Teodor Adrian Enache. Concluiu Toxicologia Bioquímica pela Universidade de Coimbra em 2011. É do Instituto Pedro Nunes. Publicou 15 artigos em revistas especializadas. Participou em 11 eventos no estrangeiro. Actua nas áreas de Ciências Exactas com ênfase em Química, Ciências Exactas com ênfase em Física e Engenharia e Tecnologia com ênfase em Nanotecnologia. Nas suas actividades profissionais interagiu com 27 colaboradores em co-autorias de trabalhos científicos. No seu curriculum DeGóis os termos mais frequentes na contextualização da produção científica, tecnológica e artístico-cultural são: oxidation, voltammetry, Glassy carbon, Adsorption, Alkaloid, Boron-doped diamond, anti-arrhythmic drug, Aspirin, Berberine e Cysteine.
Endereço de acesso a este CV: http://www.degois.pt/visualizador/curriculum.jsp?key=2125381797470927

Nome completo Full name	Teodor Adrian Enache
Nome em citações bibliográficas Quoting name	Enache, Teodor Adrian
Domínio científico de atuação Scientific domain	Ciências Exactas-Química. Ciências Exactas-Física. Engenharia e Tecnologia-Nanotecnologia.

Endereço profissional Professional address

Instituto Pedro Nunes Laboratório de electroanálise e corrosão Rua pedro Nunes Coimbra 3030-199 Coimbra Portugal Telefone: (+351)239700943 Correio electrónico: teodor@ipn.pt

Sexo Gender

Masculino»Male

2008-2011	Doutoramento Phd
Toxicologia Bioquímica (4 anos » years) . Universidade de Coimbra, Portugal.

2004-2006	Mestrado Master degree
Bíofisica e Física Medica (1,5 anos » years) . Universidade do Bucareste, Faculdade de Fisica, Roménia.

2000-2004	Licenciatura Licentiate degree
Biofísica (4 anos » years) . Universidade do Bucareste, Faculdade de Fisica, Roménia.

Instituto Pedro Nunes

Jan/2012-Actual

Outra Situação

Instituto Pedro Nunes

Jan/2007-Set/2007

Estagiário Investigador

Compreende Understandig	Português (Bem), Inglês (Bem), Romeno (Bem).
Fala Speaking	Português (Bem), Inglês (Bem), Romeno (Bem).
Lê Reading	Português (Bem), Inglês (Bem), Romeno (Bem).
Escreve Writing	Português (Bem), Inglês (Bem), Romeno (Bem).

Artigos em revistas com arbitragem científica Papers in periodics with scientific refereeing

1.	Zatloukalová, Martina; Enache, Teodor A; Kren, Vladimír; Ulrichová, Jitka; Vacek, Jan; Oliveira-Brett, Ana M. 2013. " Effect of 3- O -Galloyl Substitution on the Electrochemical Oxidation of Quercetin and Silybin Galloyl Esters at Glassy Carbon Electrode ", Electroanalysis 25, 7: 1621 - 1627.

2.	de Souza G. E; Adrian Enache, T; Maria Oliveira-Brett, A. 2013. "Redox Behaviour of Verbascoside and Rosmarinic Acid", Combinatorial Chemistry & High Throughput Screening 16, 2: 92 - 97.

3.	Enache, Teodor A; Amine, Aziz; Brett, Christopher M; Oliveira-Brett, Ana M. 2013. "Virgin olive oil ortho-phenols—electroanalytical quantification", Talanta 105, 1: 179 - 186.

4.	Enache, T.A.; Oliveira-Brett, A.M.. 2013. "Peptide methionine sulfoxide reductase A (MsrA): Direct electrochemical oxidation on carbon electrodes", Bioelectrochemistry 89, 1: 11 - 18.

5.	Oliveira, S.C.B.; Santarino, I.B.; Enache, T.A.; Nunes, C.; Laranjinha, J.; Barbosa, R.M.; Oliveira-Brett, A.M.. 2013. "Human colon adenocarcinoma HT-29 cell: Electrochemistry and nicotine stimulation", Bioelectrochemistry 94, 1: 30 - 38.

6.	de Souza G. E; Enache, Adrian T; de Oliveira-Brett, A. M. 2012. "Anodic Behaviour of Flavonoids Orientin, Eriodictyol and Robinin at a Glassy Carbon Electrode", Electroanalysis 24, 7: 1576 - 1583.

7.	Golea, Diana; Diculescu, Victor C; Enache, Teodor A; Butu, Alina; Tugulea, Laura; de Oliveira-Brett, A. M. 2012. "ELECTROCHEMICAL EVALUATION OF dsDNA - LIPOSOMES INTERACTIONS ", Journal of Nanomaterials and Biostructures 7, 3: 1333 - 1342.

8.	Enache, Teodor A; Oliveira Brett, A. M. 2011. "Boron doped diamond and glassy carbon electrodes comparative study of the oxidation behaviour of cysteine and methionine", Bioelectrochemistry 81, 1: 46 - 52. The electrochemical oxidation behaviour at boron doped diamond and glassy carbon electrodes of the sulphur-containing amino acids cysteine and methionine, using cyclic and differential pulse voltammetry over a wide pH range, was compared. The oxidation reactions of these amino acids are irreversible, diffusion-controlled pH dependent processes, and occur in a complex cascade mechanism. The amino acid cysteine undergoes similar three consecutive oxidation reactions at both electrodes. The first step involves the oxidation of the sulfhydryl group with radical formation, that undergoes nucleophilic attack by water to give an intermediate species that is oxidized in the second step to cysteic acid. The oxidation of the sulfhydryl group leads to a disulfide bridge between two similar cysteine moieties forming cysteine. The subsequent oxidation of cystine occurs at a higher potential, due to the strong disulfide bridge covalent bond. The electro-oxidation of methionine at a glassy carbon electrode occurs in two steps, corresponding to the formation of sulfoxide and sulfone, involving the adsorption and protonation/deprotonation of the thiol group, followed by electrochemical oxidation. Methionine undergoes a one-step oxidation reaction at boron doped diamond electrodes due to the negligible adsorption, and the oxidation also leads to the formation of methionine sulfone.

9.	Enache, Teodor A; Oliveira Brett, A. M. 2011. "Phenol and para-substituted phenols electrochemical oxidation pathways", Journal of Electroanalytical Chemistry 655, 1: 9 - 16. The electrochemical behaviour of phenol, catechol, hydroquinone, resorcinol, dopamine, and para-substituted phenolic compounds, 4-ethylphenol, tyrosine, and tyramine, was studied over a wide pH range using a glassy carbon electrode. The oxidation of phenol is pH dependent and irreversible, occurring in one step, and followed by hydrolyse in ortho- and para-positions, leading to two oxidation products, catechol and hydroquinone. The oxidation of phenol oxidation products, ortho-phenol and para-phenol, is reversible and pH dependent. The oxidation potential of para-substituted phenols varies slightly due to their substituent group in position C4, and occurs in one oxidation step corresponding to the oxidation of phenol. The oxidation products of this group of para-substituted phenols are reversibly oxidised and adsorb on the electrode surface.

10.	Enache, Teodor A; Oliveira Brett, A. M. 2011. "Pathways of Electrochemical Oxidation of Indolic Compounds", Electroanalysis 23, 6: 1337 - 1344. The electrochemical behaviour of indole and a group of indole-containing compounds with a substituent at the C3 position, indol-3-acetamide (IAM), tryptamine, gramine, indole acetic acid (IAA), indole propionic acid (IPA), indole butyric acid (IBA) and tryptophan, was investigated at a glassy carbon electrode, in order to determine their oxidation pathways. Indole undergoes one irreversible pH dependent oxidation, whereas the oxidation process of indole derivatives was more complex, a two step, the oxidation at C2 position on the pyrrole ring followed by the hydroxylation at the C7 position of the benzene moiety of indoles, irreversible pH dependent oxidation.

11.	Enache, Teodor A; Fatibello-Filho, Orlando; Oliveira Brett, A. M. 2010. "Electrochemical behavior of triflusal, aspirin and their metabolites at glassy carbon and boron doped diamond electrodes", Combinatorial Chemistry & High Throughput Screening 13, 7: 569 - 577. The electrochemical behavior of triflusal (TRF) and aspirin (ASA), before and after hydrolysis in water and in alkaline medium using two different electrode surfaces, glassy carbon and boron doped diamond, was studied by differential pulse voltammetry over a wide pH range. The hydrolysis products were 2-(hydroxyl)-4-(trifluoromethyl)- benzoic acid (HTB) for triflusal and salicylic acid (SA) for aspirin, which in vivo represent their main metabolites. The hydrolysis processes were also followed by spectrophotometry. The UV results showed complete hydrolysis after one hour for TRF and after two hours for ASA in alkaline solution. The glassy carbon electrode enables only indirect determination of TRF and ASA through the electrochemical detection of their hydrolysis products HTB and SA, respectively. The oxidation processes of HTB and SA are pH dependent and involve different numbers of electrons and protons. Moreover, the difference between the oxidation peak potential of SA and HTB was equal to 100 mV in the studied pH range from 1 to 8 due to the CF3 of the aromatic ring of HTB molecule. Due to its wider oxidation potential range, the boron doped diamond electrode was used to study the direct oxidation of TRF and ASA, as well as of their respective metabolites HTB and SA.

12.	Diculescu, Victor C; Enache, Teodor A; Oliveira, Paulo J; Oliveira Brett, A. M. 2009. "Electrochemical Oxidation of Berberine and of Its Oxidation Products at a Glassy Carbon Electrode", Electroanalysis 21, 9: 1027 - 1034. The electrochemical behavior of berberine, an isoquinoline plant alkaloid with a wide spectrum of physiological effects, was studied at a glassy carbon electrode using cyclic, differential pulse and square-wave voltammetry. The oxidation of berberine is a quasireversible, diffusion-controlled process and occurred in a cascade mechanism with the formation of several oxidation products. The diffusion coefficient of berberine was calculated from cyclic voltammetry studies to be D=1.69×10-6 cm2 s-1. The oxidation process of berberine is also pH dependent and the number of electrons and protons transferred was determined using differential pulse voltammetry. The formation of several oxidation products that adsorbed at the glassy carbon electrode surface was observed and their electrochemical behavior characterized. A mechanism for the oxidation of berberine at a glassy carbon electrode was proposed.

13.	Diculescu, Victor C; Enache, Teodor A; Oliveira, Paulo J; Oliveira Brett, A. M. 2009. "Electrochemical Oxidation of Sanguinarine and of Its Oxidation Products at a Glassy Carbon Electrode â¿¿ Relevance to Intracellular Effects", Electroanalysis 22, 1: 113 - 120. The electrochemical behavior of sanguinarine, a quaternary benzophenanthridine glycoside alkaloid with antimicrobial, anti-inflammatory, antioxidant and/or immune-stimulatory activities, was studied at a glassy carbon electrode using cyclic, differential pulse, and square wave voltammetry. The oxidation of sanguinarine is a quasireversible, diffusion-controlled process and occurred in a cascade mechanism with the formation of several oxidation products which adsorbed at the electrode surface. The oxidation of sanguinarine is pH dependent and involves the transfer of the same number of electrons and protons. The adsorbed sanguinarine oxidation products are reversibly oxidized at the glassy carbon electrode surface and their oxidation for a wide range of pHs was also studied by differential pulse and square wave voltammetry. A mechanism for the oxidation of sanguinarine at glassy carbon electrode is proposed.

14.	Enache, Teodor A; Chiorcea-Paquim, Ana-Maria; Fatibello-Filho, Orlando; Oliveira Brett, A. M. 2009. "Hydroxyl radicals electrochemically generated in situ on a boron-doped diamond electrode", Electrochemistry Communications 11, 7: 1342 - 1345. The hydroxyl radicals electrochemically generated in situ on a boron-doped diamond (BDD) electrode have been investigated for the first time in different electrolyte media, over the whole pH range between 1 and 11. A more extensive characterisation of BDD electrochemical properties is very important to understand the reactivity of organic compounds towards electrochemical oxidation on the BDD electrode, which is related to their interaction with adsorbed hydroxyl radicals due to water oxidation on the electrode surface. An oxidation peak corresponding to the transfer of one electron and one proton was observed in pH <9 electrolytes, associated with the water discharge process and electrochemical generation of hydroxyl radicals, which can interact and enhance the electro-oxidation of organic compounds. In pH >9 electrolytes the electrochemical generation of hydroxyl radicals was not observed; ammonia buffer electrolyte gave a pH-independent peak corresponding to the ammonia oxidation reaction. Additionally, for most pH values studied, a few small peaks associated with the electrochemical interaction between non-diamond carbon species on the doped diamond electrode surface and the electrolyte were also seen, which suggests that the doped diamond is relatively unreactive, but not completely inert, and the electrogenerated hydroxyl radicals play a role as mediator in the oxidation of organics.

15.	Diculescu, Victor C; Enache, Teodor A; Oliveira Brett, A. M. 2007. "Electrochemical oxidation at a glassy carbon electrode of the anti-arrhythmia drug disopyramide", Analytical Letters 40, 15: 2860 - 2871. A voltammetric study of the oxidation of disopyramide has been carried out using a glassy carbon electrode. The electrochemical oxidation of disopyramide was investigated by cyclic, differential pulse, and square wave voltammetry. The oxidation of disopyramide is an irreversible, diffusion-controlled process. The diffusion coefficient of disopyramide was calculated in pH 7.0 phosphate buffer to be D disopyramide=3.8×10-6 cm2 s-1. The oxidation of disopyramide is also pH dependent and for electrolytes with pH between 4 and 7 occurs with the transfer of one electron and one proton. In alkaline electrolytes, two consecutive charge transfer reactions are observed: both oxidation reactions involve the transfer of two electrons but only the first also involves the transfer of two protons. Two procedures for the analytical determination of disopyramide in pH 7.0 phosphate buffer were developed and compared and a detection limit LOD=1.27 µM was obtained.

Participação em eventos Event participation

Participação em eventos Event participation

Outro tipo de participação Other kind of participation

1.	Peptide methionine sulfoxide reductase A electrochemical oxidation, 2011 (Conferência). Nome do evento: XXI International Symposium on Bioelectrochemistry and Bioenergetics; Nome da Instituição: BES-2011; Cidade do evento: Cracow / Poland.

2.	Cysteine and methionine electrochemical oxidation at glassy carbon and boron doped diamond electrodes, 2010 (Conferência). Nome do evento: 61st Annual Meeting of International Society of Electrochemistry; Nome da Instituição: ISE; Cidade do evento: Nisa / França.

3.	Comportamento electroquímico do fenol compostos fenólicos, 2010 (Conferência). Nome do evento: XIX Congresso da Sociedade Iberoamericana de Electroquímica; Nome da Instituição: SIBAE; Cidade do evento: Alcal'a / Espanha.

4.	Electrochemical oxidation pathways of indolic compounds, 2010 (Conferência). Nome do evento: 13th International conference of electroanalysis; Nome da Instituição: ESEAC; Cidade do evento: Gijón / Espanha.

5.	Development and characterization of guanine-reach DNA modified surfaces, 2010 (Conferência). Nome do evento: Analytical and Nanoanalytical Methods for Biomedical and Environmental Sciences "IC-ANMBES-2010"; Nome da Instituição: "IC-ANMBES-2010"; Cidade do evento: Brasov / Roménia.

6.	Electrochemical Studies of DNA-Liposome Complexes Formation, 2010 (Conferência). Nome do evento: Analytical and Nanoanalytical Methods for Biomedical and Environmental Sciences "IC-ANMBES-2010; Nome da Instituição: "IC-ANMBES" 2010; Cidade do evento: Brasov / Roménia.

7.	Hydroxyl radicals electrochemically generated in situ on a boron-doped diamond electrode, 2009 (Conferência). Nome do evento: http://www.je09.org/; Nome da Instituição: JE09; Cidade do evento: Sinaia / Roménia.

8.	Electrochemical behavior of aspirin, triflusal and theirs metabolites at glassy carbon and boron doped diamond electrodes, 2009 (Conferência). Nome do evento: XX International Symposium on Bioelectrochemistry and Bioenergetics; Nome da Instituição: BES; Cidade do evento: Sibiu / Roménia.

9.	Electrochemical Oxidation of Some Isoquinoline Alkaloids, 2008 (Conferência). Nome do evento: 59th Annual Meeting of the International Society of Electrochemistry; Nome da Instituição: ISE; Cidade do evento: Seville / Espanha.

10.	Electrochemical oxidation of anti-arrhythmia drug disopyramide at a glassy carbon electrode , 2008 (Conferência). Nome do evento: 59th Annual Meeting of the International Society of Electrochemistry; Nome da Instituição: ISE; Cidade do evento: Seville / Espanha.

11.	Nanobioelectroquimica: Caracterização de Eléctrodos Modificados e Aplicações, 2008 (Conferência). Nome do evento: XVIII Congreso de la Sociedad Iberoamericana de Electroquimica; Nome da Instituição: SIBAE-2008; Cidade do evento: Medelin / Colombia.

Produção científica Scientific production

Total

Produção científica Scientific production

15

Artigos científicos em revistas Papers in periodics

15

Com arbitragem científica With scientific refereeing

15

	Ana-Maria Chiorcea Paquim
	Victor Constantin Diculescu