Luís Pereira de Almeida (luispa@ci.uc.pt) graduated in Pharmaceutical Sciences at University of Coimbra in 1991. After 2 years as chief of production at Laboratorios Delta, Rotta Research Group in Queluz, later integrated the Faculty of Pharmacy as teaching assistant and developed a master thesis on modeling dissolution of drugs. Later, with a fellowship from the Portuguese Foundation for Science and technology developed his PhD project at the Gene Therapy Center, Centre Hôpitalier Universitaire Vaudois and Faculty of Medicine in Lausanne, Switzerland from 1998 to 2001. Assistant professor at the Faculty of Pharmacy, University of Coimbra since 2003, teaches at graduate and undergraduate levels and has coordinated the Masters in Pharmaceutical Biotechnology. His research activity is developed at the Center for Neuroscience and Cell Biology of the University of Coimbra (CNC), Portugal where he is principal investigator and member of the board. He spent short sabbatical leaves at CEA, Saclay in France (2005) and at the Massachussetts Institute of Technology (2010). The research of his group has been focused on gene therapy approaches for neurodegenerative disorders with a focus on Machado-Joseph disease/spinocerebellar ataxia type 3, including disease modifying and gene silencing approaches, autophagy activation and proteolysis inhibition, works published in over thirty papers cited over 700 times, in journals such as Brain, Annals of Neurology, Human Molecular Genetics, PLoS One, Human Gene Therapy and The Journal of Neuroscience, awarded with prizes by the Portuguese Society for Neurosciences (2009, 2011, 2012), the Portuguese Society of Human Genetics (2009), and Fundação Pulido Valente. For some years coordinated the Doctoral Program in Experimental Biology and Biomedicine of CNC and has been organizing advanced courses on Neurodegenerative diseases, Principles and Practice in Drug Development (MIT Portugal -PhD Program), Nuclear biology and gene therapy and Gene and cell therapy for the CNS. Luis Pereira de Almeida is or has been responsible for 15 research projects funded by the Portuguese Foundation for Science and Technology, the Association Française de Myopathies, private funds, the National Ataxia Foundation (USA) and leads one of the twelve European research groups that constitute the Initial Training Network TreatPolyQ within the 7th Framework Program of the European Union.
Endereço de acesso a este CV: http://www.degois.pt/visualizador/curriculum.jsp?key=0735387574214965

Nome completo Full name	Luis Fernando Morgado Pereira de Almeida
Nome em citações bibliográficas Quoting name	Pereira de Almeida, Luis Fernando Morgado
Categoria profissional Position	Professor Auxiliar
Domínio científico de atuação Scientific domain	Ciências Médicas-Biotecnologia Médica. Engenharia e Tecnologia-Nanotecnologia. Ciências Médicas-Outras Ciências Médicas. Ciências Naturais-Ciências Biológicas. Ciências Médicas-Ciências da Saúde.

Endereço profissional Professional address

Universidade de Coimbra Faculdade de Farmácia Pólo das Ciências da Saúde Azinhaga de Santa Comba Coimbra 3000-548 Coimbra Portugal Telefone: (+351)239488400 Correio electrónico: luispa@ci.uc.pt

Sexo Gender

Masculino»Male

1997-2003	Doutoramento Phd
Doutoramento em Tecnologia Farmacêutica. Universidade de Coimbra, Portugal.

1993-1996	Mestrado Master degree
Mestrado em Tecnologias do Medicamento (2 anos » years) . Universidade de Coimbra, Portugal.

1985-1991	Licenciatura Licentiate degree
Licenciatura em Ciências Farmacêuticas (6 anos » years) . Universidade de Coimbra, Portugal.

Centro de Neurociências e Biologia Celular da Universidade de Coimbra

Fev/2008-Actual

Professor Auxiliar

Universidade de Coimbra

Fev/2008-Actual

Professor Auxiliar

Fev/2003-Fev/2008

Professor Auxiliar

Jan/1996-Fev/2003

Assistente

Mar/1993-Jan/1996

Assistente Estagiário

Universite de Lausanne

Fev/1998-Abr/2001

Assistente

Compreende Understandig	Português (Bem), Francês (Bem), Inglês (Bem), Espanhol (Razoavelmente).
Fala Speaking	Português (Bem), Francês (Bem), Inglês (Bem), Espanhol (Pouco).
Lê Reading	Português (Bem), Francês (Bem), Inglês (Bem), Espanhol (Razoavelmente).
Escreve Writing	Português (Bem), Francês (Bem), Inglês (Bem), Espanhol (Pouco).

2009	Research Award -Project:"Modulation of autophagy as a novel therapeutic strategy for Machado-Joseph disease"., National Ataxia Foundation, USA/ Duration: 2010 .
2004	Research award: -"Lentiviral vector-based silencing of endogenous rat ataxin-3", Reference #8 04/05, National Ataxia Foundation, USA.
2010	Melhor trabalho português publicado em 2008 com impacto na área da genética humana e/ou médica atribuído ao orientando Sandro Alves, pelo artigo “Allele-specific RNA silencing of mutant ataxin-3..." Sociedade Portug, Sociedade Portuguesa de Genética Humana.
2009	Artigo Destaque_SPN2009 - atribuído ao primeiro autor e orientando Sandro Alves pelo artigo Striatal and nigral pathology in a lentiviral rat model of Machado-Joseph disease. Hum Mol Genet. 2008; 17: 2071-2083 , Sociedade Portuguesa de Neurociencias.
2011	Artigo Destaque_SPN2011 - atribuído ao primeiro autor e orientando Sandro Alves pelo artigo Silencing ataxin-3 mitigates degeneration in a rat model of Machado-Joseph disease: no role for wild-type ataxin-3? Human Molecular Genetics 2010 Apr 9, Sociedade Portuguesa de Neurociências.

Actual	Sociedade Portuguesa de Bioquímica, Membro.
Actual	Sociedade Portuguesa de Neurociências, Membro.
Actual	Sociedade Portuguesa de Células Estaminais e Terapia Celular (SPCE-TC), Membro fundador.
Actual	Society of Controlled Release , Membro.
Actual	Society for Neurosciences (SFN) - Sociedade Americana de Neurociências, Membro.
Actual	European Society for Gene and Cell Therapy, Membro.
Jun/1991 - Actual	Ordem dos Farmacêuticos, Membro. Membro da Ordem dos Farmacêuticos nº C-1522 (Carteira Profissional nº 08998)..

Capítulos de livros publicados Published book chapters

1.	C. Cardoso, A. L; Trabulo, S; Pereira de A. L. F. M; Pedroso de L. M. C. 2012. Interaction of nucleic acids with biological membranes: activation of inflammatory mechanisms.  In A Toxicological / Pharmacological Approach to the Chemico-Biological Interactions at the Membrane Level, 0 - 0. . 0: Translational Research Network.

2.	Pereira de A. L. F. M; Nobrega, C.. 2012. Spinocerebellar Ataxia, Jose Gazulla (Ed.), ISBN: 978-953-51-0542-8, InTech, .  In Spinocerebellar Ataxia, ed. Jose Gazulla (Ed.), 0 - 0. ISBN: 978-953-51-0542-8. 0: In Tech.

3.	C. Cardoso, A. L; Trabulo, S; Duarte, Sara; Costa, P.; Pereira de A. L. F. M; Pedroso de L. M. C. 2010. Gene silencing as a promising strategy to target CNS and neurological disorders.  In Gene Silencing: Theory, Techniques and Applications, 0 - 0. . 0: 0.

4.	Ribeiro, Sandra M; Almeida, Luís P.; Carvalho, Ana L; Rego, Ana C. 2007. Polyglutamine Expansion Diseases – the Case of Machado-Joseph Disease.  In Interaction Between Neurons and Glia in Aging and Disease, ed. João O. Malva, Ana Cristina Rego, Rodrigo A. Cunha, Catarina R. Oliveira, 391 - 426. ISBN: 978-0-387-70829-4. Boston, MA: Springer US.

Artigos em revistas com arbitragem científica Papers in periodics with scientific refereeing

1.	Nóbrega, Clévio; Nascimento-Ferreira, Isabel; Onofre, Isabel; Albuquerque, David; Hirai, Hirokazu; Déglon, Nicole; de Almeida, L. P; Chiesa, Roberto. 2013. "Silencing Mutant Ataxin-3 Rescues Motor Deficits and Neuropathology in Machado-Joseph Disease Transgenic Mice", PLoS ONE 8, 1: e52396 - e52396.

2.	Gonçalves, Nélio; Simões, Ana T; Cunha, Rodrigo A; de Almeida, L. P. 2013. "Caffeine and adenosine A2A receptor inactivation decrease striatal neuropathology in a lentiviral-based model of Machado-Joseph disease ", Annals of Neurology 73, 5: 655 - 666.

3.	Nascimento-Ferreira, I.; Nobrega, C.; Vasconcelos-Ferreira, A.; Onofre, I.; Albuquerque, D.; Aveleira, C.; Hirai, H.; Deglon, N.; Pereira de A. L. 2013. "Beclin 1 mitigates motor and neuropathological deficits in genetic mouse models of Machado-Joseph disease", Brain 136, 7: 2173 - 2188.

4.	Costa, Pedro M; Cardoso, Ana L; Mendonça, Liliana S; Serani, Angelo; Custódia, Carlos; Conceição, Mariana; Simões, Sérgio; Moreira, João N; Pereira de A. L; Pedroso de L. M. C. 2013. "Tumor-targeted Chlorotoxin-coupled Nanoparticles for Nucleic Acid Delivery to Glioblastoma Cells: A Promising System for Glioblastoma Treatment", Molecular Therapy—Nucleic Acids 2, 6: e100 - e100.

5.	Sousa-Ferreira, Lígia; Almeida, Luís P.; Cavadas, Cláudia. 2013. "Role of hypothalamic neurogenesis in feeding regulation", Trends in Endocrinology & Metabolism, .: 1 - 9.

6.	Lopes, Carla; Ribeiro, Márcio; Duarte, Ana I; Humbert, Sandrine; Saudou, Frederic; Pereira de A. L; Hayden, Michael; Rego, A. C. 2013. "IGF-1 Intranasal Administration Rescues Huntington's Disease Phenotypes in YAC128 Mice", Molecular Neurobiology, 0: 0 - 0.

7.	Cardoso, Ana L; Guedes, Joana R; Pereira de A. L; Pedroso de L. M. C. 2012. "miR-155 modulates microglia-mediated immune response by down-regulating SOCS-1 and promoting cytokine and nitric oxide production", Immunology 135, 1: 73 - 88.

8.	Simoes, A. T; Goncalves, N.; Koeppen, A.; Deglon, N.; Kugler, S.; Duarte, C. B; Pereira de A. L. 2012. "Calpastatin-mediated inhibition of calpains in the mouse brain prevents mutant ataxin 3 proteolysis, nuclear localization and aggregation, relieving Machado-Joseph disease", Brain 135, 8: 2428 - 2439.

9.	Costa, P. M; Cardoso, A. L; Nobrega, C.; Pereira de A. L. F; Bruce, J. N; Canoll, P.; Pedroso de L. M. C. 2012. "MicroRNA-21 silencing enhances the cytotoxic effect of the antiangiogenic drug sunitinib in glioblastoma", Human Molecular Genetics, 0: 0 - 0.

10.	Costa, P. M; Cardoso, A. L; Pereira de A. L. F; Bruce, J. N; Canoll, P.; Pedroso de L. M. C. 2012. "PDGF-B-mediated downregulation of miR-21: new insights into PDGF signaling in glioblastoma", Human Molecular Genetics 0, 0: 0 - 0.

11.	Nóbrega, Clévio; Nascimento-Ferreira, Isabel; Onofre, Isabel; Albuquerque, David; Conceição, Mariana; Déglon, Nicole; Almeida, Luís P. 2012. "Overexpression of Mutant Ataxin-3 in Mouse Cerebellum Induces Ataxia and Cerebellar Neuropathology", The Cerebellum, 0: 0 - 0.

12.	Nascimento-Ferreira, I.; Santos-Ferreira, T.; Sousa-Ferreira, L.; Auregan, G.; Onofre, I.; Alves, S.; Dufour, N.; Colomer Gould, V. F; Koeppen, A.; Deglon, N.; Pereira de A. L. 2011. "Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease", Brain 134, 5: 1400 - 1415.

13.	Gomes, J. R; Lobo, A. C; Melo, C. V; Inacio, A. R; Takano, J.; Iwata, N.; Saido, T. C; de Almeida, L. P; Wieloch, T.; Duarte, C. B. 2011. "Cleavage of the Vesicular GABA Transporter under Excitotoxic Conditions Is Followed by Accumulation of the Truncated Transporter in Nonsynaptic Sites", Journal of Neuroscience 31, 12: 4622 - 4635.

14.	Sousa-Ferreira, Lígia; Garrido, Manuel; Nascimento-Ferreira, Isabel; Nobrega, Clévio; Santos-Carvalho, Ana; ¿?lvaro, Ana R; Rosmaninho-Salgado, Joana; Kaster, Manuella; Kügler, Sebastian; Pereira de A. L; Cavadas, Claudia; Tomé, Daniel. 2011. "Moderate Long-Term Modulation of Neuropeptide Y in Hypothalamic Arcuate Nucleus Induces Energy Balance Alterations in Adult Rats", PLoS ONE 6, 7: 22333 - 22333.

15.	Sousa-Ferreira, Lígia; ¿?lvaro, Ana R; Aveleira, Célia; Santana, Magda; Brandão, Inês; Kügler, Sebastian; Pereira de A. L; Cavadas, Cláudia; Linden, Rafael. 2011. "Proliferative Hypothalamic Neurospheres Express NPY, AGRP, POMC, CART and Orexin-A and Differentiate to Functional Neurons", PLoS ONE 6, 5: 19745 - 19745.

16.	Jorge Nobre, R; Pereira de A. L. 2011. "Gene Therapy for Parkinson's and Alzheimer's Diseases: from the Bench to Clinical Trials", Current Pharmaceutical Design 17, 31: 3434 - 3445.

17.	Alves, S.; Nascimento-Ferreira, I.; Dufour, N.; Hassig, R.; Auregan, G.; Nobrega, C.; Brouillet, E.; Hantraye, P.; Pedroso de L. M. C; Deglon, N.; de Almeida, L. P. 2010. "Silencing ataxin-3 mitigates degeneration in a rat model of Machado-Joseph disease: no role for wild-type ataxin-3?", Human Molecular Genetics 19, 12: 2380 - 2394.

18.	Cardoso, A.L.C.; Costa, P.; de Almeida, L; Simões, S.; Plesnila, N.; Culmsee, C.; Wagner, E.; Pedroso de L. M. 2010. "Tf-lipoplex-mediated c-Jun silencing improves neuronal survival following excitotoxic damage in vivo", Journal of Controlled Release 142, 3: 392 - 403.

19.	Nobre, Rui J; Cruz, Eugénia; Real, Odete; de Almeida, L. P; Martins, Teresa C. 2010. "Characterization of common and rare human papillomaviruses in Portuguese women by the polymerase chain reaction, restriction fragment length polymorphism and sequencing", Journal of Medical Virology 82, 6: 1024 - 1032.

20.	Alves, Sandro; Nascimento-Ferreira, Isabel; Auregan, Gwennaëlle; Hassig, Raymonde; Dufour, Noëlle; Brouillet, Emmanuel; Pedroso de L. M. C; Hantraye, Philippe; Pereira de A. L; Déglon, Nicole; Cookson, Mark R. 2008. "Allele-Specific RNA Silencing of Mutant Ataxin-3 Mediates Neuroprotection in a Rat Model of Machado-Joseph Disease", PLoS ONE 3, 10: 3341 - 3341.

21.	Cardoso, A.L.C.; Simões, S.; de Almeida, L; Plesnila, N.; Pedroso de L. M; Wagner, E.; Culmsee, C.. 2008. "Tf-lipoplexes for neuronal siRNA delivery: A promising system to mediate gene silencing in the CNS", Journal of Controlled Release 132, 2: 113 - 123.

22.	Alves, S.; Regulier, E.; Nascimento-Ferreira, I.; Hassig, R.; Dufour, N.; Koeppen, A.; Carvalho, A. L; Simoes, S.; de Lima, M. C. P; Brouillet, E.; Gould, V. C; Deglon, N.; de Almeida, L. P. 2008. "Striatal and nigral pathology in a lentiviral rat model of Machado-Joseph disease", Human Molecular Genetics 17, 14: 2071 - 2083.

23.	Nobre, Rui J; de Almeida, L. P; Martins, Teresa C. 2008. "Complete genotyping of mucosal human papillomavirus using a restriction fragment length polymorphism analysis and an original typing algorithm", Journal of Clinical Virology 42, 1: 13 - 21.

24.	Vieira, Otilia V; Hartmann, Diego O; Cardoso, Carla M. P; Oberdoerfer, Daniel; Baptista, Marta; Santos, Manuel A. S; Almeida, Luis; Ramalho-Santos, João; Vaz, Winchil L. C; Bahn, Yong-Sun. 2008. "Surfactants as Microbicides and Contraceptive Agents: A Systematic In Vitro Study", PLoS ONE 3, 8: 2913 - 2913.

25.	Cardoso, A. L. C; Simões, S.; de Almeida, L. P; Pelisek, J.; Culmsee, C.; Wagner, E.; Pedroso de L. M. C. 2007. "siRNA delivery by a transferrin-associated lipid-based vector: a non-viral strategy to mediate gene silencing", The Journal of Gene Medicine 9, 3: 170 - 183.

26.	Teresa Girão C. M; Cardoso, A L. C; de Almeida, L. P; Simões, S; Pedroso de L. M. C. 2005. "Tf-lipoplex-mediated NGF gene transfer to the CNS: neuronal protection and recovery in an excitotoxic model of brain injury", Gene Therapy 12, 16: 1242 - 1252.

27.	Rego, A. C; de Almeida, L. P. 2005. "Molecular Targets and Therapeutic Strategies in Huntington's Disease", Current Drug Targets - CNS & Neurological Disorders 4, 4: 361 - 381.

28.	P. de L. M. C; Girao da C. M. T; C. Cardoso, A. L; Simoes, S.; de Almeida, L. P. 2005. "Liposomal and Viral Vectors for Gene Therapy of the Central Nervous System", Current Drug Targets - CNS & Neurological Disorders 4, 4: 453 - 465.

29.	Zala, Diana; Bensadoun, Jean-Charles; Pereira de A. L; Leavitt, Blair R; Gutekunst, Claire-Anne; Aebischer, Patrick; Hayden, Michael R; Déglon, Nicole. 2004. "Long-term lentiviral-mediated expression of ciliary neurotrophic factor in the striatum of Huntington's disease transgenic mice", Experimental Neurology 185, 1: 26 - 35.

30.	Bensadoun, Jean-Charles; Pereira de A. L; Fine, Eric G; Tseng, Jack L; Déglon, Nicole; Aebischer, Patrick. 2003. "Comparative study of GDNF delivery systems for the CNS: polymer rods, encapsulated cells, and lentiviral vectors", Journal of Controlled Release 87, 1-3: 107 - 115.

31.	Régulier, E.; Pereira de A. L; Sommer, B.; Aebischer, P.; Déglon, N.. 2002. "Dose-Dependent Neuroprotective Effect of Ciliary Neurotrophic Factor Delivered via Tetracycline-Regulated Lentiviral Vectors in the Quinolinic Acid Rat Model of Huntington's Disease", Human Gene Therapy 13, 16: 1981 - 1990.

32.	Pereira de A. L; Ross, C; Zala, D.; Aebischer, P.; Déglon, Nicole. 2002. "Lentiviral-mediated delivery of mutant huntingtin in the striatum of rats induces a selective neuropathology modulated by polyglutamine repeat size, huntingtin expression levels, and protein length", The Journal of Neuroscience 22, 9: 3473 - 3483.

33.	de Almeida, L; Zala, D.; Aebischer, P.; Déglon, N.. 2001. "Neuroprotective Effect of a CNTF-Expressing Lentiviral Vector in the Quinolinic Acid Rat Model of Huntington's Disease", Neurobiology of Disease 8, 3: 433 - 446.

34.	Bensadoun, Jean-Charles; de Almeida, L. P; Dreano, Michel; Aebischer, Patrick; Deglon, Nicole. 2001. "Neuroprotective effect of interleukin-6 and IL6/IL6R chimera in the quinolinic acid rat model of Huntington's syndrome", European Journal of Neuroscience 14, 11: 1753 - 1761.

35.	Deglon, Nicole; Tseng, Jack L; Bensadoun, Jean-Charles; Zurn, Anne D; Arsenijevic, Yvan; Almeida, Luis P. D; Zufferey, Romain; Trono, Didier; Aebischer, Patrick. 2000. "Self-Inactivating Lentiviral Vectors with Enhanced Transgene Expression as Potential Gene Transfer System in Parkinson's Disease", Human Gene Therapy 11, 1: 179 - 190.

36.	De Almeida, L. P; Sim¿es, S¿rgio; Brito, Paulo; Portugal, Ant¿nio; Figueiredo, Margarida. 1997. "Modeling dissolution of sparingly soluble multisized powders", Journal of Pharmaceutical Sciences 86, 6: 726 - 732.

37.	Simöes, Sérgio; Pereira de A. L; Figueiredo, Margarida. 1996. "Testing the applicability of classical diffusional models to polydisperse systems", International Journal of Pharmaceutics 139, 1-2: 169 - 176.

Artigos em revistas sem arbitragem científica Papers in periodics without scientific refereeing

1.	Pereira de A. L. F. M; Nascimento-Ferreira, I.. 2011. "Beclin-1 and autophagy: potential for new therapeutics in neurodegeneration", Neurodegenerative Disease Management 1, 5: 349 - 351.

2.	Pereira de A. L. F. M. 2011. "Células Estaminais Pluripotentes Induzidas (IPSCS) – A mais recente revolução das ciências da vida e da saúde", BOLETIM DO CIM - ROF 97 Mar/Abr 2011, 97: 5 - 6.

Produção científica Scientific production

Total

Produção científica Scientific production

43

Livros e capítulos Books and book chapters

4

Capítulos de livros publicados Published book chapters

4

Artigos científicos em revistas Papers in periodics

39

Com arbitragem científica With scientific refereeing

37

Sem arbitragem científica Without scientific refereeing

2

	Ana dos Santos Carvalho
	Ana Luísa Colaço Cardoso
	Ana Luisa Monteiro de Carvalho
	Carla Maria Nunes Lopes
	Carlos Manuel Tavares da Custódia
	Clévio David Rodrigues Nóbrega
	David dos Santos Albuquerque
	Isabel Maria dos Santos Onofre
	Joana Ribeiro Guedes
	Lígia Maria de Sousa Ferreira
	Márcio José do Coito Ribeiro
	Mariana Oliveira Conceição
	Nélio da Mota Gonçalves
	Pedro Miguel Coutinho da Costa
	Sónia Patrícia Dias Duarte
	Teresa Maria Caldeira Martins