001-Actual |
|
Atividade realizada»Activity: Advanced training - Supervision of PhD, MSc, BSc and other post-graduation projects |
Jan/2001-Actual |
|
Atividade realizada»Activity: Cooperation with CPLP countries |
Jan/1996-Nov/2000 |
|
Atividade realizada»Activity: PhD student, project "Molecular Epidemiology in malaria: transmission dynamics of Plasmodium mixed infections" |
Linhas de Investigação (Research fields)
1. |
Malaria parasite diversity - infection and transmission dynamics |
|
Objectivos»Goals:
Parasite epidemiology, especially malaria, transmission dynamics and interactions and ecological relationships between different
parasites co-infecting the same host have always been my main areas of interest . Within this scope, I have conducted several
research projects in malaria endemic areas, namely Guinea-Bissau, Cape Verde, Mozambique and Equatorial Guinea (on-going project).
Presently, main objectives are 1) To characterise circulating populations - Plasmodium species and genotypes, and describe
interactions between them and with both hosts (human and mosquitoes), 2) To analyse selective pressures acting upon drug resistance
genes and inferring on origin and spread of mutations through the comparison between flanking microsatellite sequences to
drug resistance associated genes and neutral loci, 3) To analyse host factors such as Duffy blood group and mosquito immune
related genes and to infer implications for malaria infection/transmission, 4) To investigate the parasite blood-stage dynamics
and what the clinical implications are when mixed infections occur through a new modelling approach and 5) To analyse the
effect of environmental conditions and transmission levels on the above aspects..
Domínio Científico:
Ciências Médicas / Área Científica:
Ciências da Saúde.
Domínio Científico:
Ciências Naturais / Área Científica:
Ciências Biológicas.
Objectivos socio-económicos:
Promoção geral dos conhecimentos; Protecção e promoção da saúde humana. Palavras-chave:
malaria; diversity; Plasmodium; Anopheles; transmission.
|
2. |
Host-Parasite interactions - factors of human host susceptibility/resistance to infection/disease |
|
Objectivos»Goals:
The interesting and unexpected observations obtained in Cape Verde led to a “new” line of research investigating which human
host factors may affect the susceptibility or resistance to malaria (infection and/or disease). Consequently, from approximately
2005 onwards, although continuing my former line of research, I have developed this new one, which is much more molecular/cellular
and experimentally-based. So far, encouraging results have been obtained and new plans and projects for future work are being
implemented.
Presently, we are focusing how disorders on the glycolytic and pentose phosphate pathways of the red blood cell may affect
Plasmodium infection, studying the effect of 3 enzymatic deficiencies (G6PD,PK,TPI) on parasite development. Main objectives
are: 1) To assess infection dynamics in enzyme-deficient and healthy blood both in in vitro as in vivo experimental models,
2) To analyse the proteomic profile of non-infected and infected erythrocytes (from cultures and rodent infections) as well
as of parasites (isolated from both deficient and normal host cells), 3) To determine frequencies of polymorphisms of g6pd,
pklr and tpi genes in African populations and 4) To investigate associations of individual haplotypes and malaria infection/
clinical status..
Domínio Científico:
Ciências Médicas / Área Científica:
Ciências da Saúde.
Domínio Científico:
Ciências Naturais / Área Científica:
Ciências Biológicas.
Objectivos socio-económicos:
Promoção geral dos conhecimentos; Protecção e promoção da saúde humana. Palavras-chave:
malaria; Host-parasite interactions; Human susceptibility/resistance; enzymophaties; glycolythic pathway.
|
Projetos de Investigação (Research projects)
Participação como Investigador responsável Participation as responsible Researcher |
2011- Alterações nas Vias Glicolítica e Pentoses-Fosfato do eritrócito - como afectam a infecção por Plasmódio?-Disorders on the Glycolytic and Pentose Phosphate Pathways of the Red Blood Cell - how do they affect Plasmodium infection? |
Referência do projeto»Project reference: PTDC/SAU-MET/110323/2009. Parceiro(s)»Partners:
Instituto Patologia e Imunologia Universidade Porto; Universidade de Coimbra; Centro Hospitalar de Coimbra; University of York. Financiador(es)»Funding:
Fundação para a Ciência e a Tecnologia.
|
2011- Factores que afectam a dinâmica de infecções concomitantes por Plasmodium numa área endémica: análise através de uma nova
abordagem de modelação-Factors affecting dynamics of Plasmodium concomitant infections in a malaria endemic area: analysis through a new modelling
approach
|
Referência do projeto»Project reference: PTDC/SAU-EPI/113326/2009. Parceiro(s)»Partners:
Instituto de Salud Carlos III. Financiador(es)»Funding:
Fundação para a Ciência e a Tecnologia.
|
2005-2009 Avaliação da epidemiologia da malária na República de Cabo Verde-Evaluation of epidemiology of malaria at Republic of Cabo Verde |
Referência do projeto»Project reference: POCI/SAU-ESP/55110/2004. Parceiro(s)»Partners:
Instituto Patologia e Imunologia Universidade Porto; Universidade de Coimbra. Financiador(es)»Funding:
Fundação para a Ciência e a Tecnologia.
|
2001-2003 Infecções mistas em malária: estudos sobre transmissão e interacção-Mixed infection in malaria: studies on transmission and interaction |
Referência do projeto»Project reference: POCTI/1999/ESP/35789/99. Parceiro(s)»Partners:
Center of Research in Health, Manhiça. Financiador(es)»Funding:
Fundação para a Ciência e a Tecnologia.
|
Participação como Investigador Participation as Researcher |
2010- Improvement of current and development of new vaccines for theileriosis and babesios of small ruminants (PiroVac) |
Referência do projeto»Project reference: FP7-KBBE-2009-3. Parceiro(s)»Partners:
Universidade Nova de Lisboa; Instituto de Biologia Experimental e Tecnológica; Forschungszentrum Borstel; University of Glasgow; Lanzhou Veterinary Research Institute; Bernhard Nocht Institut für Tropenmedizin; Institut National de la Sante et de la Recherche Medicale; Ministry of Agriculture and Rural Ministry of Agriculture and Rural Develo; Universitaet Bern; The Royal Veterinary College; Adnan Menderes University; Uzbek Veterinary Research Institute; University of Edinburgh; Parco Tecnologico Padano s.r. l.; Ecole Nationale Veterinaire de Nantes; Centro de Investigaciones en Ciencias Veterinarias y Agronomicas; Intervet/Schering-Plough Animal Health.
|
2009- Rede de Investigação e Desenvolvimento em Saúde/Malária da CPLP-Network of Research and Development in Health/Malaria from CPLP |
Referência do projeto»Project reference: RIDES - RIDES Malaria . Financiador(es)»Funding:
Instituto Português de Apoio ao Desenvolvimento.
|
2009- Plataforma Ibérica de Malária-Malaria Iberian Platform |
Referência do projeto»Project reference: PIM. Parceiro(s)»Partners:
Universidade de Lisboa; Instituto Patologia e Imunologia Universidade Porto; Universidad Complutense de Madrid; Instituto de Salud Carlos III; Centro de Investigación en Salud Internacional de Barcelona. Financiador(es)»Funding:
Fundação para a Ciência e a Tecnologia.
|
2008-2010 Evaluación, seguimiento y control de la malaria, en la Provincia de Misiones (Argentina). Capacitación del personal sanitario
en el manejo de brotes de malaria-Evaluation, monitoring and control of malaria in the Province of Misiones (Argentina). Training of health personnel in the
management of malaria outbreaks
|
Referência do projeto»Project reference: A/012190/07 (Spain). Parceiro(s)»Partners:
Universidade Nova de Lisboa; Universidad de Posadas. Financiador(es)»Funding:
Agência Espanhola de Cooperação Internacional e Desenvolvimento.
|
2005-2009 Estrutura populacional e fluxo genético dos parasitas da malária em ilhas-Population structure and genetic flux of malaria parasites in islands |
Referência do projeto»Project reference: POCTI/SAU-ESP/56903/2004. Financiador(es)»Funding:
Fundação para a Ciência e a Tecnologia.
|
2005-2008 Variabilidade genética de genes que expressam proteínas de reconhecimento (PRM) envolvidas na resposta imune do mosquito à
infecção por Plasmodium -Genetic variability of PRM-coding genes involved in mosquito immune response against Plasmodium
|
Referência do projeto»Project reference: POCTI/SAU-IMI/59489/2004. Financiador(es)»Funding:
Fundação para a Ciência e a Tecnologia.
|
2003-2006 Efeito da cloroquina na modulação da resposta do mosquito vector à infecção por Plasmodium-Effect of chloroquine in the modulation of the mosquito immune response against malaria infection |
Referência do projeto»Project reference: POCTI/MGI/344905/2002. Financiador(es)»Funding:
Fundação para a Ciência e a Tecnologia.
|
2004-2005 Scientific and technical follow-up of a pilot intervention of malaria control in the islands of São Tomé e Príncipe (STP),
using molecular and genetic approach
|
Referência do projeto»Project reference: US Navy/CMDT/CNE-STP/IPAD-Port. Parceiro(s)»Partners:
US Navy; Centro Nacional de Endemias. Financiador(es)»Funding:
Instituto Português de Apoio ao Desenvolvimento; US Navy.
|
1993-1994 Enzyme Linked Immunosorbent Assay (ELISA) for the quantification of envenoming by Viper in Portugal and evaluation of treatment
efficacy
|
Referência do projeto»Project reference: PBIC/SAU/1624/92. Parceiro(s)»Partners:
Liverpool School of Tropical Medicine. Financiador(es)»Funding:
Junta Nacional de Investigação Científica e Tecnológica.
|
Participação como Doutorando Participation as Phd student |
1997-1999 Malaria molecular epidemiology network |
Referência do projeto»Project reference: Praxis/2/2.2/1415/95. Parceiro(s)»Partners:
Karolinska Institutet; Institut Pasteur; London School of Hygiene and Tropical Medicine; Imperial College School of Medicine; University of Edinburgh; University of Oxford; Instituto de Salud Carlos III; Swiss Tropical Institute. Financiador(es)»Funding:
Fundação para a Ciência e a Tecnologia.
|
1994-1996 Network for field research on afrotropical malaria vectors |
Referência do projeto»Project reference: TS3-CT93-0236.
|
Participação como Desenvolvimento técnico Participation as Technical developer |
2007- Integrated Project to malaria control program in Chókwè Region (Gaza, Mozambique) |
Referência do projeto»Project reference: EC,SANTE-HEALTH/2006/105-398.
|
Línguas (Languages)
Compreende Understandig |
Inglês (Bem), Francês (Bem), Espanhol (Bem). |
Fala Speaking |
Inglês (Bem), Francês (Razoavelmente), Espanhol (Razoavelmente). |
Lê Reading |
Inglês (Bem), Francês (Bem), Espanhol (Bem). |
Escreve Writing |
Inglês (Bem), Francês (Razoavelmente), Espanhol (Pouco). |
Prémios e títulos (Awards Prizes, and Honours)
1993 |
Bolsa de Curta Duração para visita a uma instituição estrangeira,
Protocolo JNICT / BRITISH COUNCIL (Procº 423/RU).
|
1994 |
Bolsa de Investigação Científica,
Programa de Formação e Mobilidade de Recursos Humanos - JNICT (BIC 1049).
|
1996 |
Bolsa de Doutoramento,
Programa Praxis XXI/JNICT (PRAXIS XXI/BD/5813/95).
|
1997 |
Subsídio de apoio a participação em congressos, conferência e workshops,
Fundação Luso-Americana para o Desenvolvimento.
|
2000 |
Subsídio de apoio a participação em congressos internacionais, outras reuniões científicas e estágios muito curtos,
Fundação Calouste Gulbenkian.
|
2001 |
Bolsa de Pós Doutoramento,
Fundação para a Ciência e Tecnologia (SFRH/BPD/1624/2000).
|
2003 |
Bolsa de Curta Duração para estadia numa instituição estrangeira,
Fundação Calouste Gulbenkian (CD-AR, 418143-11-SET-2003).
|
Membro de Associações Profissionais/Científicas (Professional/Scientific Association membership)
Actual |
Ordem dos Biólogos, Membro.
|
Actual |
Sociedade Portuguesa de Herpetologia (SPH) , Membro fundador.
|
Actual |
Sociedade Portuguesa de Parasitologia (SPP), Membro.
|
Actual |
Sociedade Española de Medicina Tropical y Salud Internacional (SEMTSI), Membro.
|
Actual |
Associação dos Bolseiros de Investigação Científica (ABIC), Membro fundador.
|
Actual |
Associação Nacional dos Investigadores em Ciência e Tecnologia (ANICT), Membro.
|
Produção científica, técnica e artística/cultural
(Scientific, technical and artistical/cultural
production)
Capítulos de livros publicados Published book chapters |
1. |
Arez, Ana P; Nogueira, Fátima. 2012. Malária - deficiência em glucose-6-fosfato-desidrogenase e a terapêutica com primaquina. In Anais do Instituto de Higiene e Medicina Tropical, Edição Comemorativa, ed. P. Ferrinho, 55 - 56. . Lisboa: Universidade Nova de Lisboa.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
Artigos em revistas com arbitragem científica Papers in periodics with scientific refereeing |
1. |
Mendes, Cristina; Salgueiro, Patrícia; Gonzalez, Vicenta; Berzosa, Pedro; Benito, Agustin; do Rosário, V. E; de Sousa, B; Cano, Jorge; Arez, Ana P. 2013. "Genetic diversity and signatures of selection of drug resistance in Plasmodium populations from both human and mosquito hosts
in continental Equatorial Guinea", Malaria Journal 12, 1: 114 - 114.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
2. |
Machado, Patrícia; Manco, Licínio; Gomes, Cláudia; Mendes, Cristina; Fernandes, Natércia; Salomé, Graça; Sitoe, Luis; Chibute, Sérgio; Langa, José; Ribeiro, Letícia; Miranda, Juliana; Cano, Jorge; Pinto, João; Amorim, António; do Rosário, V. E; Arez, Ana P. 2012. "Pyruvate Kinase Deficiency in Sub-Saharan Africa: Identification of a Highly Frequent Missense Mutation (G829A;Glu277Lys)
and Association with Malaria", PLoS ONE 7, 10: e47071 - e47071.
|
|
|
|
3. |
Mendes, Cristina; Dias, Fernanda; Figueiredo, Joana; Mora, Vicenta G; Cano, Jorge; Sousa, Bruno; Rosário, Virgílio E.; Benito, Agustin; Berzosa, Pedro; Arez, Ana P. 2011. "Duffy negative antigen is no longer a barrier to Plasmodium vivax--molecular evidences from the African West Coast (Angola
and Equatorial Guinea)", PLoS Neglected Tropical Diseases 5, 6: 1192 - 1192. Recent reports of Plasmodium vivax infections, the most widely distributed species of human malaria, show that this parasite
is evolving and adapting, becoming not only more aggressive but also more frequent in countries where it was not present in
the past, becoming, therefore, a major source of concern. Thus, it is extremely important to perform new studies of its distribution
in West and Central Africa, where there are few reports of its presence, due to the high prevalence of Duffy-negative individuals.
The aim of this study was to investigate the presence of P. vivax in Angola and in Equatorial Guinea, using blood samples
and mosquitoes. The results showed that P. vivax seems to be able to invade erythrocytes using receptors other than Duffy,
and this new capacity is not exclusive to one strain of P. vivax, since we have found samples infected with two different
strains: VK247 and classic. Additionally we demonstrated that the parasite has a greater distribution than previously thought,
calling for a reevaluation of its worldwide distribution.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
4. |
Albuquerque, David; Manco, Licínio; Loua, Kovana M; Arez, Ana P; Trovoada, Maria J; Relvas, Luís; Millimono, Tamba S; Rath, Silvia L; Lopes, Dinora; Nogueira, Fátima; Varandas, Luís; Alvarez, Manuela; Ribeiro, Maria L. 2011. "SLC40A1 Q248H allele frequencies and associated SLC40A1 haplotypes in three West African population samples", Annals of human biology 38, 3: 378 - 381. Background: Ferroportin is a transmembrane protein responsible for iron export from enterocytes and macrophages. Mutation
c.744G ¿ T (Q248H), located in exon 6 of the ferroportin gene SLC40A1, is found as a polymorphism in populations of African
origin. This mutation has been extensively analysed in African-Americans, but poorly studied in native African populations.
Aim: To increase information about Q248H mutation frequency in native sub-Saharan populations examining three West African
populations.
Subjects and methods: Samples from S. Tomé e Príncipe (n = 115), Angola (n = 156) and Republic of Guinea (n = 170) were analysed
for Q248H mutation and for two polymorphisms, IVS1( - 24)G ¿ C and microsatellite (CGG)n, using standard molecular methodology.
Results: The estimated frequencies of Q248H allele were 2.2% in S. Tomé e Príncipe, 3.5% in Angola and 4.1% in Republic of
Guinea. Analysis of polymorphisms IVS1( - 24)G ¿ C and (CGG)n showed mutation allele c.744T to be strongly associated with
haplotype IVS1( - 24)G/(CGG)7.
Conclusions: This study confirmed the presence of Q248H mutation at polymorphic frequencies in three native sub-Saharan populations.
Analysis of two additional markers in the same gene support a single origin of the mutant allele c.744T in the haplotype background
IVS1( - 24)G/(CGG)7.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
5. |
Culleton, Richard; Coban, Cevayir; Zeyrek, Fadile Y; Cravo, Pedro; Kaneko, Akira; Randrianarivelojosia, Milijaona; Andrianaranjaka, Voahangy; Kano, Shigeyuki; Farnert, Anna; Arez, Ana P; Sharp, Paul M; Carter, Richard; Tanabe, Kazuyuki. 2011. "The Origins of African Plasmodium vivax; Insights from Mitochondrial Genome Sequencing", PLoS ONE 6, 12: 29137 - 29137.
|
|
|
|
6. |
Alves, Joana; Gomes, Bruno; Rodrigues, Regina; Silva, João; Arez, Ana P; Pinto, João; Sousa, Carla. 2010. "Mosquito fauna on the Cape Verde Islands (West Africa): an update on species distribution and a new finding", Journal of Vector Ecology 35, 2: 307 - 312. To evaluate the risk of transmission of vector-borne diseases, regular updates of the geographic distribution of insect vectors
are required. In the archipelago of Cape Verde, nine mosquito species have been reported. Of these, four are major vectors
of diseases that have been present in the archipelago: yellow fever, lymphatic filariasis, malaria and, currently, an outbreak
of dengue. In order to assess variation in mosquito biodiversity, we have carried out an update on the distribution of the
mosquito species in Cape Verde, based on an enquiry of 26 unpublished technical reports (1983–2006) and on the results of
an entomological survey carried out in 2007. Overall, there seems to be a general trend for an expansion of biological diversity
in the islands. Mosquito species richness was negatively correlated with the distance of the islands from the mainland but
not with the size of the islands. Human- and/or sporadic climatic-mediated events of dispersal may have contributed to a homogenization
of species richness regardless of island size but other ecological factors may also have affected the mosquito biogeography
in the archipelago. An additional species, Culex perexiguus, was collected for the first time in the archipelago during the
2007 survey.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
7. |
Almeida, Afonso; Rosário, Virgílio E.; Arez, Ana P; Cravo, Pedro. 2010. "Plasmodium vivax in the Democratic Republic of East Timor: Parasite prevalence and antifolate resistance-associated mutations", Acta Tropica 115, 3: 288 - 292. In the Democratic Republic of East Timor, Plasmodium falciparum and Plasmodium vivax malaria coexist, but limited information
is available about the latter species. Consequently, the prevalence of P. vivax and of its corresponding antifolate resistance-associated
mutations in the pvdhfr and pvdhps genes was assessed here. Blood samples were collected from 650 individuals distributed
among six districts, over two different periods, by either passive case detection (PCD) or active case detection (ACD). As
expected, malaria was over-represented in the PCD sample (26% PCD vs 5% ACD), because the infection increases medical care
seeking. Additionally, the relative frequency of P. vivax infections in symptomatic individuals (37%) was twice as high as
the one in the asymptomatic sampling group (18%), suggesting that that this parasite is accounting for a significant proportion
malaria-attributed morbidity. The frequency of specific sulfadoxine-pyrimethamine resistance-associated mutations genes was
ascertained in P. vivax positive samples by PCR-RFLP. Although no mutants were detected in codons 383 and 553 of pvdhps, 48%,
76% and 82% of P. vivax-infected samples harbored the dhfr 33L, 58R and 117N mutations, respectively. Additionally, the frequency
of parasites carrying both pvdhfr 58R and 117N mutant alleles accounted for a third of all genotypes analyzed, most likely
due to inadvertent SP use in the past. In conclusion, evidence-based information is provided to promote optimized drug deployment
and limit the evolution of resistance to antifolate resistance in P. vivax from East Timor.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
8. |
Almeida, Afonso; Rosário, Virgílio E.; Arez, Ana P; Cravo, Pedro. 2010. "Malaria epidemiology in the Democratic Republic of East Timor", Asian Pacific Journal of Tropical Medicine 3, 4: 283 - 287. Objective: To investigate malaria prevalence and socio-economic conditions in East Timor. Methods: Blood
samples were collected from 650 individuals distributed among six districts in East Timor. 434 and 216 individuals
were sampled by passive and active case detection, respectively (PCD, ACD). Results: The results showed that the
plasmodium infection prevalence was 18.9% with predominance of Plasmodium falciparum(P. falciparum) infections
(60.2%). The majority of cases were detected in children between 2-14 years old in both PCD and ACD. Participants
residing in Same and Lospalos were at a higher risk of malaria. The percentage of infections associated to the lack of
bed net use was twice as high as users (25.2% vs 12.5%). The prevalence of malaria among participants who lived
in brick/cement houses (8.3%) was two times less than those who lived in other types of houses (3.7%). There was a
significantly lower prevalence of infection detected in individuals living in complete housing constructions with absence
of domestic animals in or near the houses. Conclusions: The major risk factors.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
9. |
Sutherland, Colin J; Tanomsing, Naowarat; Nolder, Debbie; Oguike, Mary; Jennison, Charlie; Pukrittayakamee, Sasithon; Dolecek, Christiane; Hien, Tran T; Rosário, Virgílio E.; Arez, Ana P; Pinto, João; Michon, Pascal; Escalante, Ananias A; Nosten, Francois; Burke, Martina; Lee, Rogan; Blaze, Marie; Otto, Thomas D; Barnwell, John W; Pain, Arnab; Williams, John; White, Nicholas J; Day, Nicholas P. J; Snounou, Georges; Lockhart, Peter J; Chiodini, Peter L; Imwong, Mallika; Polley, Spencer D. 2010. "Two nonrecombining sympatric forms of the human malaria parasite Plasmodium ovale occur globally", The Journal of infectious diseases 201, 10: 1544 - 1550. Background. Malaria in humans is caused by apicomplexan parasites belonging to 5 species of the genus Plasmodium. Infections
with Plasmodium ovale are widely distributed but rarely investigated, and the resulting burden of disease is not known. Dimorphism
in defined genes has led to P. ovale parasites being divided into classic and variant types. We hypothesized that these dimorphs
represent distinct parasite species.
Methods. Multilocus sequence analysis of 6 genetic characters was carried out among 55 isolates from 12 African and 3 Asia-Pacific
countries.
Results. Each genetic character displayed complete dimorphism and segregated perfectly between the 2 types. Both types were
identified in samples from Ghana, Nigeria, São Tomé, Sierra Leone, and Uganda and have been described previously in Myanmar.
Splitting of the 2 lineages is estimated to have occurred between 1.0 and 3.5 million years ago in hominid hosts.
Conclusions. We propose that P. ovale comprises 2 nonrecombining species that are sympatric in Africa and Asia. We speculate
on possible scenarios that could have led to this speciation. Furthermore, the relatively high frequency of imported cases
of symptomatic P. ovale infection in the United Kingdom suggests that the morbidity caused by ovale malaria has been underestimated.
.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
10. |
Machado, Patrícia; Pereira, Rui; Rocha, Ana M; Manco, Licínio; Fernandes, Natércia; Miranda, Juliana; Ribeiro, Maria L; Rosário, Virgílio E.; Amorim, António; Gusmão, Leonor; Arez, Ana P. 2010. "Malaria: looking for selection signatures in the human PKLR gene region", British journal of haematology 149, 5: 775 - 784. The genetic component of susceptibility to malaria is both complex and multigenic and the better-known protective polymorphisms
are those involving erythrocyte-specific structural proteins and enzymes. In vivo and in vitro data have suggested that pyruvate
kinase deficiency, which causes a nonspherocytic haemolytic anaemia, could be protective against malaria severity in humans,
but this hypothesis remains to be tested. In the present study, we conducted a combined analysis of Short Tandem Repeats (STRs)
and Single Nucleotide Polymorphisms (SNPs) in the pyruvate kinase-encoding gene (PKLR) and adjacent regions (chromosome 1q21)
to look for malaria selective signatures in two sub-Saharan African populations from Angola and Mozambique, in several groups
with different malaria infection outcome. A European population from Portugal, including a control and a pyruvate kinase-deficient
group, was used for comparison. Data from STR and SNP loci spread along the PKLR gene region showed a considerably higher
differentiation between African and Portuguese populations than that usually found for neutral markers. In addition, a wider
region showing strong linkage disequilibrium was found in an uncomplicated malaria group, and a haplotype was found to be
associated with this clinical group. Altogether, this data suggests that malaria selective pressure is acting in this genomic
region.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
11. |
Alves, Joana; Machado, Patrícia; Silva, João; Gonçalves, Nilza; Ribeiro, Maria L; Faustino, Paula; Rosário, Virgílio E.; Manco, Licínio; Gusmão, Leonor; Amorim, António; Arez, Ana P. 2010. "Analysis of malaria associated genetic traits in Cabo Verde, a melting pot of European and sub Saharan settlers", Blood cells molecules & diseases 44, 1: 62 - 68. Malaria has occurred in the Cabo Verde archipelago with epidemic characteristics since its colonization. Nowadays, it occurs
in Santiago Island alone and though prophylaxis is not recommended by the World Health Organization, studies have highlight
the prospect of malaria becoming a serious public health problem as a result of the presence of antimalarial drug resistance
associated with mutations in the parasite populations and underscore the need for tighter surveillance.
Despite the presumptive weak immune status of the population, severe symptoms of malaria are not observed and many people
present a subclinical course of the disease. No data on the prevalence of sickle-cell trait and red cell glucose-6-phosphate
dehydrogenase deficiency (two classical genetic factors associated with resistance to severe malaria) were available for the
Cabo Verde archipelago and, therefore, we studied the low morbidity from malaria in relation to the particular genetic characteristics
of the human host population. We also included the analysis of the pyruvate kinase deficiency associated gene, reported as
putatively associated with resistance to the disease.
Allelic frequencies of the polymorphisms examined are closer to European than to African populations and no malaria selection
signatures were found. No association was found between the analyzed human factors and infection but one result is of high
interest: a linkage disequilibrium test revealed an association of distant loci in the PKLR gene and adjacent regions, only
in non-infected individuals. This could mean a more conserved gene region selected in association to protection against the
infection and/or the disease.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
12. |
Machado, Patrícia; Mendes, Cristina; Rosário, Virgílio E.; Arez, Ana P. 2010. "A contribuição dos polimorfismos humanos do eritrócito na protecção contra a malária", Revista Pan-Amazonica de Saude 1, 4: 85 - 96. A compreensão do complexo ciclo de vida da malária tem aumentado muito nos últimos anos mas, apesar de décadas de
pesquisa e luta contra a doença, esta continua a ser um dos principais problemas de saúde pública, especialmente nas
áreas mais pobres do planeta. Devido à sua elevada prevalência em certas regiões do globo, desde há cerca de 10 mil
anos, a malária tem exercido uma pressão seletiva muito forte no genoma humano. A componente genética de
suscetibilidade ao parasita é complexa, com uma variedade de polimorfismos a influenciar a patogénese e resposta do
hospedeiro, e um dos desafios na luta contra esta doença é avaliar estes determinantes de suscetibilidade e decifrar os
mecanismos envolvidos para utilizá-los como novos alvos para fármacos ou vacinas. Entre os polimorfismos genéticos
humanos descritos como protetores contra a malária, os mais comuns e melhor caracterizados envolvem proteínas
estruturais específicas (tais como as hemoglobinas S e C, as talassémias, o antigénio Duffy e o grupo sanguíneo O) e
enzimas eritrocitárias (como a deficiência de glucose-6-fosfato desidrogenase, e, mais recentemente descrita, a
deficiência de piruvato cinase). Esta pequena revisão aborda estas variantes genéticas e discute alguns dos resultados
controversos obtidos, assim como os mecanismos que podem justificar esta proteção.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
13. |
Almeida, Afonso; Arez, Ana P; Cravo, Pedro; Rosário, Virgílio E.. 2009. "Analysis of genetic mutations associated with anti-malarial drug resistance in Plasmodium falciparum from the Democratic Republic
of East Timor", Malaria Journal 8, -: 59 - 59. Background
In response to chloroquine (CQ) resistance, the policy for the first-line treatment of uncomplicated malaria in the Democratic
Republic of East Timor (DRET) was changed in early 2000. The combination of sulphadoxine-pyrimethamine (SP) was then introduced
for the treatment of uncomplicated falciparum malaria.
Methods
Blood samples were collected in two different periods (2003–2004 and 2004–2005) from individuals attending hospitals or clinics
in six districts of the DRET and checked for Plasmodium falciparum infection. 112 PCR-positive samples were inspected for
genetic polymorphisms in the pfcrt, pfmdr1, pfdhfr and pfdhps genes. Different alleles were interrogated for potential associations
that could be indicative of non-random linkage.
Results
Overall prevalence of mutations associated with resistance to CQ and SP was extremely high. The mutant form of Pfcrt (76T)
was found to be fixed even after five years of alleged CQ removal. There was a significant increase in the prevalence of the
pfdhps 437G mutation (X2 = 31.1; p = 0.001) from the first to second survey periods. A non-random association was observed
between pfdhfr51/pfdhps437 (p = 0.001) and pfdhfr 59/pfdhps 437 (p = 0.013) alleles.
Conclusion
Persistence of CQ-resistant mutants even after supposed drug withdrawal suggests one or all of the following: local P. falciparum
may still be inadvertently exposed to the drug, that mutant parasites are being "imported" into the country, and/or reduced
genetic diversity and low parasite transmission help maintain mutant haplotypes. The association between pfdhfr51/pfdhps437
and pfdhfr 59/pfdhps 437 alleles indicates that these are undergoing concomitant positive selection in the DRET. .
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
14. |
Manco, Licínio; Machado, Patrícia; Lopes, Dinora; Nogueira, Fátima; Rosário, Virgílio E.; Alonso, Pedro; Varandas, Luís; Trovoada, Maria J; Amorim, António; Arez, Ana P. 2009. "Analysis of TPI gene promoter variation in three sub-Saharan Africa population samples", American journal of human biology 21, -: 118 - 120. Population samples from Angola, Mozambique, and S. Tomé e Príncipe were screened for the TPI gene promoter variants -5A¿G,
-8G¿A and -24T¿G. Three haplotypes were identified in the three populations: the haplotype -5A-8G-24T (average frequency 65.3%)
and two less common haplotypes -5G-8G-24T (average frequency 24.7%) and -5G-8A-24T (average frequency 10.0%). A population
sample from Central Portugal showed the haplotype -5A-8G-24T in 139 chromosomes and one subject heterozygous for haplotype
-5G-8A-24G. The exact test of sample differentiation among three groups of malaria-infected individuals classified according
to the severity of the disease showed no significant differences. We confirmed TPI gene diversity in sub-Saharan Africa, but
we could not detect any association between TPI promoter variation and a malarial protective effect. Larger scale epidemiological
studies are thus required to clarify this putative mechanism of natural host defense against this worldwide public health
problem.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
15. |
Oliveira, E.; Salgueiro, Patrícia; Palsson, Katinka; Vicente, José L; Arez, Ana P; Jaenson, Thomas G; Caccone, A.; Pinto, João. 2008. "High Levels of Hybridization between Molecular Forms of Anopheles gambiae from Guinea Bissau", Journal of medical entomology 45, 6: 1057 - 1063. In the malaria vector Anopheles gambiae Giles sensu stricto, two molecular forms
denoted M and S are considered units of incipient speciation within this species. Very low hybrid
frequencies and signiÞcant genetic differentiation have been found in sympatric M- and S-form
populations. We studied the molecular form composition and the degree of genetic differentiation at
15 microsatellites in two samples of An. gambiae collected in two consecutive years from Bissau, Guinea
Bissau. High frequencies of M/S hybrids (19Ð24%) were found in this area. Coincidently, very low
levels of genetic differentiation were detected between forms when analysis involved microsatellites
mapped at chromosome-3 (mean Fst, 0.000Ð0.002). The single exception was the X-linked AGXH678,
for which high differentiation was measured (Fst, 0.158Ð0.301). This locus maps near the centromere
of chromosome X, a low recombination region in which selection is likely to promote divergence
between M and S forms. These results strongly suggest that the degree of isolation between M and
S forms, considered the units of incipient speciation within An. gambiae, is not homogenous throughout
the species distribution range. |
|
|
|
16. |
Hassan, D. A; Arez, A. P; Mohamed, H. S; Elhussein, A. M; Ibrahim, M. E; Abdulhadi, N. H. 2008. "The reduced sequestration of <I>Plasmodium-falciparum</I>-infected erythrocytes among malaria cases with sickle-cell trait:
in-vivo evidence from Sudan", Annals of Tropical Medicine and Parasitology 102, 8: 743 - 748.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
17. |
Arez, A. P; do Rosrio, V. E. 2008. "The Relevance of Molecular Markers in the Analysis of Malaria Parasite Populations", Transboundary and Emerging Diseases 55, 5-6: 226 - 232. Malaria is one of the main human public health problems in the tropical world and is possibly becoming an emerging disease
too in regions where it has been controlled. It has been an excellent model in the area of molecular studies, with scientific
validation of techniques, application of data mainly in studies of parasite diversity and information on a number of different
aspects associated with infection and disease. The transfer of the gathered knowledge and experience in malaria to other infections
is of great use and we briefly review a number of molecular markers, methodologies and techniques mostly used for Plasmodium
detection, as well as identification or characterization of parasite populations. Selection of appropriate techniques depends
on the questions raised and the studies’ objectives – the antigen-coding genes, microsatellite loci and drug-resistance associated
markers being the three most analysed classes of markers. The need of validation and standardization of laboratory protocols
is addressed and discussed as it may determine the comparison of data between different studies and laboratories, with relevance
in field-collected samples or studies.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
18. |
Tanomsing, Naowarat; Imwong, Mallika; Pukrittayakamee, Sasithon; Chotivanich, K.; Looareesuwan, S.; Mayxay, M.; Dolecek, Christiane; Hien, Tran T; Rosário, Virgílio E.; Arez, Ana P; Michon, Pascal; Snounou, Georges; White, Nicholas J; Day, Nicholas P. J. 2008. "Genetic analysis of the dihydrofolate reductase-thymidylate synthase gene from geographically diverse isolates of Plasmodium
malariae", American Journal of Tropical Medicine and Hygiene 79, 6: 188 - 188. |
|
|
|
19. |
Tanomsing, N.; Imwong, M.; Pukrittayakamee, S.; Chotivanich, K.; Looareesuwan, S.; Mayxay, M.; Dolecek, C.; Hien, T. T; do Rosario, V. E; Arez, A. P; Michon, P.; Snounou, G.; White, N. J; Day, N. P. J. 2007. "Genetic Analysis of the Dihydrofolate Reductase-Thymidylate Synthase Gene from Geographically Diverse Isolates of Plasmodium
malariae", Antimicrobial Agents and Chemotherapy 51, 10: 3523 - 3530. Plasmodium malariae, the parasite responsible for quartan malaria, is transmitted in most areas of malaria endemicity and
is associated with significant morbidity. The sequence of the gene coding for the enzyme dihydrofolate reductase-thymidylate
synthase (DHFR-TS) was obtained from field isolates of P. malariae and from the closely related simian parasite Plasmodium
brasilianum. The two sequences were nearly 100% homologous, adding weight to the notion that they represent genetically distinct
lines of the same species. A survey of polymorphisms of the dhfr sequences in 35 isolates of P. malariae collected from five
countries in Asia and Africa revealed a low number of nonsynonymous mutations in five codons. In five of the isolates collected
from southeast Asia, a nonsynonymous mutation was found at one of the three positions known to be associated with antifolate
resistance in other Plasmodium species. Five isolates with the wild-type DHFR could be assayed for drug susceptibility in
vitro and were found to be sensitive to pyrimethamine (mean 50% inhibitory concentration, 2.24 ng/ml [95% confidence interval,
0.4 to 3.1]).
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
20. |
Vicente, José L; Salgueiro, Patrícia; Arez, Ana P; Cravo, Pedro; Ferreira, Conceição; Rosário, Virgílio E.; Pinto, João. 2007. "Genetic characterisation of Plasmodium falciparum populations in São Tomé and Príncipe islands, West Africa.", Tropical Medicine and International Health 12, suppl 1: 78 - 78. |
|
|
|
21. |
Mendes, Cristina; Dias, Fernanda; Rosário, Virgílio E.; Varandas, Luís; Arez, Ana P. 2007. "Prevalence of Plasmodium sp. infection in children in the Kuanza Sul province, Angola.", Tropical Medicine and International Health 12, Suppl 1: 166 - 167. |
|
|
|
22. |
Alves, Joana; Machado, Patrícia; Gonçalves, Nilza; Silva, João; Gusmão, Leonor; Alves, Cíntia; Manco, Licínio; Rosário, Virgílio E.; Amorim, António; Arez, Ana P. 2007. "Molecular epidemiology: some host genetics factors related to malaria in Santiago – Cabo Verde Islands.", Tropical Medicine and International Health 12, Suppl 1: 167 - 167. |
|
|
|
23. |
Lopes, Luís F; Zamora, F.; Rocha, L.; Marques, Patrícia; Mendes, Cristina; Martinez-Barnetche, J.; Leandro, M.J.; Arevalo, M.; Herrera, Socrates; Rosário, Virgílio E.; Arez, Ana P; Silveira, Henrique. 2007. "Anopheles albimanus immune response to single and mixed infections with Plasmodium falciparum and Plasmodium vivax. ", Tropical Medicine and International Health 12, Suppl 1: 201 - 202. |
|
|
|
24. |
Miranda, Juliana; Gonçalves, Nilza; Picanço, Isabel; Rosário, Virgílio E.; Faustino, Paula; Arez, Ana P. 2007. "Sickle-cell trait and red cell glucose-6-phosphate dehydrogenase status and malaria morbidity in Angola.", Tropical Medicine and International Health 12, Suppl 1: 238 - 238. |
|
|
|
25. |
Alves, Joana; Roque, Ana; Cravo, Pedro; Valdez, Tomás; Jelinek, Tomas; do Rosário, V. E; Arez, Ana. 2006. "Epidemiological characterization of Plasmodium falciparum in the Republic of Cabo Verde: implications for potential large-scale
re-emergence of malaria", Malaria Journal 5, 1: 32 - 32. Abstract
Background
Malaria has come near eradication at archipelago of Cabo Verde in 1970. Infections are now only observed in Santiago, where
outbreaks occur. In these islands, malaria is considered by the international community as being of limited risk and, therefore,
no prophylaxis is recommended. Since the understanding of factors that determine malaria outbreaks are crucial for controlling
the disease, the present study aimed to investigate if the malaria infections observed in Santiago Island are maintained in
isolated foci and in asymptomatic individuals.
Methods
The occurrence of asymptomatic carriers in villages with history of malaria as well as the level of exposure of these populations
were investigated using PCR and serological analyses.
Results
Results indicate that malaria is maintained as asymptomatic and sub-patent infections and that the majority of the circulating
parasite populations harbour chloroquine-resistant mutations.
Conclusion
These observations highlight the alarming prospect of malaria to become a serious public health problem and underscore the
need for a tighter surveillance. .
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
26. |
Marques, Patrícia; Saúte, Francisco; Pinto, Vera V; Cardoso, Sónia; Pinto, João; Alonso, Pedro; Rosário, Virgílio E.; Arez, Ana P. 2005. "Plasmodium species mixed infections in two areas of Manhiça District, Mozambique", International journal of biological sciences 1, na: 96 - 102. We compared the distribution patterns of individual Plasmodium species and mixed-species infections in two geographically
close endemic areas, but showing environmental differences. Comparisons concerned circulating Plasmodium infections in both
human and mosquito vector populations in the dry and wet seasons, at a micro-epidemiological level (households). Both areas
revealed a very high overall prevalence of infection, all year-round and in all age groups. Plasmodium falciparum was the
predominant species, being found in the vast majority of infected individuals regardless of the presence of other species.
Plasmodium malariae and Plasmodium ovale occurred almost exclusively in mixed infections. Seasonal variation in P. malariae
prevalence was observed in one area but not in the other. A decrease in P. malariae prevalence concurred with a marked increase
of P. falciparum prevalence. However this was strongly dependent on age and when analysing infections at the individual level,
a different pattern between co-infecting species was unveiled. Regarding transmission patterns, in both areas, P. falciparum
gametocytes predominated in single infections regardless of age and P. malariae gametocyte carriage increased when its overall
prevalence decreased.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
27. |
Drakeley, C.; Schellenberg, D.; Kihonda, J.; Sousa, C. A; Arez, A. P; Lopes, D.; Lines, J.; Mshinda, H.; Lengeler, C.; Schellenberg, J. A; Tanner, M.; Alonso, P.. 2003. "An estimation of the entomological inoculation rate for Ifakara: a semi-urban area in a region of intense malaria transmission
in Tanzania", Tropical Medicine and International Health 8, 9: 767 - 774. Summary An entomological study on vectors of malaria and their relative contribution to Plasmodium falciparum transmission
in the semi-urban area of Ifakara, south-eastern Tanzania, was conducted. A total of 32 houses were randomly sampled from
the area and light trap catches (LTC) performed in one room in each house every 2 weeks for 1 year. A total of 147 448 mosquitoes
were caught from 789 LTC; 26 134 Anopheles gambiae s.l., 615 A. funestus, 718 other anophelines and 119 981 culicines. More
than 60% of the total A. gambiae s.l. were found in five (0.6%) LTCs, with a maximum of 5889 caught in a single trap. Of 505
A. gambiae s.l. speciated by polymerase chain reaction, 91.5% were found to be A. arabiensis. Plasmodium falciparum sporozoite
enzyme-linked immunosorbent assay tests were performed on 10 108 anopheles mosquitoes and 39 (0.38%) were positive. Entomological
inoculation rate (EIR) estimates were generated using a standard method and an alternative method that allows the calculation
of confidence intervals based on a negative binomial distribution of sporozoite positive mosquitoes. Overall EIR estimates
were similar; 31 vs. 29 [95% confidence interval (CI): 19, 44] infectious bites per annum, respectively. The EIR ranged from
4 (95% CI: 1, 17) in the cool season to 108 (95% CI: 69, 170) in the wet season and from 54 (95% CI: 30, 97) in the east of
the town to 15 (95% CI: 8, 30) in the town centre. These estimates show large variations over short distances in time and
space. They are all markedly lower than those reported from nearby rural areas and for other parts of Tanzania.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
28. |
Gil, J; Nogueira, Fátima; Strömberg-Nörklit, J.; Carrolo, Margarida; Casimiro, Catarina; Lopes, Dinora; Arez, A. P; Cravo, Pedro; Rosário, Virgílio E.. 2003. "Detection of atovaquone and Malarone™ resistance conferring mutations in Plasmodium falciparum cytochrome b gene (cytb)", Molecular and Cellular Probes 17, 2-3: 85 - 89. Clinical treatment failures of the hydroxynaphthoquinone atovaquone or its combination with proguanil (Malarone™) in Plasmodium
falciparum malaria has been recently documented. These events have been associated to single nucleotide polymorphisms (SNPs)
in the parasite cytochrome b gene (cytb). In this report we describe a set of nest PCR-RFLP methods developed for the fast
detection of all known cytb mutations associated to resistance to these drugs. The methods were successfully applied for the
analysis of phenol–chloroform extracted DNA samples from patients not cured by Malarone™, and from an established parasite
clone. Further, the protocol for the detection of the A803C mutation was applied to 164 DNA field samples extracted through
crude methanol-based protocols, originated from several malaria settings.
The PCR-RFLP methods here presented can be used as a valuable for the clinical detection and study of Malarone™ and atovaquone
P. falciparum resistance.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
29. |
Arez, Ana P; Pinto, João; Palsson, Katinka; Snounou, Georges; Jaenson, Thomas G; Rosário, Virgílio E.. 2003. "TRANSMISSION OF MIXED PLASMODIUM SPECIES AND PLASMODIUM FALCIPARUM GENOTYPES ", The American journal of tropical medicine and hygiene 68, 2: 161 - 168. We studied malaria transmission by comparing parasite populations in humans and mosquito vectors at the household level. Blood
samples were collected from all inhabitants for microscopic detection of gametocytes and polymerase chain reaction analysis.
The next morning, blood-fed resting mosquitoes were collected inside the bed nets used by the individuals surveyed the previous
afternoon. After 8 days of maintenance, mosquitoes were dissected, and midguts and salivary glands were recovered for polymerase
chain reaction analysis. Results showed that parasite distribution was the same in the 2 hosts when compared at each household
but was different when whole populations were analyzed. Different associations of Plasmodium species seem to occur in humans
(Plasmodium falciparum/Plasmodium malariae) and mosquitoes (P. falciparum/Plasmodium ovale). Regarding P. falciparum infections,
a higher proportion of single-genotype infections and less allele diversity are observed in mosquitoes than in humans. .
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
30. |
Meyer, Christian G; May, Jurgen; Arez, Ana P; Gil, Jose P; do Rosario, V. 2002. "Review: Genetic diversity of Plasmodium falciparum: asexual stages", Tropical Medicine and International Health 7, 5: 395 - 408.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
31. |
Fernandes, Natércia; Silveira, Henrique; Franco, Sofia; Arez, Ana P; Forte, J.M.; Rosário, Virgílio E.. 2001. "Detection of malaria parasites in paraffin-embedded spleen and placental tissues by nested PCR", Transactions of the Royal Society of Tropical Medicine and Hygiene 95, 3: 293 - 294. |
|
|
|
32. |
Färnert, Anna; Arez, Ana P; Babiker, Hamza A; Beck, Hans-Peter; Benito, Agustin; Björkman, Anders; Bruce, Marian C; Conway, David J; Day, Karen; Henning, L.; Mercereau-Puijalon, Odille; Ranford-Cartwright, Lisa; Rubio, J. M; Snounou, Georges; Walliker, David; Zwetyenga, Joana; Rosário, Virgílio E.. 2001. "Genotyping of Plasmodium falciparum infections by PCR: a comparative multicentre study", Transactions of the Royal Society of Tropical Medicine and Hygiene 95, 2: 225 - 232. Genetic diversity of malaria parasites represents a major issue in understanding several aspects of malaria infection and
disease. Genotyping of Plasmodium falciparum infections with polymerase chain reaction (PCR)-based methods has therefore been
introduced in epidemiological studies. Polymorphic regions of the msp1, msp2 and glurp genes are the most frequently used
markers for genotyping, but methods may differ. A multicentre study was therefore conducted to evaluate the comparability
of results from different laboratories when the same samples were analysed. Analyses of laboratory-cloned lines revealed high
specificity but varying sensitivity. Detection of low-density clones was hampered in multiclonal infections. Analyses of isolates
from Tanzania and Papua New Guinea revealed similar positivity rates with the same allelic types identified. The number of
alleles detected per isolate, however, varied systematically between the laboratories especially at high parasite densities.
When the analyses were repeated within the laboratories, high agreement was found in getting positive or negative results
but with a random variation in the number of alleles detected. The msp2 locus appeared to be the most informative single marker
for analyses of multiplicity of infection. Genotyping by PCR is a powerful tool for studies on genetic diversity of P. falciparum
but this study has revealed limitations in comparing results on multiplicity of infection derived from different laboratories
and emphasizes the need for highly standardized laboratory protocols. |
|
|
|
33. |
Arez, A; Lopes, Dinora; Pinto, João; Franco, Sofia; Snounou, Georges; Rosário, Virgílio E.. 2000. "Plasmodium sp.: Optimal Protocols for PCR Detection of Low Parasite Numbers from Mosquito (Anopheles sp.) Samples", Experimental Parasitology 94, 4: 269 - 272.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
|
|
|
34. |
Arez, Ana P; Snounou, Georges; Pinto, João; Sousa, Carla; Modiano, David; Ribeiro, Henrique; Franco, Sofia; Alves, Joana; Rosário, Virgílio E.. 1999. "A clonal Plasmodium falciparum population in an isolated outbreak of malaria in the Republic of Cabo Verde", Parasitology 118, Pt 4: 347 - 355. We present the first parasitological, molecular and longitudinal analysis of an isolated outbreak of malaria. This outbreak
occurred on Santiago Island (Republic of Cabo Verde), a region where malaria is hypoendemic and controlled, and thus the population
is considered non-immune. Blood samples were collected from the inhabitants over 1 month and during cross-sectional surveys
in the following year. The presence and nature of the parasites was determined by PCR. Plasmodium falciparum was the only
species detected. Genetic analysis revealed that the circulating parasites were genetically homogeneous, and probably clonal.
Gametocytes were found throughout this period. Our data suggest that this represented a focal outbreak, resulting in the infection
of at least 40% of the villagers with a clonal parasite line. Thus, P. falciparum infections can persist for at least 1 year
in a substantial proportion (10%) of the hosts. Implications for malaria control and the interpretation of epidemiological
data are discussed. |
|
|
|
35. |
Färnert, Anna; Arez, Ana P; Correia, Ana T; Björkman, Anders; Snounou, Georges; Rosário, Virgílio E.. 1999. "Sampling and storage of blood and the detection of malaria parasites by polymerase chain reaction", Transactions of the Royal Society of Tropical Medicine and Hygiene 93, 1: 50 - 53. Polymerase chain reaction (PCR) is now widely used in malaria research for analysis of field samples. However, little has
been reported regarding loss of sensitivity due to field methodology. Therefore, studies were carried out in relation to blood
sampling (anticoagulants, culture medium, filter paper), storage (temperature, time and immediate lysis) and handling (repeated
thawing and freezing). The PCR was unaffected by citrate and EDTA but partly inhibited by heparin (inhibition was reversed
by heparinase at optimal concentrations). Samples collected on filter paper showed a significant 100-fold lower sensitivity
(compared to control samples frozen immediately after collection) when stored at 30 degrees C and 60% humidity; and the paper
quality appeared to be critical. Storage of unprocessed whole blood at 4 degrees C, 20 degrees C or 30 degrees C rarely resulted
in any loss of sensitivity. Repeated thawing generally resulted in 10-fold loss of sensitivity compared to blood kept frozen
until DNA extraction. The presence of antimalarial drug did not apparently affect sensitivity. We conclude that the mode of
collection and storage of blood samples may influence the sensitivity of detection of malaria parasites by PCR. This may be
critical in studies including individuals with low parasitaemia, mixed infections and comparison of data from different settings. |
|
|
|
36. |
Pinto, João; Sousa, Carla; Arez, Ana P; Alves, Joana; Modiano, David; Petrarca, Vincenzo; Charlwood, Jacques D; Rosário, Virgílio E.. 1999. "Assessment of malaria transmission in an area with very low mosquito density", Research and reviews in parasitology 59, na: 23 - 26. |
|
|
|
37. |
Arez, Ana P; Palsson, Katinka; Pinto, João; Franco, Sofia; Dinis, João; Jaenson, Thomas G; Snounou, Georges; Rosário, Virgílio E.. 1997. "Transmission of mixed malaria species and strains by mosquitoes, as detected by PCR, in a study area in Guinea-Bissau", Parassitologia 39, 1: 65 - 70. Parasites present in blood samples of asymptomatic carriers and in the midgut of mosquitoes collected within a few days from
the same households, have been analysed by PCR. A high prevalence (32%) of infected mosquitoes was observed and, in half of
these, two parasite species were found simultaneously. The distribution of parasite species in the mosquito correlated with
that found in the infected persons. Genotype patterns of Plasmodium falciparum populations were however found to be different
in the two sets of samples. These results and the potential of PCR are discussed with reference to investigations of the dynamics
of malaria transmission. |
|
|
|
38. |
Pinto, João; Arez, Ana P; Franco, Sofia; Rosário, Virgílio E.; Palsson, Katinka; Jaenson, Thomas G; Snounou, Georges. 1997. "Simplified methodology for PCR investigation of midguts from mosquitoes of the Anopheles gambiae complex, in which the vector
and Plasmodium species can both be identified", Annals of tropical medicine and parasitology 91, 2: 217 - 219. |
|
|
|
39. |
Arez, Ana P; Laing, C. G; Brito, José C; Rosário, Virgílio E.; Theakston, R. D. G. 1993. "Preliminary studies on the characterisation of venom from Vipera latastei latastei (Boscá, 1878) (Reptilia, Viperidae) collected
in NW Portugal", Portugaliae Zoologica 2, na: 37 - 42. |
|
|
|
Trabalhos completos/resumidos em eventos com arbitragem científica Papers in conference proceedings with scientific refereeing |
1. |
Alves, Joana; Sousa, Carla; Gomes, Bruno; Rodrigues, Regina; Silva, João; Arez, Ana P; Pinto, João. 2009. "Actualização da fauna culicidológica de Cabo Verde", Trabalho apresentado em XI Congresso Ibérico de Parasitologia, In Acta Parasitológica Portuguesa, Lisboa. |
|
|
|
2. |
Mendes, Cristina; Dias, Fernanda; Rosário, Virgílio E.; Varandas, Luís; Arez, Ana P. 2007. "Prevalence of Plasmodium sp. infection in children in the Kuanza Sul province, Angola", Trabalho apresentado em 5th European Congress on Tropical Medicine and International Health, In Proceedings of the 5th European Congress on Tropical Medicine and International Health, Amsterdam. |
|
|
|
3. |
Miranda, Juliana; Gonçalves, Nilza; Picanço, Isabel; Rosário, Virgílio E.; Faustino, Paula; Arez, Ana P. 2007. "Sickle-cell trait and red cell glucose-6-phosphate dehydrogenase status and malaria morbidity in Angola", Trabalho apresentado em 5th European Congress on Tropical Medicine and International Health, In Proceedings of the 5th European Congress on Tropical Medicine and International Health, Amsterdam. |
|
|
|
4. |
Miranda, Juliana; Gonçalves, Nilza; Picanço, Isabel; Rosário, Virgílio E.; Faustino, Paula; Arez, Ana P. 2006. "Avaliação da drepanocitose como determinante genético da resistência à malária em crianças com diferentes níveis de gravidade
da doença numa unidade hospitalar em Luanda, Angola", Trabalho apresentado em X Congresso Português de Parasitologia, In Acta Parasitológica Portuguesa, Lisboa. |
|
|
|
5. |
Almeida, Afonso; Arez, Ana P; Cravo, Pedro. 2006. "Estudos epidemiológicos e resistência aos anti-maláricos em Timor Leste", Trabalho apresentado em X Congresso Português de Parasitologia, In Acta Parasitológica Portuguesa, Lisboa. |
|
|
|
6. |
Machado, Patrícia; Alves, Joana; Gusmão, Leonor; Alves, Cíntia; Rosário, Virgílio E.; Amorim, António; Arez, Ana P. 2006. "Malária e deficiência em piruvato quinase em Cabo Verde", Trabalho apresentado em X Congresso Português de Parasitologia, In Acta Parasitológica Portuguesa, Lisbon. |
|
|
|
7. |
Lopes, Luís F; Leandro, M.J.; Zamora, F.; Rocha, L.; Arevalo, M.; Herrera, Socrates; Rosário, Virgílio E.; Arez, Ana P; Silveira, Henrique. 2006. "Resposta imune do mosquito Anopheles albimanus a infecções simples e mistas por Plasmodium falciparum e Plasmodium vivax", Trabalho apresentado em X Congresso Português de Parasitologia, In Acta Parasitológica Portuguesa, Lisbon. |
|
|
|
8. |
Fernandes, Natércia; Arez, Ana P; Rosário, Virgílio E.. 2006. "Malária na Gravidez: Diversidade Genética do Plasmodium falciparum na Placenta e Sangue Periférico de Mulheres Grávidas.", Trabalho apresentado em XLII Congresso da Sociedade Brasileira de Medicina Tropical, In Revista da Sociedade Brasileira de Medicina Tropical, Piauí. |
|
|
|
9. |
Lopes, Luís F; Marques, Patrícia; Mendes, Cristina; Leandro, M.J.; Zamora, F.; Rocha, L.; Arevalo, M.; Herrera, Socrates; Rosário, Virgílio E.; Silveira, Henrique; Arez, Ana P. 2006. "Infecções Mistas com Plasmodium falciparum e Plasmodium vivax em Mosquitos Anopheles albimanus: Dinâmica da Transmissão e
Resposta Imune", Trabalho apresentado em XLII Congresso da Sociedade Brasileira de Medicina Tropical, In Revista da Sociedade Brasileira de Medicina Tropical, Piauí. |
|
|
|
Textos em jornais ou revistas Texts in newspapers or magazines |
1. |
Arez, Ana P; Lima, Sónia C; Rosário, Virgílio E.. 01 jan. 2003. "Novas Doenças em Portugal" Ambiente 21, 60 - 61. |
Apresentação oral de trabalho Oral work presentation |
1. |
Arez, Ana P. Enzimopatias eritrocitárias e efeitos na infeção malárica,Jornadas de Parasitologia III,Porto,2013 (Conferência ou palestra). |
2. |
Arez, Ana P. Enzimopatias eritrocitárias e efeitos na infeção malárica,Porto,2013 (Conferência ou palestra). |
3. |
Arez, Ana P. Avaliação da epidemiologia da malária na República de Cabo Verde,Workshop em Biologia e Medicina Tropicais,Oeiras,2012 (Conferência ou palestra). |
4. |
Machado, Patrícia; Manco, Licínio; Gomes, Cláudia; Mendes, Cristina; Fernandes, Natércia; Salomé, Graça; Chibute, Sérgio; Ribeiro, Letícia; Miranda, Juliana; Cano, Jorge; Pinto, João; Gusmão, Leonor; Amorim, António; Rosário, Virgílio E.; Arez, Ana P. Pyruvate kinase deficiency and malaria protection in humans: A population genetics study.,MEEGID XI Conference,New Orleans,2012 (Congresso). |
5. |
Arez, Ana P. Malária - factores do hospedeiro de susceptibilidade/resistência à infecção/doença,Lisboa,2010 (Seminário). |
6. |
Arez, Ana P. Pesquisas em malária - do campo para a bancada,Lisboa,2010 (Seminário). |
7. |
Arez, Ana P. Aspectos imunológicos da malária - implicações para o diagnóstico,Lisbon,2010 (Seminário). |
8. |
Machado, Patrícia; Pereira, Rui; Rocha, Ana M; Manco, Licínio; Fernandes, Natércia; Miranda, Juliana; Ribeiro, Maria L; Rosário, Virgílio E.; Amorim, António; Gusmão, Leonor; Arez, Ana P. Malaria: a selection pressure in the PKLR human gene?,Anthropology and Health: trends for the next decade,Coimbra,2010 (Comunicação). |
9. |
Salgueiro, Patrícia; Vicente, José L; Ferreira, Conceição; Arez, Ana P; Rosário, Virgílio E.; Pinto, João. Genetic diversity and population structure of Plasmodium falciparum in the West African archipelago of São Tomé and Príncipe:
a nine years survey,MEEGID X Conference,Amsterdam,2010 (Comunicação). |
10. |
Albuquerque, David; Relvas, Luís; Trovoada, Maria J; Alvarez, Manuela; Arez, Ana P; Ribeiro, Maria L; Manco, Licínio. Analysis of SLC40A1 gene diversity in African and Portuguese population samples.,Anthropology and Health: trends for the next decade. ,Coimbra,2010 (Poster). |
11. |
Mendes, Cristina; Mora, Vicenta G; Figueiredo, Joana; Cano, Jorge; Sousa, Bruno; Rosário, Virgílio E.; Benito, Agustin; Silveira, Henrique; Berzosa, Pedro; Arez, Ana P. Mainland Equatorial Guinea: Plasmodium diversity and interaction with both hosts.,Wellcome Trust Scientific Conferences: Genomic Epidemiology of Malaria.,Cambridge,2010 (Poster). |
12. |
Machado, Patrícia; Pereira, Rui; Rocha, Ana M; Manco, Licínio; Fernandes, Natércia; Miranda, Juliana; Ribeiro, Maria L; Rosário, Virgílio E.; Amorim, António; Gusmão, Leonor; Arez, Ana P. Searching for selection signatures imprinted by malaria in the human PKLR gene region,Wellcome Trust Scientific Conferences: Genomic Epidemiology of Malaria.,Cambridge,2010 (Poster). |
13. |
Mendes, Cristina; Mora, Vicenta G; Cano, Jorge; Sousa, Bruno; Silveira, Henrique; Pinto, João; Rosário, Virgílio E.; Benito, Agustin; Berzosa, Pedro; Arez, Ana P. Diversidade populacional e dinâmica de transmissão de Plasmodium sp. e Plasmodium falciparum na Guiné-Equatorial.,XLVI Congresso da Sociedade Brasileira de Medicina Tropical,Paraná,2010 (Poster). |
14. |
Pereira, Rui; Rocha, Ana M; Manco, Licínio; Fernandes, Natércia; Miranda, Juliana; Ribeiro, Maria L; Rosário, Virgílio E.; Amorim, António; Gusmão, Leonor; Arez, Ana P. Malária: identificação de marcas de selecção na região do gene PKLR em humanos.,XLVI Congresso da Sociedade Brasileira de Medicina Tropical,Paraná,2010 (Poster). |
15. |
Machado, Patrícia; Pereira, Rui; Rocha, Ana M; Manco, Licínio; Fernandes, Natércia; Miranda, Juliana; Ribeiro, Maria L; Rosário, Virgílio E.; Amorim, António; Gusmão, Leonor; Arez, Ana P. Malaria: looking for selection signatures in the human PKLR gene region,2nd Meeting of Malaria Iberian Platform,Madrid,2009 (Comunicação). |
16. |
Mendes, Cristina; Mora, Vicenta G; Lúcio, A.; Cano, Jorge; Sousa, Bruno; Silveira, Henrique; Pinto, João; Rosário, Virgílio E.; Benito, Agustin; Berzosa, Pedro; Arez, Ana P. Population diversity and transmission dynamics of Plasmodium sp. and Plasmodium falciparum in Equatorial Guinea,2nd Meeting of Malaria Iberian Platform,Madrid,2009 (Comunicação). |
17. |
Alves, Joana; Sousa, Carla; Gomes, Bruno; Rodrigues, Regina; Silva, João; Arez, Ana P; Pinto, João. Actualização da fauna culicidológica de Cabo Verde,XI Congresso Ibérico de Parasitologia,Lisboa,2009 (Comunicação). |
18. |
Manco, Licínio; Machado, Patrícia; Lopes, Dinora; Nogueira, Fátima; Rosário, Virgílio E.; Varandas, Luís; Arez, Ana P; Ribeiro, Maria L. Prevalence of G6PD-deficient alleles in a population sample from Angola.,12ª Reunião da Sociedade Portuguesa de Genética Humana,Lisboa,2008 (Poster). |
19. |
Arez, Ana P. Molecular Epidemiology in malaria - validation and standardization of molecular markers in parasite identification,EU-FP6 INCO-DEV INCOME Workshop,Carcavelos,2007 (Seminário). |
20. |
Vicente, José L; Salgueiro, Patrícia; Arez, Ana P; Cravo, Pedro; Ferreira, Conceição; Rosário, Virgílio E.; Pinto, João. Genetic characterisation of Plasmodium falciparum populations in São Tomé and Príncipe islands, West Africa,5th European Congress on Tropical Medicine and International Health” (ECTMIH 07),Amsterdam,2007 (Comunicação). |
21. |
Alves, Joana; Machado, Patrícia; Gonçalves, Nilza; Silva, João; Gusmão, Leonor; Alves, Cíntia; Manco, Licínio; Rosário, Virgílio E.; Amorim, António; Arez, Ana P. Molecular epidemiology: some host genetics factors related to malaria in Santiago – Cabo Verde Islands.,5th European Congress on Tropical Medicine and International Health (ECTMIH 07),Amesterdam,2007 (Poster). |
22. |
Miranda, Juliana; Gonçalves, Nilza; Picanço, Isabel; Rosário, Virgílio E.; Faustino, Paula; Arez, Ana P. Sickle-cell trait and red cell glucose-6-phosphate dehydrogenase status and malaria morbidity in Angola.,5th European Congress on Tropical Medicine and International Health” (ECTMIH 07),Amsterdam,2007 (Poster). |
23. |
Mendes, Cristina; Dias, Fernanda; Rosário, Virgílio E.; Varandas, Luís; Arez, Ana P. Prevalence of Plasmodium sp. infection in children in the Kuanza Sul province, Angola,2007 (Poster). |
24. |
Lopes, Luís F; Zamora, F.; Rocha, L.; Marques, Patrícia; Mendes, Cristina; Martinez-Barnetche, J.; Leandro, M.J.; Arevalo, M.; Herrera, Socrates; Rosário, Virgílio E.; Arez, Ana P; Silveira, Henrique. Anopheles albimanus immune response to single and mixed infections with Plasmodium falciparum and Plasmodium vivax,5th European Congress on Tropical Medicine and International Health” (ECTMIH 07),Amsterdam,2007 (Poster). |
25. |
Alves, Joana; Machado, Patrícia; Gonçalves, Nilza; Silva, João; Gusmão, Leonor; Alves, Cíntia; Manco, Licínio; Rosário, Virgílio E.; Amorim, António; Arez, Ana P. Malaria epidemiology and human host genetics in Santiago – Cape Verde Islands.,10th edition Portugaliæ Genetica,Porto,2007 (Poster). |
26. |
Miranda, Juliana; Gonçalves, Nilza; Picanço, Isabel; Rosário, Virgílio E.; Faustino, Paula; Arez, Ana P. Estudo de determinantes genéticos de susceptibilidade/resistência à malária em crianças com diferentes níveis de gravidade
da doença numa unidade hospitalar em Luanda, Angola.,XLIII Congresso da Sociedade Brasileira de Medicina Tropical,Campos do Jordão,2007 (Poster). |
27. |
Machado, Patrícia; Alves, Joana; Gusmão, Leonor; Alves, Cíntia; Manco, Licínio; Rosário, Virgílio E.; Amorim, António; Arez, Ana P. Malária e deficiência de piruvato cinase.,XLIII Congresso da Sociedade Brasileira de Medicina Tropical,Campos do Jordão,2007 (Poster). |
28. |
Félix, Rute; Sousa, M.; Pinto, João; Arez, Ana P; Rosário, Virgílio E.; Silveira, Henrique. Variabilidade genética das Proteínas de Reconhecimento de Peptidoglicanos em mosquitos vectores da malária.,XLIII Congresso da Sociedade Brasileira de Medicina Tropical,Campos do Jordão,2007 (Comunicação). |
29. |
Miranda, Juliana; Gonçalves, Nilza; Picanço, Isabel; Rosário, Virgílio E.; Faustino, Paula; Arez, Ana P. Avaliação da drepanocitose como determinante genético da resistência à malária em crianças com diferentes níveis de gravidade
da doença numa unidade hospitalar em Luanda, Angola,X Congresso Português de Parasitologia,Lisboa,2006 (Comunicação). |
30. |
Almeida, Afonso; Arez, Ana P; Cravo, Pedro. Estudos epidemiológicos e resistência aos anti-maláricos em Timor Leste,X Congresso Português de Parasitologia,Lisboa,2006 (Comunicação). |
31. |
Alves, Joana; Roque, Ana; Cravo, Pedro; Valdez, Tomás; Jelinek, Tomas; Rosário, Virgílio E.; Arez, Ana P. Epidemiological characterization of Plasmodium falciparum in the Republic of Cabo Verde: implications for potential large-scale
re-emergence of malaria,XLII Congresso da Sociedade Brasileira de Medicina Tropical,Piauí,2006 (Poster). |
32. |
Lopes, Luís F; Marques, Patrícia; Mendes, Cristina; Leandro, M.J.; Zamora, F.; Rocha, L.; Arevalo, M.; Herrera, Socrates; Rosário, Virgílio E.; Silveira, Henrique; Arez, Ana P. Single and mixed Plasmodium sp. infections in Anopheles mosquitoes: transmission dynamics and immune response,Vivax Malaria Research: 2005 and Beyond,Rockville MD,2005 (Poster). |
33. |
Lopes, Luís F; Marques, Patrícia; Mendes, Cristina; Leandro, M.J.; Zamora, F.; Rocha, L.; Arevalo, M.; Herrera, Socrates; Rosário, Virgílio E.; Silveira, Henrique; Arez, Ana P. Single and mixed Plasmodium sp. infections in Anopheles mosquitoes: transmission dynamics and immune response.,Cost Action 857, 2nd Annual Workshop,Beatenberg,2005 (Poster). |
34. |
Marques, Patrícia; Saúte, Francisco; Pinto, Vera V; Cardoso, Sónia; Alonso, Pedro; Rosário, Virgílio E.; Arez, Ana P. Plasmodium species mixed infections in ecologically different areas,Cost Action 857, 1st Annual Workshop,Lisboa,2004 (Comunicação). |
35. |
Rosário, Virgílio E.; Alves, Joana; Pinto, João; Cravo, Pedro; Arez, Ana P. General considerations on data interpretation from field studies on parasite diversity,Cost Action 857, 1st Annual Workshop,Lisboa,2004 (Comunicação). |
36. |
Arez, Ana P. Mixed infections and molecular epidemiology,3rd AMVTN Workshop on Molecular Biology and Immunology of Malaria Vaccines,Maputo,2002 (Seminário). |
37. |
Arez, Ana P; Marques, Patrícia; Saúte, Francisco; Alonso, Pedro; Rosário, Virgílio E.. Plasmodium species mixed infections in two ecologically different areas of Manhiça district, Mozambique.,3rd European Congress on Tropical Medicine and International Health,Lisbon,2002 (Poster). |
38. |
Arez, Ana P. Os custos sócio-económicos da malária,III Encontro de Educação Ambiental,Monte da Caparica,2000 (Conferência ou palestra). |
39. |
Arez, Ana P; Pinto, João; Silveira, Henrique; Palsson, Katinka; Snounou, Georges; Jaenson, Thomas G; Rosário, Virgílio E.. Transmission of mixed Plasmodium infections,Oxford 2000. New Challenges in Tropical Medicine and Parasitology,Oxford,2000 (Comunicação). |
40. |
Arez, Ana P; Fernandes, Natércia; Silveira, Henrique; Rosário, Virgílio E.. Plasmodium falciparum genotypes in pregnant women peripheral blood and placenta samples.,Oxford 2000. New Challenges in Tropical Medicine and Parasitology,Oxford,2000 (Poster). |
41. |
Arez, Ana P; Babiker, Hamza A; Beck, Hans-Peter; Benito, Agustin; Björkman, Anders; Bruce, Marian C; Conway, David J; Day, Karen; Färnert, Anna; Mercereau-Puijalon, Odille; Ranford-Cartwright, Lisa; Rosário, Virgílio E.; Rubio, J. M; Snounou, Georges; Walliker, David; Zwetyenga, Joana. Genotyping of Plasmodium falciparum infections: a comparative multicenter study.,11th BSP Malaria Meeting,London,1999 (Comunicação). |
42. |
Arez, Ana P; Färnert, Anna; Correia, Ana T; Björkman, Anders; Snounou, Georges; Rosário, Virgílio E.. Sampling and storage of blood and the detection of malaria parasites by polymerase chain reaction.,MIM (Multilateral Initiative on Malaria) African Malaria Conference.,Durban,1999 (Poster). |
43. |
Arez, Ana P; Färnert, Anna; Correia, Ana T; Björkman, Anders; Snounou, Georges; Rosário, Virgílio E.. Sampling and storage of blood and the detection of malaria parasites by polymerase chain reaction.,10th BSP (British Society for Parasitology) Malaria Meeting,Edinburgh,1998 (Poster). |
44. |
Lopes, Dinora; Arez, Ana P; Pinto, João; Alves, Ana C; Casaca, Teresa; Snounou, Georges; Rosário, Virgílio E.. Evaluation of inhibition level of polymerase chain reaction (PCR) for the detection of Plasmodium sp. in Anopheles sp. mosquitoes.,10th BSP (British Society for Parasitology) Malaria Meeting. ,Edinburgh,1998 (Poster). |
45. |
Arez, Ana P; Färnert, Anna; Correia, Ana T; Björkman, Anders; Snounou, Georges; Rosário, Virgílio E.. Sampling and storage of blood and the detection of malaria parasites by polymerase chain reaction.,2nd European Congress on Tropical Medicine. Tropical Health for the 21st century. ,Liverpool,1998 (Poster). |
46. |
Lopes, Dinora; Arez, Ana P; Pinto, João; Alves, Ana C; Casaca, Teresa; Snounou, Georges; Rosário, Virgílio E.. Evaluation of inhibition level of polymerase chain reaction (PCR) for the detection of Plasmodium sp. in Anopheles sp. mosquitoes.,2nd European Congress on Tropical Medicine. Tropical Health for the 21st century,Liverpool,1998 (Poster). |
47. |
Arez, Ana P. Epidemia clonal de malária na região de Cabo Verde (95/96) ,Seminários de Imunologia Clínica,Lisboa,1997 (Seminário). |
48. |
Arez, Ana P. Identification of Plasmodia in anopheline mosquitoes,Workshop on PCR-based detection and identification of pathogens in tropical ticks,Utrecht,1997 (Seminário). |
49. |
Arez, Ana P; Alves, Joana; Pinto, João; Sousa, Carla; Franco, Sofia; Snounou, Georges; Rosário, Virgílio E.. Longitudinal Study of an Outbreak of “Clonal” Plasmodium falciparum in the Republic of Cabo Verde.,46th Annual Meeting of American Society of Tropical Medicine and Hygiene. ,Orlando,1997 (Poster). |
50. |
Arez, Ana P; Alves, Joana; Pinto, João; Sousa, Carla; Franco, Sofia; Rosário, Virgílio E.. Estudo do surto epidémico de malária em Santiago, na R. de Cabo Verde, em Outubro de 1995.,III Reunião Anual da Sociedade Portuguesa de Parasitologia. ,Coimbra,1996 (Comunicação). |
51. |
Pinto, João; Arez, Ana P; Franco, Sofia; Snounou, Georges; Nabion, Marcelino; Rosário, Virgílio E.. Simplificação da metodologia para identificação por PCR, de plasmódios e vectores do complexo Anopheles gambiae.,III Reunião Anual da Sociedade Portuguesa de Parasitologia.,Coimbra,1996 (Comunicação). |
52. |
Arez, Ana P; Snounou, Georges; Alves, Joana; Rosário, Virgílio E.. An epidemic in a non-endemic area caused by a homogeneous Plasmodium falciparum parasite population.,8th Malaria Meeting of British Society for Parasitology,Glasgow,1996 (Comunicação). |
53. |
Arez, Ana P; Alves, Joana; Franco, Sofia; Pinto, João; Sousa, Carla; Snounou, Georges; Rosário, Virgílio E.. Parasite Biodiversity and an epidemics of malaria in Santiago, Republic of Cabo Verde.,I Jornadas Luso-Caboverdeanas de Doenças Infecciosas,Cidade da Praia,1996 (Comunicação). |
54. |
Rosário, Virgílio E.; Arez, Ana P. Biodiversity of malaria parasites,I Congresso Latino Americano de Malária. ,Quito,1996 (Comunicação). |
55. |
Arez, Ana P; Pinto, João; Palsson, Katinka; Dinis, João; Jaenson, Thomas G; Rosário, Virgílio E.. Identification of malaria parasites in mosquitoes, by PCR, from a study area in Guinea Bissau.,XX International Congress of Entomology,Florence,1996 (Poster). |
56. |
Arez, Ana P; Laing, C. G; Rosário, Virgílio E.; Theakston, R. D. G. Preliminary studies on the characterization of venom from Vipera latastei latastei collected at Northwest of Portugal.,XI Meeting of IST,Arezzo,1993 (Poster). |
57. |
Arez, Ana P; Caetano, Maria H. Determinação da estrutura etária, longevidade, idade de maturação sexual e crescimento em três populações portuguesas de Rana
perezi (SEOANE, 1885).,II Congreso Luso-Español y VI Congreso Español de Herpetologia,Granada,1992 (Comunicação). |
58. |
Arez, Ana P; Caetano, Maria H. Détermination de l'âge chez Rana perezi (SEOANE, 1885) au moyen de la squelettochronologie.,21ème Congrès de la Société Herpetologique de France. ,Sigean,1992 (Comunicação). |
Desenvolvimento de material didáctico ou pedagógico Didactic and pedagogic material development |
1. |
Arez, Ana P. Relatório de curso avançado não conferente de grau - Protozoologia Molecular,2011. Curso que integra a oferta pedagógica do Instituto de Higiene e Medicina Tropical de Lisboa. |
Organização de evento Event organization |
1. |
Arez, Ana P; Pinto, João. Workshop on Population genetics in malaria: parasites, vectors and humans,2008 (Oficina / Outra). |
Dados Complementares (Additional data)
Tese de Doutoramento Phd Thesis |
1. |
Patrícia Machado, Pyruvate kinase and glucose-6-phosphate dehydrogenase deficiencies and their association with malaria – population genetics
and proteomic studies, 2013. Tese (PhD in Biomedical Sciences) - Universidade Nova de Lisboa, Bolseiro(a) de Fundação para a Ciência e a Tecnologia (Orientador). |
2. |
Fernanda Dias, The effect of host and parasite factors in susceptibility to malaria severity, 2012. Tese (Tropical Medicine) - Universidade Nova de Lisboa (Co-orientador). |
3. |
Joana Alves, Epidemiologia da malária em Santiago, Cabo Verde – factores genéticos humanos e estrutura populacional do mosquito vector, 2011. Tese (PhD in Biomedical Sciences) - Universidade Nova de Lisboa, Bolseiro(a) de Fundação para a Ciência e a Tecnologia (Orientador). |
4. |
Afonso de Almeida, Epidemiological studies and resistance to antimalarials in East Timor, 2009. Tese (PhD in Biomedical Sciences) - Universidade Nova de Lisboa, Bolseiro(a) de Fundação Calouste Gulbenkian (Co-orientador). |
1. |
Tiago Vaz, Erythrocyte enzymopathies - efects on malaria infection, 2012. Tese (Human Genetics and Infectious Diseases) - Universidade Nova de Lisboa, Bolseiro(a) de Fundação para a Ciência e a Tecnologia (Orientador). |
2. |
Cristina Mendes, Diversidade Populacional e Dinâmica de Transmissão de Plasmodium sp. e Plasmodium falciparum na Guiné-Equatorial, 2008. Tese (PhD in Biomedical Sciences) - Universidade Nova de Lisboa, Bolseiro(a) de Fundação para a Ciência e a Tecnologia (Orientador). |
Dissertação de Mestrado Master degree dissertation |
1. |
Mónica Guerra, Pesquisa de assinaturas de selecção de malária na região do gene humano tpi (triose fosfato isomerase), 2012. Dissertação (MSc in Medical Parasitology) - Universidade Nova de Lisboa (Orientador). |
2. |
Ana Margarida Rosa, Pyruvate kinase deficiency protection against malaria: in vitro evaluation of 2,3-diphosphoglycerate toxicity and Plasmodium
falciparum susceptibility, 2011. Dissertação (Human Biology and Environment) - Universidade de Lisboa (Orientador). |
3. |
Juliana Miranda, Estudo de determinantes genéticos de susceptibilidade/resistência em crianças com diferentes níveis clínicos de gravidade
de malária (Luanda, Angola), 2007. Dissertação (MSc in Medical Parasitology) - Universidade Nova de Lisboa (Co-orientador). |
4. |
Patrícia Marques, Estudo das infecções simples e mistas por Plasmodium sp. na população humana em duas áreas distintas do Distrito da Manhiça,
Moçambique, 2004. Dissertação (MSc in Medical Parasitology) - Universidade Nova de Lisboa (Co-orientador). |
5. |
Joana Alves, Determinação de focos residuais de P. falciparum em Santiago, Cabo Verde, 2004. Dissertação (MSc in Medical Parasitology) - Universidade Nova de Lisboa (Co-orientador). |
Monografia de conclusão de curso de aperfeiçoamento/especialização Specialization course conclusion monograph |
1. |
Tiago Vieira, Contribution for the project: studies of transmission dynamics of Plasmodium species mixed infections in Manhiça (Mozambique), 2003. Monografia (Aperfeiçoamento/Especialização em BSc in Chemical Sciences and Environment) - Instituto Piaget (Orientador). Part-time training in the discipline of “Research Project I” of BSC in Chemical Sciences and Environment, Instituto Piaget.
Classification 18/20. |
Trabalho de conclusão de curso de Bacharelato/Licenciatura Bachelor/Licenciate degree conclusion work |
1. |
João Silva, Resistência à Malária - prevalência da deficiência em glucose-6-fosfato-desidrogenase (G6PD) na população humana da Ilha de
Santiago, República de Cabo Verde, 2007. Trabalho de Conclusão de Curso (Licenciatura em Licenciatura em Bioquímica) - Universidade de Coimbra (Orientador). |
2. |
Nilza Gonçalves, Prevalência de Drepanocitose na população da Ilha de Santiago, República de Cabo Verde, 2006. Trabalho de Conclusão de Curso (Licenciatura em Licenciatura em Biologia) - Universidade de Coimbra (Orientador). |
3. |
Cristina Mendes, Contribuição para o estudo do efeito da infecção simultânea por P. falciparum e P. vivax no ciclo esporogónico em mosquitos
Anopheles albimanus, 2005. Trabalho de Conclusão de Curso (Licenciatura em BSc in Applied Chemistry) - Universidade Nova de Lisboa (Orientador). |
4. |
Ana Luísa Roque, Transmissão de malária humana: estudo dos estadios transmissíveis (gametócitos) das diferentes espécies de Plasmodium no sangue
periférico, 2004. Trabalho de Conclusão de Curso (Licenciatura em Biochemistry) - Universidade de Lisboa (Orientador). |
5. |
José Luís Vicente, Caracterização genética de populações do parasita da malária Plasmodium falciparum nas ilhas de São Tomé e Príncipe, África
Ocidental, 2004. Trabalho de Conclusão de Curso (Licenciatura em BSc in Technological Engineering) - Universidade Lusófona de Humanidades e Tecnologias (Co-orientador). |
6. |
Sónia Cardoso, Análise de infecções simples e mistas de Plasmodium em mosquitos Anopheles na Ilha Josina (Estação seca) – Distrito da Manhiça,
Moçambique, 2003. Trabalho de Conclusão de Curso (Licenciatura em BSc in Applied Chemistry) - Universidade Nova de Lisboa (Orientador). |
7. |
Dinora Lopes, Avaliação do nível de inibição da “Polymerase Chain Reaction” utilizada na detecção de Plasmodium no mosquito vector Anopheles., 1998. Trabalho de Conclusão de Curso (Licenciatura em BSc in Technological Engineering) - Universidade Lusófona de Humanidades e Tecnologias (Co-orientador). |
8. |
Ana Teresa Correia, Optimização das condições de armazenamento para a detecção de Plasmodium sp. por PCR em amostras sanguíneas. , 1998. Trabalho de Conclusão de Curso (Licenciatura em Applied Plant Biology) - Universidade de Lisboa (Co-orientador). |
Iniciação científica Scientific initiation |
1. |
Cláudia Gomes, Erythrocytic deficiencies and protection against malaria, 2009. Iniciação científica (Biomedical Sciences) - Universidade Nova de Lisboa, Bolseiro(a) de Universidade Nova de Lisboa (Orientador). Grant of Research Initiation. |
2. |
Cristina Mendes, Optimization of Real-Time PCR technique for the semi-quantification of schizogonic stages of the four human Plasmodium species
in peripheral blood when in mixed infection, 2006. Iniciação científica (BSc in Applied Chemistry) - Universidade Nova de Lisboa (Orientador). Training integrated in the Discipline of "Estágio II" of the BSc in Applied Chemistry, Faculty of Sciences and Technology,
Universidade Nova de Lisboa. Classification 18/20. |
Orientação de outra natureza Other orientation |
1. |
Joana Figueiredo, Estágio voluntário no âmbito da linha de investigação "Diversidade populacional e dinâmica de transmissão de Plasmodium sp.
e Plasmodium falciparum na Guiné-Equatorial.", 2010. Universidade de Lisboa (Orientador). |
2. |
Marta Remédios, Estágio voluntário no âmbito do projecto Avaliação da epidemiologia da malária na República de Cabo Verde., 2008. Universidade de Lisboa (Orientador). |
3. |
Patrícia Machado, Evaluation of malaria epidemiology in Republic of Cabo Verde, 2007. Universidade Nova de Lisboa, Bolseiro(a) de Universidade Nova de Lisboa (Orientador). Research Grant. Project "Evaluation of malaria epidemiology in Republic of Cabo Verde” (FCT/MCTES, POCI/SAU-ESP/55110/2004). |
4. |
Valter F. Andrade Neto, Actividade antimalárica experimental de extractos vegetais e moléculas isoladas como esquizonticidas sanguíneos e teciduais, 2004. Universidade Federal de Minas Gerais. |
5. |
Patrícia Matos Marques, Mixed infections in malaria: studies on transmission and interaction, 2004. Universidade Nova de Lisboa, Bolseiro(a) de Universidade Nova de Lisboa (Orientador). Research Grant. Project “Mixed infections in malaria: studies on transmission and interaction” (FCT, POCTI/1999/ESP/35789/99). |
6. |
Vera Valadão Pinto, Mixed infections in malaria: studies on transmission and interaction, 2004. Universidade Nova de Lisboa, Bolseiro(a) de Universidade Nova de Lisboa (Orientador). Research Grant. Project “Mixed infections in malaria: studies on transmission and interaction” (FCT, POCTI/1999/ESP/35789/99). |
7. |
Catarina Casimiro, Estudo da actividade antimalárica de derivados da primaquina no modelo Anopheles stephensi – Plasmodium berghei, 2002. Universidade Nova de Lisboa. |
8. |
João Ferreira, Contributo para o estudo das proteases de Plasmodium chabaudi. Novos alvos para a terapia da malária, 2001. Universidade de Lisboa. |
9. |
Bruno Jesuíno, Efeito de anticorpos anti-Plasmodium na expressão dos genes que codificam a proteína circunsporozoítica (CSP) e proteína do
tipo 1 da superfície do merozoíto (MSP-1) de Plasmodium falciparum, 2001. Universidade Nova de Lisboa. |
10. |
Ana Sofia Franco, Estudos de marcadores genéticos de Plasmodium sp. em duas fases do seu ciclo biológico, 1999. |
11. |
Natércia Fernandes, Contribuição para o estudo da diversidade genética da população parasitária (Plasmodium falciparum), em mulheres grávidas, 1999. |
1. |
Mónica Guerra, Factores que afectam a dinâmica de infecções concomitantes por Plasmodium numa área endémica: análise através de uma nova
abordagem de modelação” (FCT/MCTES, PTDC/SAU-EPI/113326/2009)., 2012. Universidade Nova de Lisboa, Bolseiro(a) de Fundação para a Ciência e a Tecnologia (Orientador). |
2. |
Patrícia Machado, Alterações nas Vias Glicolítica e Pentoses-Fosfato do eritrócito - como afectam a infecção por Plasmódio?, 2011. Universidade Nova de Lisboa, Bolseiro(a) de Universidade Nova de Lisboa (Orientador). Research Grant. Project "Disorders on the Glycolytic and Pentose Phosphate Pathways of the Red Blood Cell - how do they affect
Plasmodium infection?" (FCT/MCTES, PTDC/SAU-MET/110323/2009). |
Outros Trabalhos Other production |
1. |
Arez, Ana P. Molecular epidemiology on malaria. Transmission dynamics of mixed infections., 2000. Dissertation submitted to the Faculty of Sciences, University of Lisbon. Degree of Doctor in Biology (Molecular Biology).
246pp. |
2. |
Arez, Ana P. Aspects of age determination, growth and longevity in three populations of Rana perezi (Seoane, 1885). , 1992. BSc report submitted to the Faculty of Sciences, University of Lisbon. Degree of BSc in Biology. 131pp. |
Participação no júri de Graus Académicos Academic Degrees jury participation |
1. |
Ana Paula Arez; João Rodrigues; Maria do João Prata; Octávio Paulo; Rui Vitorino; Licínio Manco; Miguel de Oliveira Correia. (Patrícia Machado) Pyruvate kinase and glucose-6-phosphate dehydrogenase deficiencies and their association with malaria –
population genetics and proteomic studies, 2013. |
2. |
Ana Paula Arez; António Sarmento; Francisco Antunes; Filomeno Fortes; Helena Agostinho; Virgílio E. do Rosário; Luís Varandas; Jorge Atouguia; Miguel de Oliveira Correia. Participação no júri de Fernanda Dias. Efeito de factores do hospedeiro e parasitários na susceptibilidade à malária e gravidade da doença, 2012. Tese (Biomedical Sciences) - Universidade Nova de Lisboa. Tropcal Medicine. IHMT, University NOVA of Lisbon, May 2012. Co-supervisor. |
3. |
Ana Paula Arez; Paula Faustino; João Pinto; António Amorim; José Rueff; Virgílio E. do Rosário. Participação no júri de Joana Alves. Epidemiologia da malária em Santiago, Cabo Verde – factores genéticos humanos e estrutura populacional do mosquito vector, 2011. Tese (Biomedical Sciences) - Universidade Nova de Lisboa. PhD in Biomedical Sciences. Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa. May 2011. Supervisor. |
1. |
Ana Paula Arez; Jorge Rocha; Licínio Manco; Paulo Almeida. Participação no júri de Mónica Guerra. Pesquisa de assinaturas de selecção de malária na região do gene humano tpi (triose fosfato isomerase, 2012. Dissertação (MSc in Medical Parasitology) - Universidade Nova de Lisboa. Medical Parasitology. IHMT, University NOVA of Lisbon, December 2012. Supervisor. |
2. |
Ana Paula Arez; Fátima Nogueira; Teresa Rebelo; Deodália Dias. Participação no júri de Ana Margarida Rosa. Pyruvate kinase deficiency protection against malaria: in vitro evaluation of 2,3-diphosphoglycerate toxicity and Plasmodium
falciparum susceptibility, 2011. Dissertação (Human Biology and Environment) - Universidade de Lisboa. Human Biology and Environment. Faculty of Sciences, University of Lisbon, February 2010. Supervisor. |
3. |
Ana Paula Arez; Octávio Paulo; Deodália Dias. Participação no júri de Isadora Monteiro. Genetic diversity of Plasmodium falciparum CSA-binding domains involved in placental malaria, 2010. Dissertação (Human Biology and Environment) - Universidade de Lisboa. MSc in Human Biology and Environment. Faculty of Sciences, University of Lisbon. February 2010. Examiner. |
4. |
Ana Paula Arez; João Pinto; Teresa Rebelo; Deodália Dias. Participação no júri de Vânia Teófilo. Estudo da resistência aos antimálaricos sulfadoxina e pirimetamina em Plasmodium falciparum nas ilhas de São Tomé e Príncipe:
origem das mutações pontuais nos genes dhfr e dhps, 2008. Dissertação (Human Biology and Environment) - Universidade de Lisboa. MSc in Human Biology and Environment. Faculty of Sciences, University of isbon, November 2008. Examiner. |
5. |
Ana Paula Arez. Participação no júri de Bianor Valente. Natural frequency of polymorphisms linked to the Chondroitin 4- Sulfotransferase genes and its association with Placental
Malaria, 2008. Dissertação (Human Molecular Biology) - Universidade de Lisboa. MSc in Human Molecular Biology. Faculty of Sciences, University of Lisbon. November 2008. Examiner. |
6. |
Ana Paula Arez; Maria Emília Valadas; Maria Amélia Grácio. Participação no júri de Juliana Miranda. Estudo de determinantes genéticos de susceptibilidade/resistência em crianças com diferentes níveis clínicos de gravidade
de malária (Luanda, Angola), 2007. Dissertação (MSc in Medical Parasitology) - Universidade Nova de Lisboa. MSc in Medical Parasitology. Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa. January 2007. Expert. |
7. |
Ana Paula Arez. Participação no júri de Cristina Mendes. Associação entre as populações parasitárias de Plasmodium sp. e polimorfismos eritrocitários – drepanocitose e deficiência
em glucose-6-fosfato desidrogenase - em Angola, 2007. Dissertação (MSc in Biotechnology) - Universidade Nova de Lisboa. MSc in Biotechnology. Faculty of Sciences and Technology, Universidade Nova de Lisboa, Monte da Caparica, November 2007. Supervisor. |
Bacharelato/Licenciatura Degree of licentiate |
1. |
Ana Paula Arez. Participação no júri de Ana Luísa Roque. Transmissão de malária humana: estudo dos estadios transmissíveis (gametócitos) das diferentes espécies de Plasmodium no sangue
periférico, 2004. Trabalho de Conclusão de Curso (Bacharelato/Licenciatura em Biochemistry) - Universidade de Lisboa. BSc in Biochemistry, Faculty of Sciences, University of Lisbon. January 2005. Supervisor. |
Participação em Comissões de Avaliação Evaluation comissions participation |
1. |
Research technician grant, 2012, Universidade Nova de Lisboa. Project “Functional characterization of receptors from the 2 GPCR family in the malaria model", FCT/MCTES, PTDC/SAU-MET/110323/2009,
Member. (january 2012). |
2. |
Research technician grant, 2012. Project: Factors affecting dynamics of Plasmodium concomitant infections in a malaria endemic area: analysis through a new
modelling approach. FCT/MCTES, PTDC/SAU-EPI/113326/2009, President ( july 2012). |
3. |
Member of a PhD Tutorial Committee, 2011, Universidade Nova de Lisboa. PhD project - The effect of host and parasite factors in susceptibility to malaria severity. MD Fernanda Dias, 2005 - 2011. |
4. |
Member of PhD Tutorial Committee, 2011, Universidade de Lisboa. PhD project - Malaria and Tuberculosis: interactions in the host and the role of the malaria pigment. Rosângela Frita. 2005
- 2011. |
5. |
Research grant, 2011, Universidade Nova de Lisboa. Project “Disorders on the Glycolytic and Pentose Phosphate Pathways of the Red Blood Cell - how do they affect Plasmodium
infection?”, FCT/MCTES, PTDC/SAU-MET/110323/2009, President of juri. (october 2011). |
6. |
Post-doc grant, 2010, Universidade Nova de Lisboa. Project “Immune stimulation of the mosquito Anopheles gambiae response against the malaria parasite Plasmodium berghei”, PTDC/SAU-MII/102596/2008.
Member (July 2010). |
7. |
Research grant, 2010, Universidade Nova de Lisboa. Project “Immune stimulation of the mosquito Anopheles gambiae response against the malaria parasite Plasmodium berghei”, PTDC/SAU-MII/102596/2008.
Member (July 2010). |
8. |
Research technician, 2010, Universidade Nova de Lisboa. Project “Immune stimulation of the mosquito Anopheles gambiae response against the malaria parasite Plasmodium berghei”, PTDC/SAU-MII/102596/2008.
Member (July 2010). |
9. |
Member of a PhD Tutorial Committee, 2009, Universidade Nova de Lisboa. Member of Tutorial Committee of a PhD project - Epidemiological studies and resistance to antimalarials in East Timor. Afonso
de Almeida. 2004 - 2009. |
10. |
Research grant, 2008, Universidade Nova de Lisboa. Project "Genetic variability of recognition protein expressing genes (PRM) involved in the mosquito immune response against
Plasmodium infection”, POCTI/SAU-IMI/59489/2004 Member (April 2008). |
11. |
Research grant, 2006, Universidade Nova de Lisboa. Project "Genetic variability of recognition protein expressing genes (PRM) involved in the mosquito immune response against
Plasmodium infection”, POCTI/SAU-IMI/59489/2004 Member (November 2006). |
12. |
Research grant, 2005, Universidade Nova de Lisboa. Project “Population structure and genetic efflux of malaria parasites in islands”, POCTI/SAU-ESP/56903/2004, Member (November
2005). . |
13. |
Research grant, 2005, Universidade Nova de Lisboa. Project "Genetic variability of recognition protein expressing genes (PRM) involved in the mosquito immune response against
Plasmodium infection”, POCTI/SAU-IMI/59489/2004 Member (November 2005). . |
14. |
Research grant, 2005, Universidade Nova de Lisboa. Project "Evaluation of malaria epidemiology in the Republic of Cabo Verde”, POCI/SAU-ESP/55110/2004. President (September
2005). |
15. |
Research grant, 2003, Universidade Nova de Lisboa. Project "Study of gene expression during sporogonic cycle of Plasmodium: effect of antibodies and antimalarials", POCTI/MGI/35815/00,
Member (May 2003). |
16. |
Research grant, 2002, Universidade Nova de Lisboa. Project "Study of genetic resistance to antimalarials", POCTI/ESP/42666/2001, Member (August 2002). |
17. |
Research grant, 2002, Universidade Nova de Lisboa. Project “Mixed infections in malaria: studies on transmission and interaction”, POCTI/1999/ESP/35789/99, President (August
2002). |
18. |
Research grant, 2002, Universidade Nova de Lisboa. Project "Study of the effect of antibodies anti-plasmodia in the development and gene expression of the parasite in the mosquito
vector", POCTI/ESP/43636/99, Member (January 2002). |
19. |
Research grant, 2001, Universidade Nova de Lisboa. Project "Study of gene expression during sporogonic cycle of Plasmodium: effect of antibodies and antimalarials", POCTI/MGI/35815/00,
Member (May 2001). |
20. |
Research grant, 2001, Universidade Nova de Lisboa. Project "Study of gene expression during sporogonic cycle of Plasmodium: effect of antibodies and antimalarials", POCTI/MGI/35815/00,
Member (November 2001). |
21. |
Research technician, 2001, Universidade Nova de Lisboa. Project "Study of gene expression during sporogonic cycle of Plasmodium: effect of antibodies and antimalarials", POCTI/MGI/35815/00,
Member (April 2001). |
22. |
Research grant, 2001, Universidade Nova de Lisboa. Project “Mixed infections in malaria: studies on transmission and interaction”, POCTI/1999/ESP/35789/99, President (March
2001). |
Participação em eventos Event participation |
Participação como Keynote Speaker Participation as Keynote Speaker |
1 |
Qual o possível impacto das doenças tropicais na Europa?, 2008 (Mesa-Redonda). Nome do evento: Tertúlias Científicas Viana do Castelo; Nome da Instituição: Escola Superior de Enfermagem; Cidade do evento: Viana do Castelo / Escola Superior de Enfermagem. |
2 |
Malária - diversidade parasitária/infecções mistas de Plasmodium sp. - implicações na transmissão e patologia, 2003 (Mesa-Redonda). Nome do evento: VII Congresso Português de Parasitologia; Nome da Instituição: Sociedade Portuguesa de Parasitologia; Cidade do evento: Lisboa / Instituto de Higiene e Medicina Tropical. |
3 |
Chronic infections: malaria, 2003 (Mesa-Redonda). Nome do evento: XXIX Reunião Anual da Sociedade Portuguesa de Imunologia; Nome da Instituição: Sociedade Portuguesa de Imunologia; Cidade do evento: Porto. |
Participação como Moderador Participation as Chairman |
1. |
Malaria, 2013 (Congresso). Nome do evento: 2º Congresso Nacional de Medicina Tropical; Nome da Instituição: IHMT; Cidade do evento: Lisboa / Portugal. |
2. |
Host Genetics Session, 2012 (Congresso). Nome do evento: 11th International Conference on Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases (MEEGID); Nome da Instituição: Elsevier; Cidade do evento: New Orleans / Loyola University. |
Participação como Relator Participation as Commentator |
1 |
Investigação sustentada. Criação de bancos biológicos e sistemas de análise e informação, 2006 (Mesa-Redonda). Nome do evento: I Seminário de Terapêutica da Malária da CPLP; Nome da Instituição: RIDES-Malária, Centro de Malária e outras Doenças Tropicais; Cidade do evento: Lisboa / Instituto de Higiene e Medicina Tropical. |
Participação editorial em revistas Magazine editorial participation |
1. |
Ana Paula Arez. PLoS ONE, Função ou tipo de participação: Reviewer, Editora: PLoS ONE.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
2. |
Ana Paula Arez. PLoS Neglected Tropical Diseases, Função ou tipo de participação: Reviewer, Editora: PLoS.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
3. |
Ana Paula Arez. Journal of Clinical Microbiology, Função ou tipo de participação: Reviewer, Editora: American Society for Microbiology.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
4. |
Ana Paula Arez. Infection and Immunity, Função ou tipo de participação: Reviewer, Editora: American Society for Microbiology.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
5. |
Ana Paula Arez. BMC Infectious Diseases, Função ou tipo de participação: Reviewer, Editora: BioMed Central.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
6. |
Ana Paula Arez. Transactions of The Royal Society of Tropical Medicine and Hygiene, Função ou tipo de participação: Reviewer, Editora: Elsevier Editorial System.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
7. |
Ana Paula Arez. Acta Tropica, Função ou tipo de participação: Reviewer, Editora: Elsevier Editorial System.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
8. |
Ana Paula Arez. Tropical Medicine and International Health, Função ou tipo de participação: Reviewer, Editora: Wiley-Blackwell.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
9. |
Ana Paula Arez. Annals of Tropical Medicine and Parasitology, Função ou tipo de participação: Reviewer, Editora: Maney Publishing.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
10. |
Ana Paula Arez. PLoS ONE, de 2011/09/28 até 2011/12/28, Função ou tipo de participação: Corpo editorial, Editora: PLoS.
![Homepage da produção](/wayback/20171213082241im_/http://www.degois.pt/imagens/degois/home16.png) |
Indicadores de produção
(Production indicators)
Produção científica
Scientific production |
Produção técnica
Technical production |
Dados complementares
data |
Produção científica Scientific production |
50 |
Livros e capítulos Books and book chapters |
1 |
Capítulos de livros publicados Published book chapters |
1 |
Artigos científicos em revistas Papers in periodics |
39 |
Com arbitragem científica With scientific refereeing |
39 |
Trabalhos em eventos Papers in conference proceedings |
9 |
Com arbitragem científica With scientific refereeing |
9 |
Textos em jornais ou revistas Texts in journals or magazines |
1 |
Produção técnica Technical production |
60 |
Outros tipos de produção técnica Other technical production |
60 |
Dados complementares (Additional data) |
86 |
Orientações Orientations |
35 |
Outros Trabalhos Other production |
2 |
Participação no Júri de Graus Académicos Academic Degrees jury participation |
11 |
Participação em Comissões de Avaliação Evaluation comissions participation |
22 |
Participação em Eventos Event participation |
6 |
Participação editorial em revistas Magazine editorial participation |
10 |
Outras informações relevantes
Due to a strong background on Ecology acquired during my BSc degree, my MAIN AREA OF INTEREST has always been parasite epidemiology,
especially malaria, transmission dynamics and interactions and ecological relationships between different parasites co-infecting
the same host. Within this scope, I have conducted several research projects in malaria endemic areas, namely Guinea-Bissau,
Cape Verde, Mozambique and Equatorial Guinea (on-going project). The unexpected observations in Cabo Verde led to a new
line of research - human host factors of susceptibility/ resistance to malaria. So far, encouraging results have been
obtained and new plans and projects for future work are being implemented. I have acquired EXPERIENCE in field work
in endemic areas, both regarding blood and mosquito collections and at the laboratory, I mainly work with in vitro cultures,
manipulation of experimental murine models, molecular techniques such as PCR-based techniques, cloning, sequencing and immuno-enzymatic
techniques. Also experienced in post-graduation training activities both locally and in endemic areas. I am a full
MEMBER of the CMDT-LA (Centre of Malaria and other Tropical Diseases, Institute of Hygiene and Tropical Diseases, University
Nova of Lisbon) and Collaborator of CIAS (Centre of Research for Anthropology and Health, Faculty of Sciences and Technology,
University of Coimbra) Also, several COOPERATION activities are carried out, which mainly fall on: 1) Advanced Training of
Human Resources (eg PhD projects - Joana Alves, Ministry of Health of Cabo Verde, Fernanda Dias, Faculty of Medicine, University
Agostinho Neto, Angola, Afonso de Almeida, Timor Lorosae; MSc projects - Juliana Miranda, Paediatric Hospital David Bernardino,
Angola, Joana Alves, Ministry of Health of Cabo Verde); 2) activity integrated on projects (eg training of technicians in
Chokwe, Mozambique, under the "Project to Integrated Malaria Control Program in Region of (Gaza)"; also in Mozambique, Faculty
of Medicine, University Eduardo Mondlane (Maputo) with a) technology transfer for erythrocyte enzyme activity determinations,
b) detection of the sickle cell trait by genotyping human HBB, c) detection of Plasmodium in peripheral blood from blood bank
donors; in Cabo Verde, General Directorate of Health, when a mission was carried out (November/December 2007), involving two
projects (POCI/SAU-ESP/55110/2004 and POCI/BIA-BDE/57650/2004) – an entomological survey was made and results reported to
the General Directorate of Health – among others, these included a detailed description of the distribution of the vector
Aedes aegypti, very relevant for the later dengue outbreak management in 2009.
|
Visualizações do curriculum [
2672
]
|
Página gerada pela Plataforma de Curricula DeGóis promovida pela FCT e pelo Gávea/DSI/UM
em
13-12-2017
às
08:22:43
|
Plataforma de Curricula DeGóis: http://www.degois.pt | Icons by Axialis Team |
|