Joana Sá Ramalho Simões
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Data da última atualização
»Last update
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03/02/2015 |
Dados pessoais (Personal data)
Nome completo
Full name |
Joana Sá Ramalho Simões |
Nome em citações bibliográficas
Quoting name |
Simões, Joana |
Domínio científico de atuação
Scientific domain |
Ciências Exactas-Química.
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Endereço profissional
Professional address |
Universidade de Aveiro Departamento de Química Química Orgânica, Produtos Naturais e Agro-Alimentares - QOPNAA Campus Universitário de Santiago Aveiro 3810-193 Aveiro Portugal Correio electrónico: joana.simoes@ua.pt |
Sexo
Gender |
Feminino»Female |
Graus Académicos
(Academic Degrees)
2007-2012 |
Doutoramento Phd |
Doutoramento em Química.
Universidade de Aveiro,
Portugal.
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2004-2007 |
Mestrado Master degree |
Mestrado em Química e Qualidade dos Alimentos
(2 anos » years)
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Universidade de Aveiro,
Portugal.
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1999-2004 |
Licenciatura Licentiate degree |
Bioquímica e Química Alimentar
(5 anos » years)
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Universidade de Aveiro,
Portugal.
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Vínculos profissionais
(Professional Positions)
Jun/2007-Mai/2011 |
Outra Situação |
Jul/2006-Mai/2007 |
Outra Situação |
Set/2005-Jun/2006 |
Outra Situação |
Nestlé Portugal, S.A. - Fábrica de Avanca |
Set/2003-Jul/2004 |
Estagiário |
Jan/2002-Jul/2003 |
Outra Situação |
Atividades de Investigação e Desenvolvimento (Research and Development activities)
Set/2005-Jun/2006 |
Departamento de Química |
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Estágios realizados (Traineeships)
Nestlé Portugal, S.A. - Fábrica de Avanca |
Atividades de Formação Profissional (Professional education activities)
Nestlé Portugal, S.A. - Fábrica de Avanca |
Outras atividades técnico científicas (Other technical-scientific activities)
Jan/2002-Jul/2003 |
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Atividade realizada»Activity:
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Línguas (Languages)
Compreende Understandig |
Português (Bem), Inglês (Bem), Espanhol (Razoavelmente). |
Fala Speaking |
Português (Bem), Inglês (Bem), Espanhol (Pouco). |
Lê Reading |
Português (Bem), Inglês (Bem), Espanhol (Razoavelmente). |
Escreve Writing |
Português (Bem), Inglês (Bem), Espanhol (Pouco). |
Produção científica, técnica e artística/cultural
(Scientific, technical and artistical/cultural
production)
Capítulos de livros publicados Published book chapters |
1. |
Simões, Joana; Coimbra, Manuel A; Domingues, M. R; Nunes, Fernando M; Madureira, Pedro; Vilanova, Manuel. 2007. Estudo das mananas do café como fonte de compostos bioactivos. In 4ª Jornada Bebidas e Saúde, iBeSa- Instituto de Bebidas e Saúde, 281 - 293. ISBN: 978-972-9993. Porto: Mediadigital Lda. |
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Artigos em revistas com arbitragem científica Papers in periodics with scientific refereeing |
1. |
Simões, Joana; Nunes, Fernando M; Domingues, Pedro; Coimbra, Manuel A; Domingues, M. R. 2012. "Mass spectrometry characterization of an Aloe vera mannan presenting immunostimulatory activity", Carbohydrate Polymers, in press: 0 - 0.
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2. |
Cerqueira, Miguel A; Souza, Bartolomeu W; Simões, Joana; Teixeira, José A; Domingues, M. R; Coimbra, Manuel A; Vicente, António A. 2011. "Structural and thermal characterization of galactomannans from non-conventional sources", Carbohydrate Polymers 83, 1: 179 - 185. Galactomannans of Gleditsia triacanthos, Caesalpinia pulcherrima and Adenanthera pavonina were structurally and thermally
characterized. Methylation analyses confirmed that these three galactomannans are composed by a 4-linked mannose polymer with
galactose side chains attached at the C6 position. G. triacanthos galactomannan had an estimated average degree of polymerization
of 224 mannose residues, with a degree of branching of 0.24, C. pulcherrima galactomannan had a degree of polymerization of
252 and a degree of branching of 0.30, and A. pavonina galactomannan had a degree of polymerization of 475 and a degree of
branching of 0.60. Enzymatic hydrolysis with endo-ß-mannanase and the subsequent analyses of the low molecular weight fraction
by ESI-MS/MS indicated the presence of characteristic structural features of galactomannans. Furthermore, acetyl and pentosyl
residues were detected in the galactomannan of G. triacanthos and analyses of higher molecular weight fractions from G. triacanthos
were performed, confirming that presence. Differential scanning calorimetry (DSC) showed the presence of two peaks related
with water loss, confirmed by thermogravimetric analysis (TGA). The different compositions of galactomannans with different
mannose content and different molecular weights influence their thermal behaviour. A higher content of mannose lead to higher
values of the enthalpy change (first thermal transition) and glass transition temperature (Tg), while the enthalpy change
of the second thermal transition was influenced by the viscosity average molecular weight (Mv) of the samples. This work provides
understanding of structural and thermal properties of three galactomannans from non-conventional sources, thus contributing
to a better insight on their possible food, pharmaceutical or biomedical applications.
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3. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2011. "Demonstration of the presence of acetylation and arabinose branching as structural features of locust bean gum galactomannans
", Carbohydrate Polymers , 86: 1476 - 1483. Galactomannans from locust bean gum (LBG) are polysaccharides containing single Galp residues as side chains in a ß-(1¿4)-Manp
backbone. Nevertheless, sugars other than mannose and galactose are always detected, namely, arabinose and glucose, although
in small amounts. In order to demonstrate that LBG galactomannans contain other structural features than those usually reported
in literature, the galactomannans from LBG obtained from two different commercial sources were submitted to a selective degradation
by Aspergillus niger endo-ß-d-mannanase and the oligosaccharides (OS) obtained were fractioned by size exclusion chromatography
using a Biogel-P2 (100-1800 Da). A. niger endo-ß-d-mannanase is able to hydrolyse the ß-(1¿4)-linked mannan backbone when
the mannose residues are not hindered by the presence of substituents. This enables collection of fragments of the substituted
mannan polysaccharide in the different chromatographic fractions that can be analysed by Electrospray Tandem Mass Spectrometry
(ESI-MSn). Both samples showed the presence of hexose OS (Hex3-6), acetylated hexoses (AcHex3-6), hexoses substituted with
a pentose unit (PentHex3-6), and OS composed by acetylated hexoses substituted with a pentose unit (PentAcHex3-6). These structures
were confirmed by GC-qMS of reduced and permethylated oligosaccharides. Sugar and glycosidic-linkage analysis allowed to assign
the pentose unit to a terminally-linked arabinofuranose residue at the O-6 of a (1¿4)-Manp residue. The presence of arabinose
substituted OS and acetylated mannoses have never been reported to occur in LBG.
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4. |
Moreira, A. S. P; Coimbra, Manuel A; Nunes, Fernando M; Simões, Joana; Domingues, M. R. 2011. "Evaluation of the Effect of Roasting on the Structure of Coffee Galactomannans Using Model Oligosaccharides", Journal of Agricultural and Food Chemistry, 59 (18): 10078 - 11087.
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5. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2010. "Structural features of partially acetylated coffee galactomannans presenting immunostimulatory activity", Carbohydrate Polymers 79, 2: 397 - 402. The galactomannans purified from coffee infusions have been shown to present in vitro immunostimulatory activity on murine
B- and T-lymphocytes. These properties have also been shown characteristic of the galactomannans recovered from coffee residue
by strong alkali solutions and rendered soluble in water by partial acetylation. In this study, purified fractions of galactomannans
with immunostimulatory activity obtained from coffee infusion and from coffee residue were compared according to their average
molecular weight determined by size-exclusion chromatography on Sephacryl S300, glycosidic-linkage composition by methylation
analysis, selective hydrolysis by an endo-(1 ¿ 4)-ß-d-mannanase, analysis of the resultant oligosaccharide profile by Bio-Gel
P2 separation, and determination of the pattern of acetylation by electrospray tandem mass spectrometry (ESI-MS/MS). It was
found that the galactomannans have a comparable molecular weight (90–110 kDa), and similar glycosidic-linkage composition.
However, the galactomannans from coffee residue were preferentially acetylated in the side chain residues whereas the galactomannans
recovered from coffee infusions only had acetyl groups directly linked to the backbone residues. These results show that these
polysaccharides that present comparable immunostimulatory properties have different acetylation patterns.
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6. |
Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Domingues, M. R; Vilanova, Manuel; Coimbra, Manuel A. 2009. "Immunostimulatory properties of coffee mannans", Molecular Nutrition & Food Research 53, 8: 1036 - 1043. Coffee infusion mannans are acetylated polysaccharides containing single Galp and Araf residues as side chains of a beta-(1
--> 4)-Manp backbone. These mannans are structurally similar to the bioactive acetylated mannans from Aloe vera (AV). In this
study, acetylated mannans were obtained from two coffee infusions prepared from light and dark roasted beans. These samples
were tested for their immunostimulatory activity and compared with an extract of AV mannan and with locust bean gum (LBG)
galactomannans. The coffee samples, as well as the AV extract, stimulated murine B- and T-lymphocytes, as evaluated by the
in vitro expression of the surface lymphocyte activation marker CD69, more marked on B- than on T-lymphocytes. In coffee samples,
contrarily to the AV, no proliferative effect was noticed. LBG sample did not show any immunostimulatory activity. Because
the material that remains in the residue of the hot water extraction was still very rich in mannans, a sequential extraction
was performed and a main fraction was recovered with a 4 M NaOH solution. Because this material was insoluble in water, a
partial acetylation was performed. These polysaccharides also showed immunostimulatory activity, opening the possibility of
exploitation of coffee infusion and coffee residue as sources of bioactive polysaccharides.
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7. |
Simões, Joana; Domingues, Pedro; Reis, Ana; Nunes, Fernando M; Coimbra, Manuel A; Domingues, M. R. 2007. "Identification of Anomeric Configuration of Underivatized Reducing Glucopyranosyl-glucose Disaccharides by Tandem Mass Spectrometry
and Multivariate Analysis", Analytical Chemistry 79, 15: 5896 - 5905. The possibility of discrimination of the anomeric configuration (a or ß) of underivatized reducing glucopyranosyl-glucose
disaccharides, using a hybrid mass spectrometer Q-TOF 2 (Micromass), a linear ion trap LXQ (Thermo), and a triple quadrupole
Quattro (Micromass) with an electrospray source (ESI) was investigated. Differences observed in the relative abundances of
specific product ions obtained from collisionally induced dissociation of the [M + Li]+ adducts were statistically analyzed,
and discriminant analysis was performed. MANOVA has shown that anomeric configuration has influence on the combined dependent
variables (relative abundances of m/z product ions) in all the three mass spectrometers used (Q-TOF 2, LIT, and QqQ). Discriminant
analysis has shown that, in all instruments, it is possible to discriminate anomeric configurations and to build a diagnostic
model. These diagnostic differences are even more relevant considering that no derivatization procedures are needed for obtaining
this structural information. The Q-TOF 2 instrument has been shown to give data that allowed us to build a model with better
discriminant power (Wilks' ¿ value of 0.014) followed by the QqQ instrument (Wilks' ¿ value of 0.029) and the LIT instrument
(Wilks' ¿ value of 0.037).
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Trabalhos completos/resumidos em eventos com arbitragem científica Papers in conference proceedings with scientific refereeing |
1. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2011. "Enzymatically assays for structural study of coffee galactomannans", Trabalho apresentado em 9th Carbohydrate Bioengineering Meeting, In Carbohydrate Bioengineering Meeting, Lisboa. |
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2. |
Passos, C. P; Simões, Joana; Reis, S. A; Coimbra, Manuel A. 2011. "Metodologies for the extraction of polysaccharides from spent coffee residue - a comparative study", Trabalho apresentado em 9th Carbohydrate Bioengineering Meeting, In Carbohydrate Bioengineering Meeting, Lisboa. |
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3. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2011. "Stability upon roasting of coffee galactomannans and arabinogalactans", Trabalho apresentado em First International Congress on Cocoa Coffee and Tea, In First International Congress on Cocoa Coffee and Tea, Novara. |
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4. |
Coimbra, Manuel A; Simões, Joana; Passos, C. P; Domingues, M. R; Nunes, Fernando M. 2010. "Coffee polysaccharides as a source of food ingredients with biological activity", Trabalho apresentado em 25th International Carbohydrate Symposium, In International Carbohydrate Symposium, Tokyo. |
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5. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2009. "Increase on extractability of immunostimulatory mannans from coffee residue by a roasted procedure", Trabalho apresentado em COST 927, In Thermally processed foods: possible health implications, Aveiro. |
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6. |
Coimbra, Manuel A; Simões, Joana; Reis, S. A; Domingues, M. R; Nunes, Fernando M. 2009. "Utilização do resíduo de café como fonte de ingredientes alimentares com actividade imunoestimuladora", Trabalho apresentado em 9º Encontro de Química dos Alimentos, In Encontro de Química dos Alimentos, Angra do Heroísmo. |
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7. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2009. "Chemical characterization of coffee residue mannans as affected by roasting", Trabalho apresentado em 8th International Meeting of the Portuguese Carbohydrate Group, In International Meeting of the Portuguese Carbohydrate Group, Braga. |
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8. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2008. "Naturally and Chemically Acetylation of Immunostimulatory Coffee Mannans", Trabalho apresentado em XXIV International Carbohydrate Symposium, In Polysaccharides- structure, function , applications, Oslo. |
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9. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2008. "Structural features between mannans with and without imunostimulatory activities", Trabalho apresentado em IV Iberian Carbohydrate Meeting, In Iberian Carbohydrate Meeting, Santiago de Compostela. |
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10. |
Coimbra, Manuel A; Simões, Joana; Domingues, M. R; Madureira, Pedro; Vilanova, Manuel; Nunes, Fernando M. 2008. "Immunostimulatory properties of coffee mannans", Trabalho apresentado em 22nd International Conference on Coffee Science – ASIC, In International Conference on Coffee Science, São Paulo. |
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11. |
Domingues, M. R; Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Vilanova, Manuel; Coimbra, Manuel A. 2007. "Immunmodulatory Properties obtained from Coffee Residue. A Mass Spectrometry Study", Trabalho apresentado em COST 927, In Thermally processed foods: Possible health implications, Sofia. |
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12. |
Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Domingues, M. R; Vilanova, Manuel; Coimbra, Manuel A. 2007. "Characterization by mass spectrometry of chemically acetylated mannans obtained from coffee residue and presenting immunostimulatory
activity", Trabalho apresentado em 7th International Meeting of the Portuguese Carbohydrate Group, In International Meeting of the Portuguese Carbohydrate Group, Oeiras. |
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13. |
Domingues, M. R; Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Vilanova, Manuel; Coimbra, Manuel A. 2007. "Identification of Underivatized Reducing Glucopyranosylglucose Disaccharides Anomers by Electrospray Tandem Mass Spectrometry", Trabalho apresentado em 7th International Meeting of the Portuguese Carbohydrate Group, In International Meeting of the Portuguese Carbohydrate Group, Oeiras. |
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14. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. 2007. "Characterization by mass spectrometry of acetylated mannans of Aloe vera", Trabalho apresentado em 4th Annual meeting of the Portuguese Proteomics Network / 1st Meeting of the Portuguese National Mass Spectrometry Network-
ProCura RNEM, In Annual meeting of the Portuguese Proteomics Network / Meeting of the Portuguese National Mass Spectrometry Network- ProCura
RNEM, Aveiro. |
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15. |
Simões, Joana; Coimbra, Manuel A; Domingues, M. R; Nunes, Fernando M; Madureira, Pedro; Vilanova, Manuel. 2007. "Estudo das mananas do café como fonte de compostos bioactivos", Trabalho apresentado em 4ª Jornada Bebidas e Saúde, iBeSa- Instituto de Bebidas e Saúde, In Jornada Bebidas e Saúde, iBeSa- Instituto de Bebidas e Saúde, Porto. |
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16. |
Coimbra, Manuel A; Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Domingues, M. R; Vilanova, Manuel. 2006. "Immunomodulator Properties of Coffee Infusion Mannans", Trabalho apresentado em COST 926/927, In Molecular and physiological effects of bioactive food compounds, Viena. |
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17. |
Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Domingues, M. R; Vilanova, Manuel; Coimbra, Manuel A. 2006. "Immunomodulator activity of coffee infusions acetylated mannans", Trabalho apresentado em XV National Congress of Biochemistry, In XVth National Congress of Biochemistry, Aveiro. |
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Apresentação oral de trabalho Oral work presentation |
1. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. Enzymatically assays for structural study of coffee galactomannans,9th Carbohydrate Bioengineering Meeting,Lisboa,2011 (Poster). |
2. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. Stability upon roasting of coffee galactomannans and arabinogalactans,First International Congress on Cocoa Coffee and Tea,Novara,2011 (Poster). |
3. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. Increase on extractability of immunostimulatory mannans from coffee residue by a roasted procedure,COST 927,Aveiro,2009 (Comunicação). |
4. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. Chemical characterization of coffee residue mannans as affected by roasting,8th International Meeting of the Portuguese Carbohydrate Group,Braga,2009 (Poster). |
5. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. Naturally and Chemically Acetylation of Immunostimulatory Coffee Mannans,XXIV International Carbohydrate Symposium,Oslo,2008 (Poster). |
6. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. Structural features between mannans with and without imunostimulatory activities,IV Iberian Carbohydrate Meeting,Santiago de Compostela,2008 (Poster). |
7. |
Simões, Joana; Nunes, Fernando M; Domingues, M. R; Coimbra, Manuel A. Characterization by mass spectrometry of acetylated mannans of Aloe vera,4th Annual Meeting of the Portuguese Proteomics Network/ 1st Meeting of the Portuguese National Mass Spectrometry Network,Aveiro,2007 (Poster). |
8. |
Simões, Joana; Coimbra, Manuel A; Domingues, M. R; Nunes, Fernando M; Madureira, Pedro; Vilanova, Manuel. Estudo das mananas do café como fonte de compostos bioactivos,4ª Jornada Bebidas e Saúde, iBeSa- Instituto de Bebidas e Saúde,Porto,2007 (Comunicação). |
9. |
Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Domingues, M. R; Vilanova, Manuel; Coimbra, Manuel A. Characterization by mass spectrometry of chemically acetylated mannans obtained from coffee residue and presenting immunostimulatory
activity,7th International Meeting of the Portuguese Carbohydrate Group,Oeiras,2007 (Poster). |
10. |
Simões, Joana; Madureira, Pedro; Nunes, Fernando M; Domingues, M. R; Vilanova, Manuel; Coimbra, Manuel A. Immunomodulator activity of coffee infusions acetylated mannans,XV National Congress of Biochemistry,Aveiro,2006 (Poster). |
Indicadores de produção
(Production indicators)
Produção científica
Scientific production |
Produção técnica
Technical production |
Produção científica Scientific production |
25 |
Livros e capítulos Books and book chapters |
1 |
Capítulos de livros publicados Published book chapters |
1 |
Artigos científicos em revistas Papers in periodics |
7 |
Com arbitragem científica With scientific refereeing |
7 |
Trabalhos em eventos Papers in conference proceedings |
17 |
Com arbitragem científica With scientific refereeing |
17 |
Produção técnica Technical production |
10 |
Outros tipos de produção técnica Other technical production |
10 |
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