Joana Sá Ramalho Simões. É da Universidade de Aveiro. Publicou 7 artigos em revistas especializadas e 17 trabalhos em actas de eventos. Possui 10 itens de produção técnica. Actua na área de Ciências Exactas com ênfase em Química. Nas suas actividades profissionais interagiu com 14 colaboradores em co-autorias de trabalhos científicos. No seu curriculum DeGóis os termos mais frequentes na contextualização da produção científica, tecnológica e artístico-cultural são: Espectrometria de massa, Galactomananas, Resíduo de café, Aloé vera, Actividade imunoestimuladora, Bebida de Café, Oligossacarídeos, Goma de alfarroba, Acetilação de Polissacarídeos e Configuração anomérica.
Endereço de acesso a este CV: http://www.degois.pt/visualizador/curriculum.jsp?key=4917292228646521

Nome completo Full name	Joana Sá Ramalho Simões
Nome em citações bibliográficas Quoting name	Simões, Joana
Domínio científico de atuação Scientific domain	Ciências Exactas-Química.

Endereço profissional Professional address

Universidade de Aveiro Departamento de Química Química Orgânica, Produtos Naturais e Agro-Alimentares - QOPNAA Campus Universitário de Santiago Aveiro 3810-193 Aveiro Portugal Correio electrónico: joana.simoes@ua.pt

Sexo Gender

Feminino»Female

2007-2012	Doutoramento Phd
Doutoramento em Química. Universidade de Aveiro, Portugal.

2004-2007	Mestrado Master degree
Mestrado em Química e Qualidade dos Alimentos (2 anos » years) . Universidade de Aveiro, Portugal.

1999-2004	Licenciatura Licentiate degree
Bioquímica e Química Alimentar (5 anos » years) . Universidade de Aveiro, Portugal.

Universidade de Aveiro

Jun/2007-Mai/2011

Outra Situação

Universidade de Aveiro

Jul/2006-Mai/2007

Outra Situação

Universidade de Aveiro

Set/2005-Jun/2006

Outra Situação

Nestlé Portugal, S.A. - Fábrica de Avanca

Set/2003-Jul/2004

Estagiário

Universidade de Aveiro

Jan/2002-Jul/2003

Outra Situação

Universidade de Aveiro

Jun/2007-Mai/2011

Universidade de Aveiro

Set/2005-Jun/2006	Departamento de Química

Nestlé Portugal, S.A. - Fábrica de Avanca

Set/2003-Jul/2004

Nestlé Portugal, S.A. - Fábrica de Avanca

Set/2003-Jul / 2004

Universidade de Aveiro

Jan/2002-Jul/2003
Atividade realizada»Activity:

Compreende Understandig	Português (Bem), Inglês (Bem), Espanhol (Razoavelmente).
Fala Speaking	Português (Bem), Inglês (Bem), Espanhol (Pouco).
Lê Reading	Português (Bem), Inglês (Bem), Espanhol (Razoavelmente).
Escreve Writing	Português (Bem), Inglês (Bem), Espanhol (Pouco).

Capítulos de livros publicados Published book chapters

1.	Simões, Joana; Coimbra, Manuel A; Domingues, M. R; Nunes, Fernando M; Madureira, Pedro; Vilanova, Manuel. 2007. Estudo das mananas do café como fonte de compostos bioactivos.  In 4ª Jornada Bebidas e Saúde, iBeSa- Instituto de Bebidas e Saúde, 281 - 293. ISBN: 978-972-9993. Porto: Mediadigital Lda.

Artigos em revistas com arbitragem científica Papers in periodics with scientific refereeing

1.	Simões, Joana; Nunes, Fernando M; Domingues, Pedro; Coimbra, Manuel A; Domingues, M. R. 2012. "Mass spectrometry characterization of an Aloe vera mannan presenting immunostimulatory activity", Carbohydrate Polymers, in press: 0 - 0.

2.	Cerqueira, Miguel A; Souza, Bartolomeu W; Simões, Joana; Teixeira, José A; Domingues, M. R; Coimbra, Manuel A; Vicente, António A. 2011. "Structural and thermal characterization of galactomannans from non-conventional sources", Carbohydrate Polymers 83, 1: 179 - 185. Galactomannans of Gleditsia triacanthos, Caesalpinia pulcherrima and Adenanthera pavonina were structurally and thermally characterized. Methylation analyses confirmed that these three galactomannans are composed by a 4-linked mannose polymer with galactose side chains attached at the C6 position. G. triacanthos galactomannan had an estimated average degree of polymerization of 224 mannose residues, with a degree of branching of 0.24, C. pulcherrima galactomannan had a degree of polymerization of 252 and a degree of branching of 0.30, and A. pavonina galactomannan had a degree of polymerization of 475 and a degree of branching of 0.60. Enzymatic hydrolysis with endo-ß-mannanase and the subsequent analyses of the low molecular weight fraction by ESI-MS/MS indicated the presence of characteristic structural features of galactomannans. Furthermore, acetyl and pentosyl residues were detected in the galactomannan of G. triacanthos and analyses of higher molecular weight fractions from G. triacanthos were performed, confirming that presence. Differential scanning calorimetry (DSC) showed the presence of two peaks related with water loss, confirmed by thermogravimetric analysis (TGA). The different compositions of galactomannans with different mannose content and different molecular weights influence their thermal behaviour. A higher content of mannose lead to higher values of the enthalpy change (first thermal transition) and glass transition temperature (Tg), while the enthalpy change of the second thermal transition was influenced by the viscosity average molecular weight (Mv) of the samples. This work provides understanding of structural and thermal properties of three galactomannans from non-conventional sources, thus contributing to a better insight on their possible food, pharmaceutical or biomedical applications.

3.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2011. "Demonstration of the presence of acetylation and arabinose branching as structural features of locust bean gum galactomannans ", Carbohydrate Polymers , 86: 1476 - 1483. Galactomannans from locust bean gum (LBG) are polysaccharides containing single Galp residues as side chains in a ß-(1¿4)-Manp backbone. Nevertheless, sugars other than mannose and galactose are always detected, namely, arabinose and glucose, although in small amounts. In order to demonstrate that LBG galactomannans contain other structural features than those usually reported in literature, the galactomannans from LBG obtained from two different commercial sources were submitted to a selective degradation by Aspergillus niger endo-ß-d-mannanase and the oligosaccharides (OS) obtained were fractioned by size exclusion chromatography using a Biogel-P2 (100-1800 Da). A. niger endo-ß-d-mannanase is able to hydrolyse the ß-(1¿4)-linked mannan backbone when the mannose residues are not hindered by the presence of substituents. This enables collection of fragments of the substituted mannan polysaccharide in the different chromatographic fractions that can be analysed by Electrospray Tandem Mass Spectrometry (ESI-MSn). Both samples showed the presence of hexose OS (Hex3-6), acetylated hexoses (AcHex3-6), hexoses substituted with a pentose unit (PentHex3-6), and OS composed by acetylated hexoses substituted with a pentose unit (PentAcHex3-6). These structures were confirmed by GC-qMS of reduced and permethylated oligosaccharides. Sugar and glycosidic-linkage analysis allowed to assign the pentose unit to a terminally-linked arabinofuranose residue at the O-6 of a (1¿4)-Manp residue. The presence of arabinose substituted OS and acetylated mannoses have never been reported to occur in LBG. .

4.	Moreira, A. S. P; Coimbra, Manuel A; Nunes, Fernando M; Simões, Joana; Domingues, M. R. 2011. "Evaluation of the Effect of Roasting on the Structure of Coffee Galactomannans Using Model Oligosaccharides", Journal of Agricultural and Food Chemistry, 59 (18): 10078 - 11087.

5.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2010. "Structural features of partially acetylated coffee galactomannans presenting immunostimulatory activity", Carbohydrate Polymers 79, 2: 397 - 402. The galactomannans purified from coffee infusions have been shown to present in vitro immunostimulatory activity on murine B- and T-lymphocytes. These properties have also been shown characteristic of the galactomannans recovered from coffee residue by strong alkali solutions and rendered soluble in water by partial acetylation. In this study, purified fractions of galactomannans with immunostimulatory activity obtained from coffee infusion and from coffee residue were compared according to their average molecular weight determined by size-exclusion chromatography on Sephacryl S300, glycosidic-linkage composition by methylation analysis, selective hydrolysis by an endo-(1 ¿ 4)-ß-d-mannanase, analysis of the resultant oligosaccharide profile by Bio-Gel P2 separation, and determination of the pattern of acetylation by electrospray tandem mass spectrometry (ESI-MS/MS). It was found that the galactomannans have a comparable molecular weight (90–110 kDa), and similar glycosidic-linkage composition. However, the galactomannans from coffee residue were preferentially acetylated in the side chain residues whereas the galactomannans recovered from coffee infusions only had acetyl groups directly linked to the backbone residues. These results show that these polysaccharides that present comparable immunostimulatory properties have different acetylation patterns.

6.	Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Domingues, M. R; Vilanova, Manuel; Coimbra, Manuel A. 2009. "Immunostimulatory properties of coffee mannans", Molecular Nutrition & Food Research 53, 8: 1036 - 1043. Coffee infusion mannans are acetylated polysaccharides containing single Galp and Araf residues as side chains of a beta-(1 --> 4)-Manp backbone. These mannans are structurally similar to the bioactive acetylated mannans from Aloe vera (AV). In this study, acetylated mannans were obtained from two coffee infusions prepared from light and dark roasted beans. These samples were tested for their immunostimulatory activity and compared with an extract of AV mannan and with locust bean gum (LBG) galactomannans. The coffee samples, as well as the AV extract, stimulated murine B- and T-lymphocytes, as evaluated by the in vitro expression of the surface lymphocyte activation marker CD69, more marked on B- than on T-lymphocytes. In coffee samples, contrarily to the AV, no proliferative effect was noticed. LBG sample did not show any immunostimulatory activity. Because the material that remains in the residue of the hot water extraction was still very rich in mannans, a sequential extraction was performed and a main fraction was recovered with a 4 M NaOH solution. Because this material was insoluble in water, a partial acetylation was performed. These polysaccharides also showed immunostimulatory activity, opening the possibility of exploitation of coffee infusion and coffee residue as sources of bioactive polysaccharides.

7.	Simões, Joana; Domingues, Pedro; Reis, Ana; Nunes, Fernando M; Coimbra, Manuel A; Domingues, M. R. 2007. "Identification of Anomeric Configuration of Underivatized Reducing Glucopyranosyl-glucose Disaccharides by Tandem Mass Spectrometry and Multivariate Analysis", Analytical Chemistry 79, 15: 5896 - 5905. The possibility of discrimination of the anomeric configuration (a or ß) of underivatized reducing glucopyranosyl-glucose disaccharides, using a hybrid mass spectrometer Q-TOF 2 (Micromass), a linear ion trap LXQ (Thermo), and a triple quadrupole Quattro (Micromass) with an electrospray source (ESI) was investigated. Differences observed in the relative abundances of specific product ions obtained from collisionally induced dissociation of the [M + Li]+ adducts were statistically analyzed, and discriminant analysis was performed. MANOVA has shown that anomeric configuration has influence on the combined dependent variables (relative abundances of m/z product ions) in all the three mass spectrometers used (Q-TOF 2, LIT, and QqQ). Discriminant analysis has shown that, in all instruments, it is possible to discriminate anomeric configurations and to build a diagnostic model. These diagnostic differences are even more relevant considering that no derivatization procedures are needed for obtaining this structural information. The Q-TOF 2 instrument has been shown to give data that allowed us to build a model with better discriminant power (Wilks' ¿ value of 0.014) followed by the QqQ instrument (Wilks' ¿ value of 0.029) and the LIT instrument (Wilks' ¿ value of 0.037).

Trabalhos completos/resumidos em eventos com arbitragem científica Papers in conference proceedings with scientific refereeing

1.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2011. "Enzymatically assays for structural study of coffee galactomannans", Trabalho apresentado em 9th Carbohydrate Bioengineering Meeting, In Carbohydrate Bioengineering Meeting, Lisboa.

2.	Passos, C. P; Simões, Joana; Reis, S. A; Coimbra, Manuel A. 2011. "Metodologies for the extraction of polysaccharides from spent coffee residue - a comparative study", Trabalho apresentado em 9th Carbohydrate Bioengineering Meeting, In Carbohydrate Bioengineering Meeting, Lisboa.

3.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2011. "Stability upon roasting of coffee galactomannans and arabinogalactans", Trabalho apresentado em First International Congress on Cocoa Coffee and Tea, In First International Congress on Cocoa Coffee and Tea, Novara.

4.	Coimbra, Manuel A; Simões, Joana; Passos, C. P; Domingues, M. R; Nunes, Fernando M. 2010. "Coffee polysaccharides as a source of food ingredients with biological activity", Trabalho apresentado em 25th International Carbohydrate Symposium, In International Carbohydrate Symposium, Tokyo.

5.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2009. "Increase on extractability of immunostimulatory mannans from coffee residue by a roasted procedure", Trabalho apresentado em COST 927, In Thermally processed foods: possible health implications, Aveiro.

6.	Coimbra, Manuel A; Simões, Joana; Reis, S. A; Domingues, M. R; Nunes, Fernando M. 2009. "Utilização do resíduo de café como fonte de ingredientes alimentares com actividade imunoestimuladora", Trabalho apresentado em 9º Encontro de Química dos Alimentos, In Encontro de Química dos Alimentos, Angra do Heroísmo.

7.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2009. "Chemical characterization of coffee residue mannans as affected by roasting", Trabalho apresentado em 8th International Meeting of the Portuguese Carbohydrate Group, In International Meeting of the Portuguese Carbohydrate Group, Braga.

8.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2008. "Naturally and Chemically Acetylation of Immunostimulatory Coffee Mannans", Trabalho apresentado em XXIV International Carbohydrate Symposium, In Polysaccharides- structure, function , applications, Oslo.

9.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2008. "Structural features between mannans with and without imunostimulatory activities", Trabalho apresentado em IV Iberian Carbohydrate Meeting, In Iberian Carbohydrate Meeting, Santiago de Compostela.

10.	Coimbra, Manuel A; Simões, Joana; Domingues, M. R; Madureira, Pedro; Vilanova, Manuel; Nunes, Fernando M. 2008. "Immunostimulatory properties of coffee mannans", Trabalho apresentado em 22nd International Conference on Coffee Science – ASIC, In International Conference on Coffee Science, São Paulo.

11.	Domingues, M. R; Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Vilanova, Manuel; Coimbra, Manuel A. 2007. "Immunmodulatory Properties obtained from Coffee Residue. A Mass Spectrometry Study", Trabalho apresentado em COST 927, In Thermally processed foods: Possible health implications, Sofia.

12.	Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Domingues, M. R; Vilanova, Manuel; Coimbra, Manuel A. 2007. "Characterization by mass spectrometry of chemically acetylated mannans obtained from coffee residue and presenting immunostimulatory activity", Trabalho apresentado em 7th International Meeting of the Portuguese Carbohydrate Group, In International Meeting of the Portuguese Carbohydrate Group, Oeiras.

13.	Domingues, M. R; Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Vilanova, Manuel; Coimbra, Manuel A. 2007. "Identification of Underivatized Reducing Glucopyranosylglucose Disaccharides Anomers by Electrospray Tandem Mass Spectrometry", Trabalho apresentado em 7th International Meeting of the Portuguese Carbohydrate Group, In International Meeting of the Portuguese Carbohydrate Group, Oeiras.

14.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2007. "Characterization by mass spectrometry of acetylated mannans of Aloe vera", Trabalho apresentado em 4th Annual meeting of the Portuguese Proteomics Network / 1st Meeting of the Portuguese National Mass Spectrometry Network- ProCura RNEM, In Annual meeting of the Portuguese Proteomics Network / Meeting of the Portuguese National Mass Spectrometry Network- ProCura RNEM, Aveiro.

15.	Simões, Joana; Coimbra, Manuel A; Domingues, M. R; Nunes, Fernando M; Madureira, Pedro; Vilanova, Manuel. 2007. "Estudo das mananas do café como fonte de compostos bioactivos", Trabalho apresentado em 4ª Jornada Bebidas e Saúde, iBeSa- Instituto de Bebidas e Saúde, In Jornada Bebidas e Saúde, iBeSa- Instituto de Bebidas e Saúde, Porto.

16.	Coimbra, Manuel A; Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Domingues, M. R; Vilanova, Manuel. 2006. "Immunomodulator Properties of Coffee Infusion Mannans", Trabalho apresentado em COST 926/927, In Molecular and physiological effects of bioactive food compounds, Viena.

17.	Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Domingues, M. R; Vilanova, Manuel; Coimbra, Manuel A. 2006. "Immunomodulator activity of coffee infusions acetylated mannans", Trabalho apresentado em XV National Congress of Biochemistry, In XVth National Congress of Biochemistry, Aveiro.

Apresentação oral de trabalho Oral work presentation

1.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. Enzymatically assays for structural study of coffee galactomannans,9th Carbohydrate Bioengineering Meeting,Lisboa,2011 (Poster).

2.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. Stability upon roasting of coffee galactomannans and arabinogalactans,First International Congress on Cocoa Coffee and Tea,Novara,2011 (Poster).

3.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. Increase on extractability of immunostimulatory mannans from coffee residue by a roasted procedure,COST 927,Aveiro,2009 (Comunicação).

4.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. Chemical characterization of coffee residue mannans as affected by roasting,8th International Meeting of the Portuguese Carbohydrate Group,Braga,2009 (Poster).

5.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. Naturally and Chemically Acetylation of Immunostimulatory Coffee Mannans,XXIV International Carbohydrate Symposium,Oslo,2008 (Poster).

6.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. Structural features between mannans with and without imunostimulatory activities,IV Iberian Carbohydrate Meeting,Santiago de Compostela,2008 (Poster).

7.	Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. Characterization by mass spectrometry of acetylated mannans of Aloe vera,4th Annual Meeting of the Portuguese Proteomics Network/ 1st Meeting of the Portuguese National Mass Spectrometry Network,Aveiro,2007 (Poster).

8.	Simões, Joana; Coimbra, Manuel A; Domingues, M. R; Nunes, Fernando M; Madureira, Pedro; Vilanova, Manuel. Estudo das mananas do café como fonte de compostos bioactivos,4ª Jornada Bebidas e Saúde, iBeSa- Instituto de Bebidas e Saúde,Porto,2007 (Comunicação).

9.

Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Domingues, M. R; Vilanova, Manuel; Coimbra, Manuel A. Characterization by mass spectrometry of chemically acetylated mannans obtained from coffee residue and presenting immunostimulatory activity,7th International Meeting of the Portuguese Carbohydrate Group,Oeiras,2007 (Poster).

10.	Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Domingues, M. R; Vilanova, Manuel; Coimbra, Manuel A. Immunomodulator activity of coffee infusions acetylated mannans,XV National Congress of Biochemistry,Aveiro,2006 (Poster).

Produção científica Scientific production

Produção técnica Technical production

Total

Produção científica Scientific production

25

Livros e capítulos Books and book chapters

1

Capítulos de livros publicados Published book chapters

1

Artigos científicos em revistas Papers in periodics

7

Com arbitragem científica With scientific refereeing

7

Trabalhos em eventos Papers in conference proceedings

17

Com arbitragem científica With scientific refereeing

17

Total

Produção técnica Technical production

10

Outros tipos de produção técnica Other technical production

10

Fernando Herminio Ferreira Milheiro Nunes