Catarina de Matos Ferraz Franco. Concluiu Bioquimica pelo Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa em 2011. É Bolseira de Pós-Doutoramento e Investigador Principal no Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa. Publicou 12 artigos em revistas especializadas e 27 trabalhos em actas de eventos. Possui 34 itens de produção técnica. Orientou 6 trabalhos de iniciação científica na área de Ciências Biológicas. Recebeu 3 prémios e/ou homenagens. Actualmente participa em 2 projectos de investigação, sendo que coordena 1 destes. Actua nas áreas de Ciências Naturais com ênfase em Ciências Biológicas, Engenharia e Tecnologia com ênfase em Biotecnologia Industrial e Ciências Médicas com ênfase em Biotecnologia Médica. Nas suas actividades profissionais interagiu com 76 colaboradores em co-autorias de trabalhos científicos. No seu curriculum DeGóis os termos mais frequentes na contextualização da produção científica, tecnológica e artístico-cultural são: Mass spectrometry, MALDI-TOF/TOF, Proteomics, 2-DE, Sea star, Regeneration, yeast, enzymology, Neuroregeneration e Virus-like particles.
Endereço de acesso a este CV: http://www.degois.pt/visualizador/curriculum.jsp?key=7414430016674649

Nome completo Full name	Catarina de Matos Ferraz Franco
Nome em citações bibliográficas Quoting name	Franco, Catarina F.
Categoria profissional Position	Bolseira de Pós-Doutoramento
Domínio científico de atuação Scientific domain	Ciências Naturais-Ciências Biológicas. Engenharia e Tecnologia-Biotecnologia Industrial. Ciências Médicas-Biotecnologia Médica.

Endereço profissional Professional address

Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa Av. da República, Estação Agronómica Nacional Oeiras 2780-157 Oeiras Portugal Telefone: (+351)214469452Extensão: 1452 Correio electrónico: cfranco@itqb.unl.pt Homepage: http://www.itqb.unl.pt/labs/mass-spectrometry/group-members/catarina_franco

Sexo Gender

Feminino»Female

2007-2011	Doutoramento Phd
Bioquimica (4 anos » years) . Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa, Portugal.

2000-2004	Licenciatura Licentiate degree
Bioquímica (4 anos » years) . Universidade de Évora, Portugal.

2010-2010	Curso de curta duração Short course
Visualizing Molecular & Cellular Processes with 3D Animation. Harvard Medical School Portugal, Portugal.

2009-2009	Curso de curta duração Short course
Pathway Biology. How to analyse transcriptomics, ChIP-chip/seq and proteomi. Instituto Gulbenkian da Ciência, Portugal.

2009-2009	Curso de curta duração Short course
Espectrometria de Massa. Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa, Portugal.

2008-2008	Curso de curta duração Short course
Proteomics Data Analysis. Instituto Gulbenkian da Ciência, Portugal.

2007-2007	Curso de curta duração Short course
MS bioinformatics analysis for proteomics: A tutorial for tutors. Centre Médical Universitaire, Geneva University hospital, Suiça.

2007-2007	Curso de curta duração Short course
Finnigan LTQ Biotech Operations. ThermoFisher Scientific, Reino Unido.

2006-2006	Curso de curta duração Short course
Proteomics in Life Sciences. Escola de Ciências da Saúde da Universidade do Minho, Portugal.

2006-2006	Curso de curta duração Short course
Advanced Summer Course in Cell-Materials Interactions - Regenerative Medici. Instituto de Engenharia Biomédica, Universidade do Porto, Portugal.

2006-2006	Curso de curta duração Short course
Introduction to Fluorescence Microscopy. Instituto Gulbenkian da Ciência, Portugal.

2006-2006	Curso de curta duração Short course
Cromatografia Líquida e HPLC e Espectrometria de Massa. Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa, Portugal.

2005-2005	Curso de curta duração Short course
Fundamentos de Biologia Molecular. Insttuto de Higiene e Medicina Tropical, Portugal.

Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa

Mar/2012-Actual

Investigador Principal

Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa

Jan/2012-Actual

Bolseira de Pós-Doutoramento

Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa

Abr/2007-Dez/2011

Aluna de Doutoramento

Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa

Jan/2007-Jan/2008

Apoio a Investigação em projecto

Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa

Jan/2005-Abr/2007

Lecciona aulas de mestrado/doutoramento ITQB

Universidade do Sul da Dinamarca, Odense

Out/2005-Nov/2005

Estágio de Investigação

Instituto de Biologia Experimental e Tecnológica

Jan/2005-Out/2005

Investigação Ciêntifica

Instituto de Biologia Experimental e Tecnológica

Fev/2005-Abr/2005

Bolseira de Investigação Científica

Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa

Nov/2004-Fev/2005

Bolseiro de Investigação Científica

Universidade de Évora

Set/2004-Jan/2005

Professora Assistente

Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa

Jan/2007-Actual

Disciplinas lecionadas»Taught units: Master classes on protein identification using mass spectrometry(Monitor)

Universidade de Évora

Set/2004-Jan/2005

Disciplinas lecionadas»Taught units: Bioquímica(Docente) Práticas laboratoriais de Bioquímica(Docente)

Participação como Investigador responsável Participation as responsible Researcher

2012-2012 Radial nerve cord regeneration in different starfish species - A behavioral and cellular approach-Radial nerve cord regeneration in different starfish species - A behavioral and cellular approach

Referência do projeto»Project reference: ASSEMBLE is an EU FP7 research. ASSEMBLE (EU FP7) Parceiro(s)»Partners: The Sven Lovén Centre for Marine Sciences. Financiador(es)»Funding: EU FP7.

Participação como Doutorando Participation as Phd student

2009-2011 Molecular characterization of organ regeneration in starfish - a proteomic approach toward the discovery of new regeneration factors

Referência do projeto»Project reference: PTDC/MAR/104058/2008. Financiador(es)»Funding: Fundação para a Ciência e a Tecnologia.

Compreende Understandig	Português (Bem), Inglês (Bem), Espanhol (Pouco), Francês (Pouco).
Fala Speaking	Português (Bem), Inglês (Bem), Espanhol (Pouco), Francês (Pouco).
Lê Reading	Português (Bem), Inglês (Bem), Espanhol (Pouco), Francês (Pouco).
Escreve Writing	Português (Bem), Inglês (Bem), Espanhol (Razoavelmente), Francês (Pouco).

2009	Best poster award, 1st ICAP – International Congress on Analytical Proteomics & 5th Congress of the Portuguese Proteomi.
2010	1st prize for best oral presentation, 7th European Conference on Echinoderms: Echinoderm Research 2010" in Göttingen, Germany.
2011	Best poster award, ICAP International Congress on Analytical Proteomics, Ourense, Spain.

2004- Actual

ProCura, Membro.

Capítulos de livros publicados Published book chapters

1.	Soares, Renata; Pires, Elisabete; Almeida, A. M; Santos, Romana; Gomes, Ricardo; Koci, Kamila; Franco, Catarina F; Coelho, Ana V. 2012. Tandem Mass Spectrometry of Peptides.  In Tandem Mass Spectrometry - Applications and Principles, ed. Jeevan K. Prasain, 35 - 56. ISBN: 978-953-51-0141-3. USA: InTech.

Artigos em revistas com arbitragem científica Papers in periodics with scientific refereeing

1.	Marcelino, Isabel; de Almeida, A. M; Brito, Catarina; Meyer, Damien F; Barreto, Mónica; Sheikboudou, Christian; Franco, Catarina F; Martinez, Dominique; Lefrançois, Thierry; Vachiéry, Nathalie; Carrondo, Manuel J; Coelho, Ana V; Alves, Paula M. 2012. "Proteomic analyses of Ehrlichia ruminantium highlight differential expression of MAP1-family proteins", Veterinary Microbiology 156, 3-4: 305 - 314.

2.	Coelho, Ana V; Soares, Renata; Franco, Catarina F; Pires, Elisabete; Ventosa, Miguel; Palhinhas, Rui; Koci, Kamila; Almeida, A. M. 2012. "Mass Spectrometry and Animal Science: Protein identification strategies and particularities of farm animal species", Journal of Proteomics, Sp Issue: 1 - 15.

3.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. 2011. "Exploring the proteome of an echinoderm nervous system: 2-DE of the sea star radial nerve cord and the synaptosomal membranes subproteome", Proteomics 11, 7: 1359 - 1364. Abstract We describe the first proteomic characterization of the radial nerve cord (RNC) of an echinoderm, the sea star Marthasterias glacialis. The combination of 2-DE with MS (MALDITOF/ TOF) resulted in the identification of 286 proteins in the RNC. Additionally, 158 proteins were identified in the synaptosomal membranes enriched fraction after 1-DE separation. The 2-DE RNC reference map is available via the WORLD-2DPAGE Portal (http:// www.expasy.ch/world-2dpage/) along with the associated protein identification data which are also available in the PRIDE database. The identified proteins constitute the first highthroughput evidence that seems to indicate that echinoderms nervous transmission relies primarily on chemical synapses which is similar to the synaptic activity in adult mammal’s spinal cord. Furthermore, several homologous proteins known to participate in the regeneration events of other organisms were also identified, and thus can be used as targets for future studies aiming to understand the poorly uncharacterized regeneration capability of echinoderms. This ‘‘echinoderm missing link’’ is also a contribution to unravel the mystery of deuterostomian CNS evolution. Important note: This paper was highlighted in the cover of the journal and also had a highlight in goProteomics (http://www.wiley-vch.de/goproteomics/) "picture of the month".

4.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. 2011. "Proteome characterization of sea star coelomocytes - the innate immune effector cells of echinoderms", Proteomics 1, 1: 1 - 2. ABSTRACT Sea star coelomic fluid is in contact with all internal organs, carrying signaling molecules and a large population of circulating cells, the coelomocytes. These cells, also known as echinoderm blood cells, are responsible for the innate immune responses and are also known to have an important role in the first stage of regeneration, i.e. wound closure, necessary to prevent disruption of the body fluid balance and to limit the invasion of pathogens. This work focuses on the proteome characterization of these multifunctional cells. The identification of 358 proteins was achieved using a combination of two techniques for protein separation (1D SDS PAGE followed by nanoLC and 2D SDS-PAGE) and MALDI-TOF/TOF mass spectrometry for protein identification. To our knowledge, the present report represents the first comprehensive list of sea star coelomocyte proteins, constituting an important database to validate many echinoderm predicted proteins. Evidence for new pathways in these particular echinoderm cells are also described, thus representing a valuable resource to stimulate future studies aiming to unravel the homology with vertebrate immune cells and particularly the origins of the immune system itself. .

5.	Campos, Alexandre; Carvajal-Vallejos, P. K; Villalobos, E.; Franco, Catarina F; Almeida, A. M; Coelho, Ana V; Torne, J. M; Santos, M.. 2010. "Characterisation of Zea mays L. plastidial transglutaminase: interactions with thylakoid membrane proteins", Plant Biology 12, 5: 708 - 716. ABSTRACT Chloroplast transglutaminase (chlTGase) activity is considered to play a significant role in response to a light stimulus and photo-adaptation of plants, but its precise function in the chloroplast is unclear. The characterisation, at the proteomic level, of the chlTGase interaction with thylakoid proteins and demonstration of its association with photosystem II (PSII) protein complexes was accomplished with experiments using maize thylakoid protein extracts. By means of a specific antibody designed against the C-terminal sequence of the maize TGase gene product, different chlTGase forms were immunodetected in thylakoid membrane extracts from three different stages of maize chloroplast differentiation. These bands co-localised with those of lhcb 1, 2 and 3 antenna proteins. The most significant, a 58 kDa form present in mature chloroplasts, was characterised using biochemical and proteomic approaches. Sequential fractionation of thylakoid proteins from light-induced mature chloroplasts showed that the 58 kDa form was associated with the thylakoid membrane, behaving as a soluble or peripheral membrane protein. Two-dimensional gel electrophoresis discriminated, for the first time, the 58-kDa band in two different forms, probably corresponding to the two different TGase cDNAs previously cloned. Electrophoretic separation of thylakoid proteins in native gels, followed by LC-MS mass spectrometry identification of protein complexes indicated that maize chlTGase forms part of a specific PSII protein complex, which includes LHCII, ATPase and pSbS proteins. The results are discussed in relation to the interaction between these proteins and the suggested role of the enzyme in thylakoid membrane organisation and photoprotection. Citations:1.

6.	Batista, Ana P; Franco, Catarina F; Mendes, Marta; Coelho, Ana V; Pereira, Manuela M. 2010. "Subunit composition of Rhodothermus marinus respiratory complex I ", Analytical Biochemistry 407, 1: 104 - 110. Abstract The basic structural characterization of complex I is still not trivial due to its complexity, not only in the number of its protein constituents but especially because of the different properties of the several subunits. Bacterial complex I generally contains 14 subunits: 7 hydrophilic proteins located in the peripheral arm and 7 hydrophobic proteins present in the membrane arm. It is the identification of the hydrophobic proteins that makes the characterization of complex I, and of membrane proteins in general, very difficult. In this article, we report the identification of the subunits of complex I from Rhodothermus marinus. The original approach, presented here, combined several protein and peptides separation strategies (different reversed phase materials, high-performance liquid chromatography, and gel electrophoresis) with different identification methods (electrospray ionization–tandem mass spectrometry, matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry, and Edman degradation analysis) and represents a step forward in the characterization of membrane proteins that studies are still technically highly challenging. The combination of the different methodologies allowed the identification of complex I canonical subunits and also a possible novel subunit, namely a pterin-4a-carbinolamine dehydratase (PCD). This was the first time that a PCD was suggested to be part of complex I, and its possible regulatory role is discussed. Citations:1.

7.	Lamy, Elsa; Graça, Gonçalo; Costa, Gonçalo; Franco, Catarina F; Silva, Fernando C. e; Baptist, Elvira S; Coelho, Ana V. 2010. "Changes in mouse whole saliva soluble proteome induced by tannin-enriched diet", Proteome Sciene 8, 65: 65 - 77. Abstract Background: Previous studies suggested that dietary tannin ingestion may induce changes in mouse salivary proteins in addition to the primarily studied proline-rich proteins (PRPs). The aim of the present study was to determine the protein expression changes induced by condensed tannin intake on the fraction of mouse whole salivary proteins that are unable to form insoluble tannin-protein complexes. Two-dimensional polyacrylamide gel electrophoresis protein separation was used, followed by protein identification by mass spectrometry. Results: Fifty-seven protein spots were excised from control group gels, and 21 different proteins were identified. With tannin consumption, the expression levels of one a-amylase isoform and one unidentified protein increased, whereas acidic mammalian chitinase and Muc10 decreased. Additionally, two basic spots that stained pink with Coomassie Brilliant Blue R-250 were newly observed, suggesting that some induced PRPs may remain uncomplexed or form soluble complexes with tannins. Conclusion: This proteomic analysis provides evidence that other salivary proteins, in addition to tannin-precipitating proteins, are affected by tannin ingestion. Changes in the expression levels of the acidic mammalian chitinase precursor and in one of the 14 salivary a-amylase isoforms underscores the need to further investigate their role in tannin ingestion. Citations:1.

8.	Franco, Catarina F; Mellado, Maria C. M; Alves, Paula M; Coelho, Ana V. 2010. "Monitoring virus-like particle and viral protein production by intact cell MALDI-TOF mass spectrometry", Talanta 80, 4: 1561 - 1568. Abstract A new application of intact cell MALDI-TOF MS (ICM-MS) methodology is described for monitoring the production of viral proteins and viral like particles using the baculovirus/insect cells expression system. Various MALDI matrices, cell preparation methods, cell/matrix volume ratio and MALDI target application procedures were tested in order to obtain the highest intensity and reproducibility of intact insect cell spectra. The web interface, SPECLUST (http://bioinfo.thep.lu.se/speclust.html), was used to construct dendograms based on MALDI-TOF MS data for evaluation of fingerprint changes. We demonstrate that insect cell mass spectrum fingerprints are characteristic of each viral protein/particle production. Their changes along the time for each production experiment correlate with the intracellular viral protein content determined by Western blot. This work shows that this simple, fast and low cost assay, which requires low sample volume, is a powerful analytical tool that complements the most common analytical methods used for monitoring bioprocesses and has potential application in the biotechnological industry namely, in the production of recombinant proteins. Citations:2.

9.	Santos, Romana; Costa, Gonçalo; Franco, Catarina F; Alves, Patricia; Coelho, Ana V. 2009. "First Insights into the Biochemistry of Tube Foot Adhesive from the Sea Urchin Paracentrotus lividus (Echinoidea, Echinodermata)", Mar Biotechnol 11, 11: 686 - 698. Abstract Sea urchins are common inhabitants of waveswept shores. To withstand the action of waves, they rely on highly specialized independent adhesive organs, the adoral tube feet. The latter are extremely well-designed for temporary adhesion being composed by two functional subunits: (1) an apical disc that produces an adhesive secretion to fasten the sea urchin to the substratum, as well as a deadhesive secretion to allow the animal to move and (2) a stem that bears the tensions placed on the animal by hydrodynamism. Despite their technological potential for the development of new biomimetic underwater adhesives, very little is known about the biochemical composition of sea urchin adhesives. A characterization of sea urchin adhesives is presented using footprints. The latter contain inorganic residues (45.5%), proteins (6.4%), neutral sugars (1.2%), and lipids (2.5%). Moreover, the amino acid composition of the soluble protein fraction revealed a bias toward six amino acids: glycine, alanine, valine, serine, threonine, and asparagine/aspartic acid, which comprise 56.8% of the total residues. In addition, it also presents higher levels of proline (6.8%) and half-cystine (2.6%) than average eukaryotic proteins. Footprint insolubility was partially overcome using strong denaturing and reducing buffers, enabling the visualization of 13 proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The conjugation of mass spectrometry with homology–database search allowed the identification of six proteins: alpha and beta tubulin, actin, and histones H2B, H3, H2A, and H4, whose location and function in the adhesive are discussed but require further investigation. For the remaining unidentified proteins, five de novo-generated peptide sequences were found that were not present in the available protein databases, suggesting that they might be novel or modified proteins. Citations:4.

10.	Mellado, Maria C. M; Franco, Catarina F; Coelho, Ana V; Alves, Paula M; Simplício, Ana L. 2008. "Sodium dodecyl sulfate-capillary gel electrophoresis analysis of rotavirus-like particles", Journal of Chromatography A 1192, 1: 166 - 172. Abstract This work describes the application of a sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE) method for the analysis of triple 2/6/7 and double-layered 2/6 rotavirus-like particles (RLPs), candidate vaccines against rotavirus infection. SDS-CGE analysis of RLPs resulted in peaks that could be attributed to the viral proteins (VP2, VP6 and VP7) according to their apparent molecular mass (MWapp). Samples containing the glycoprotein VP7 were analysed after deglycosylation with PNGase F. Upon deglycosylation, VP7 MWapp decreased 4–7 kDa consistent with a degree of glycosylation of approximately 12–21%. VP2 was eventually detected in the form of more than one related proteins, despite the small areas due to the relative low mass proportion of this protein in the particle (16%). The effect of analytical parameters such as capillary temperature on method performance was evaluated. MWapp values estimated by SDS-CGE were compared with values obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The method described in this work proved to be fast, consistent and reproducible, representing a feasible alternative to the laborious conventional electrophoresis for the characterization of RLPs. Citations: 7.

11.	Ferreira, Rui; Alves-Pereira, Isabel; Magriço, Susana; Franco, Catarina. 2007. "Comportamento celular e resposta antioxidante diferenciados de Saccharomyces cerevisiae e de Saccharomyces chevalieri ao metavanadato de amónio", Rev. de Ciências Agrárias 30, 1: 455 - 464. ABSTRACT The fermentation of wine is a complex microbiological process which requires yeast adaptation to stress conditions. In the cellular environment of aerobic organisms naturally reactive oxygen species (ROS) occurs as by-products of mitochondrial respiration. The higher reactivity of these chemical species could cause molecular damages that in several cases induce cellular death. In common physiological conditions or as response to oxidative stress, the cell can generate adapted responses which involve antioxidants mechanisms as glutathione reductase (GR; EC 1.6.4.2) and catalase T (CAT T; EC 1.11.1.6) and A (CAT A; EC 1.11.1.6) enzymes.Vanadium, a heavy metal present in several pesticides could generate ROS changing the intracellular redox state and cause deleterious effects in yeasts exposed to higher levels of this element. The main objective of this work was to compare the effects of ammonium metavanadate (NH4VO3), a pentavalent salt of vanadium on cellular viability and GR, CAT T and CAT A activities of wine yeast Saccharomyces cerevisiae UE-ME3 and Saccharomyces chevalieri UE-ME1. The results obtained show that S. chevalieri UE-ME1 has lower tolerance to NH4VO3 than S. cerevisiae UE-ME3, since S. chevalieri cultures do not survive to concentration values of ammonium metavanadate higher than 7,5 mM, whereas S. cerevisiae cells are still viable in the presence of 75 mM. S. chevalieri has an enzymatic activity lower than S. cerevisiae, although for both yeast species NH4VO3 could behave as oxidative stress inductor, causing a significant decrease of GR activity (P<0,01) and a significant increase of CAT A activity (P<0,01). The results show also an increase of CAT T activity in both yeast species, which can be interpreted as a protective response to oxidative stress. Differences on response to amonium metavanadate by both species of Saccharomyces could be partially justified by more efficient antioxidant systems in S. cerevisiae UE-ME3. .

12.	Chen, Zhenjia; Franco, Catarina F; Baptista, Ricardo P; Cabral, Joaquim M. S; Coelho, Ana V; J, Carlos; Melo, Eduardo P. 2007. "Purification and identification of cutinases from Colletotrichum kahawae and Colletotrichum gloeosporioides", Applied Microbiology and Biotechnology 73, 6: 1306 - 1313. Abstract Colletotrichum kahawae is the causal agent of the coffee berry disease, infecting leaves and coffee berries at any stage of their development. Colletotrichum gloeosporioides is the causal agent of brown blight, infecting ripe berries only. Both fungi secrete the same pattern of carboxylesterases to the fermentation broth when cutin is used as carbon source. By using two different strategies composed of two precipitation steps (ammonium sulphate and acetic acid precipitation) and two chromatographic steps, two proteins displaying carboxylesterase activity were purified to electrophoretic homogeneity. One, with a molecular weight (MW) of 21 kDa, has a blocked N terminus and was identified as cutinase by peptide mass fingerprint and mass spectrometry/ mass spectrometry data acquired after peptide derivatization with 4-sulphophenyl isothiocyanate. The second, with a MW of 40 kDa, displays significant carboxylesterase activity on tributyrin but low activity on pnitrophenyl butyrate. N-terminal sequencing for this protein does not reveal any homology to other carboxylesterases. These two enzymes, which were secreted by both fungi, appear homologous. Citations: 17.

Trabalhos completos/resumidos em eventos com arbitragem científica Papers in conference proceedings with scientific refereeing

1.	Franco, Catarina F; Soares, Renata; Santos, Romana; Coelho, Ana V. 2011. "Differential phosphoproteome of the regenerating radial nerve cord of the sea star M. glacialis", Trabalho apresentado em 2nd International Congress on Analytical Proteomics, In Book of abstracts of the 2nd International Congress on Analytical Proteomics, Ourence.

2.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. 2011. "Proteomics reveals the protein degradation pathways present in the radial nerve cord of the sea star wound healing events and early stages of arm regeneration", Trabalho apresentado em Cell Biology of the Neuron Polarity Plasticity and Regeneration, In Book of abstracts of the EMBO conference Cell Biology of the Neuron Polarity Plasticity and Regeneration, Heraklion .

3.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. 2011. "Proteomics reveals the “Phoenix myth” in neuroregeneration events: The degradation pathways within the regenerating radial nerve cord of a sea star", Trabalho apresentado em 2nd International Congress on Analytical Proteomics, In Book of abstracts of the 2nd International Congress on Analytical Proteomics, Ourense.

4.	Santos, Romana; Barreto, Angela; Franco, Catarina F; Coelho, Ana V. 2010. "Using proteomics to find new bioadhesives", Trabalho apresentado em 4th EUPA Metting/6th ProCura Metting, In Book of abstracts of the 4th EUPA Metting, Estoril.

5.	Almeida, A. M; Carvalho, M.; Franco, Catarina F; R., Francisco; T, Kilminster; Scanlon, T; Greef, J.; Milton, J.; Oldham, C.; L.A., Cardoso; Coelho, Ana V. 2010. "Weight loss and the hepatic proteome in african fat tailed, European and intermediate domestic sheep breeds", Trabalho apresentado em 4th EUPA Metting/6th ProCura Metting, In Book of abstract of the 4th EUPA Metting, Estoril.

6.	Franco, Catarina F; Soares, Renata; Santos, Romana; Coelho, Ana V. 2010. "Phosphoproteome of the radial nerve cord of the sea star M. glacialis –Preliminary results", Trabalho apresentado em 4th EUPA Metting/6th ProCura Metting, In Book of Abstract of the 4th EUPA Metting, Estoril.

7.	Gomes, Ricardo; Franco, Catarina F; S., Planchon; J, Renaut; Costa, Gonçalo; Outeiro, Tiago; Cordeiro, Carlos; Coelho, Ana V. 2010. "rotein misfolding and aggregation in vivo: Identification of the cellular response mechanisms by 2DE-DIGE", Trabalho apresentado em 4th EUPA Metting/6th ProCura Metting,, In Book of abstracts of the 4th EUPA Metting, Estoril.

8.	Almeida, A. M; I., Marcelino; Franco, Catarina F; Alves, Paula M; M.J.T, Carrondo; Coelho, Ana V. 2010. "Ehrlichia ruminantium elementary body proteome: establishment of a 2DE reference map", Trabalho apresentado em 4th EUPA Metting/6th ProCura Metting, In Book of abstracts of the 4th EUPA Metting, Estoril.

9.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. 2010. "Sea star coelomic fluid proteome characterization part I: Cell free coelomic fluid proteome, the sea stars cell/Tissue talk show", Trabalho apresentado em 4th EUPA Metting/6th ProCura Metting, In Book of abstracts of the 4th EUPA Metting, Estoril.

10.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. 2010. "Sea star coelomic fluid proteome characterization part II: Coelomocytes, the super-cells of echinoderms", Trabalho apresentado em 4th EUPA Metting/6th ProCura Metting, In Book of abstracts of the 4th EUPA Metting, Estoril.

11.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. 2010. "Exploring the proteome of an echinoderm nervous system: 2-DE of the sea star radial nerve cord and the synaptosomal membranes subproteome", Trabalho apresentado em 7th European Conference on Echinoderms: Echinoderm Research 2010" , In Book of abstract of the 7th European Conference on Echinoderms: Echinoderm Research, Göttingen. Awarded with BEST ORAL COMMUNICATION.

12.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. 2010. "In-depth/High resolution 2D-DIGE to pin-point protein candidates actively involved in the regeneration events of star radial nerve cord", Trabalho apresentado em 4th EUPA Metting/6th ProCura Metting, In Book of abstracts of the 4th EUPA Metting, Estoril.

13.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. 2009. "Starfish marthasterias glacialis radial nerve cord proteome", Trabalho apresentado em 3rd EuPA Congress, In 3rd EuPA Congress Proceedinds, Stockholm.

14.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. 2009. "Starfish Marthasterias glacialis radial nerve cord proteome", Trabalho apresentado em 1st ICAP-International Congress on Analytical Proteomics & 5th Congress of the Portuguese Proteomics Network, In Book of abstracts of 1st ICAP & 5th Congress of the Portuguese Proteomics Network, Costa da Caparica.

15.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. 2009. "Advances in neuropeptidomics of the Marthasterias glacialis", Trabalho apresentado em st ICAP – International Congress on Analytical Proteomics & 5th Congress of the Portuguese Proteomics Network, In Book of abstracts of st ICAP – International Congress on Analytical Proteomics & 5th Congress of the Portuguese Proteomics Network, Costa da Caparica. Awarded with BEST POSTER.

16.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. 2008. "Proteomics based approach to understanding tissue regeneration – Seastar as a model organism,The Molecular and cellular basis of Regeneration and tissue repair", Trabalho apresentado em EMBO Conference Series, In Book of abstracts of Palma de Maiorca, Palma de Maiorca.

17.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. 2007. "Proteomics based approach to understanding tissue regeneration – Seastar as a model organism", Trabalho apresentado em ProCura 2007 Metting, In Book of abstracts of ProCura 2007 Metting, Aveiro.

18.	Franco, Catarina F; Coelho, Ana V. 2007. "On the Quest of Protein identification and Proteome knowledge", Trabalho apresentado em ProCura 2007 Metting, In Book of abstract of ProCura 2007 Metting, Aveiro.

19.	Alves-Pereira, Isabel; Magriço, Susana; Ferreira, Rui; Franco, Catarina F. 2006. "Comparative effects of NH4VO3 on detoxification enzymes and redox state of wine Saccharomyces", Trabalho apresentado em 9th International Symposium on Metal Ions in Biology an Medicine, In Book of Abstracts of 9th International Symposium on Metal Ions in Biology an Medicine, Lisboa.

20.	Franco, Catarina F; A.C., Dourado; Alves, Paula; Coelho, Ana V; J.F, Marques; P, Fareleira; T.M.T, Crespo. 2006. "Diversity of medicago-nodulating rhizobizal strains from southern portugal-identification and characterization using mass spectrometry approach", Trabalho apresentado em From Genome to Proteome: Back to the Future, In Book of Abstracts of From Genome to Proteome: Back to the Future, Siena.

21.	Franco, Catarina F; Sousa, Marcos; Silva, Carina; Alves, Paula M; Coelho, Ana V. 2006. "Monitorizing structural integrity of rotavirus like particles using mass spectrometry", Trabalho apresentado em Congresso Nacional de Bioquímica, In Livro de resumos do Congresso Nacional de Bioquímica, Aveiro.

22.	Franco, Catarina F; Alves, Paula; Coelho, Ana V; J.F, Marques; P, Fareleira; T.M.T, Crespo. 2005. "Diversity of Medicago-nodulating rhizobial strains from Southern Portugal.", Trabalho apresentado em Micro-Biotec-2005 Congress, In Book of Abstracts Micro-Biotec-2005, Braga.

23.	Franco, Catarina F; Graça, Gonçalo; Costa, Gonçalo; Coelho, Ana V. 2005. "De novo sequencing of saliva proteins tryptic digests by PSD MALDI-MS using SPITC as a N-terminal sulfonation reagent.", Trabalho apresentado em Congresso Sociedade Proteómica Espanhola, In Book of Abstracts of the Congresso Sociedade Proteómica Espanhola, Córdoba.

24.	Franco, Catarina F; Alves, Paula; P, Fareleira; T.M.T, Crespo; Coelho, Ana V. 2005. "Identification and characterization of soil bacteria using Mass Spectrometry approach-Fingerprinting Sinorhizobium cell surface proteins by MALDI-TOF Mass Spectrometry", Trabalho apresentado em 3º Encontro Nacional da Procura, In Livro de Resumos do 3º Encontro Nacional da Procura, Cascais.

25.	Franco, Catarina; Alves-Pereira, Isabel; Ferreira, Rui. 2004. "Efeitos do Metavanadato de Amónio nos enzimas Glutationo S-transferase e g-Glutamil transpeptidades da levedura Saccharomyces chevalieri UE-ME1", Trabalho apresentado em 8ª Conferência Nacional do Ambiente, In livro de actas da 8ª Conferência Nacional do Ambiente, Belém.

26.	Franco, Catarina; Alves-Pereira, Isabel; Ferreira, Rui. 2004. "Effects on cellular viability and on the yeast Saccharomyces chevalieri NADPH (P450)reductase", Trabalho apresentado em XIV Congresso Nacional de Bioquímica, In Livro de Abstracts do XIV Congresso Nacional de Bioquímica, Vilamora.

27.	Franco, Catarina; Alves-Pereira, Isabel; Ferreira, Rui. 2004. "Efeitos do metavanadato de Amónio nos enzimas glutaiono S-transferase e g-glutamil transpeptidase da levedura Saccharomyces chevalieri UE-ME1", Trabalho apresentado em Conferência Nacional do Ambiente, Centro Cultural de Belém,, In Livro de Resumos da Conferência Nacional do Ambiente, Belém.

Apresentação oral de trabalho Oral work presentation

1.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. Proteomics reveals the “Phoenix myth” in neuroregeneration events: The degradation pathways within the regenerating radial nerve cord of a sea star ,2nd International Congress on Analytical Proteomics 18th-20th July 2011,Ourense,2011 (Seminário).

2.

Franco, Catarina F; Santos, Romana; Coelho, Ana V. Proteomics reveals the protein degradation pathways present in the radial nerve cord of the sea star wound healing events and early stages of arm regeneration,Cell Biology of the Neuron Polarity Plasticity and Regeneration,Heraklion Greece 7 10 May 2011,2011 (Poster).

3.	Franco, Catarina F; Soares, Renata; Pires, Elisabete; Santos, Romana; Coelho, Ana V. Differential phosphoproteome of the regenerating radial nerve cord of the sea star M. glacialis,2nd International Congress on Analytical Proteomics,Ourence,2011 (Poster).

4.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. Proteomics in regeneration processes,Hands-On Course on Protein Identification by Mass Spectrometry. ITQB-UNL. 2-4th November 2011, Oeiras, Portugal,2011 (Seminário).

5.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. In-depth/High resolution 2D-DIGE to pin-point protein candidates actively involved in the regeneration events of star radial nerve cord,4th EUPA Metting/6th ProCura Metting, Estoril, Portugal.,Estoril, Portugal.,2010 (Comunicação).

6.

Franco, Catarina F; Santos, Romana; Coelho, Ana V. Exploring the proteome of an echinoderm nervous system: 2-DE of the sea star radial nerve cord and the synaptosomal membranes subproteome,7th European Conference on Echinoderms: Echinoderm Research 2010" in Göttingen, Germany ,Göttingen, Germany ,2010 (Comunicação). Awarded 1st prize for best oral presentation.

7.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. Sea star coelomic fluid proteome characterization part II: Coelomocytes, the super-cells of echinoderms,4th EUPA Metting/6th ProCura Metting,Estoril, Portugal,2010 (Poster).

8.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. Sea star coelomic fluid proteome characterization part I: Cell free coelomic fluid proteome, the sea stars cell/Tissue talk show,4th EUPA Metting/6th ProCura Metting,Estoril, Portugal,2010 (Poster).

9.	Almeida, A. M; I., Marcelino; Franco, Catarina F; M.J.T, Carrondo; Alves, Paula M; Coelho, Ana V. Ehrlichia ruminantium elementary body proteome: establishment of a 2DE reference map,4th EUPA Metting/6th ProCura Metting,Estoril, Portugal,2010 (Poster).

10.

Gomes, Ricardo; Franco, Catarina F; S., Planchon; J, Renaut; Costa, Gonçalo; Outeiro, Tiago; Cordeiro, Carlos; Coelho, Ana V. Protein misfolding and aggregation in vivo: Identification of the cellular response mechanisms by 2DE-DIGE,4th EUPA Metting/6th ProCura Metting,Estoril, Portugal,2010 (Poster).

11.	Franco, Catarina F; Soares, Renata; Santos, Romana; Coelho, Ana V. Phosphoproteome of the radial nerve cord of the sea star M. glacialis –Preliminary results,4th EUPA Metting/6th ProCura Metting,Estoril, Portugal,2010 (Poster).

12.

Almeida, A. M; Carvalho, M.; Franco, Catarina F; R., Francisco; T, Kilminster; Scanlon, T; Greef, J.; Milton, J.; Oldham, C.; L.A., Cardoso; Coelho, Ana V. Weight loss and the hepatic proteome in african fat tailed, European and intermediate domestic sheep breeds,4th EUPA Metting/6th ProCura Metting,Estoril, Portugal,2010 (Comunicação).

13.	Santos, Romana; Barreto, Angela; Franco, Catarina F; Coelho, Ana V. Using proteomics to find new bioadhesives,4th EUPA Metting/6th ProCura Metting,Estoril, Portugal,2010 (Poster).

14.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. Advances in neuropeptidomics of the starfish Marthasterias glacialis,st ICAP – International Congress on Analytical Proteomics & 5th Congress of the Portuguese Proteomics Network,Costa da Caparica,2010 (Poster). BEST POSTER AWARD.

15.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. Starfish Marthasterias glacialis radial nerve cord proteome,1st ICAP – International Congress on Analytical Proteomics & 5th Congress of the Portuguese Proteomics Network, Costa da Caparica, Portugal,Costa da Caparica, Portugal,2009 (Comunicação).

16.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. Proteomics based approach to understand tissue regeneration-Starfish as a model organism,PhD Seminar,Oeiras,2009 (Comunicação).

17.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. Starfish Marthasterias glacialis radial nerve cord proteome,3rd EuPA Metting,Estocolmo,2009 (Poster).

18.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. Proteomics based approach to understanding tissue regeneration – Seastar as a model organism,The Molecular and cellular basis of Regeneration and tissue repair. EMBO Conference Series,Palma de Maiorca,2008 (Poster).

19.	Franco, Catarina F; Coelho, Ana V. On the Quest of Protein identification and Proteome knowledge,ProCura 2007 Metting,Aveiro,2007 (Comunicação).

20.	Franco, Catarina F; Santos, Romana; Coelho, Ana V. Proteomics based approach to understanding tissue regeneration – Seastar as a model organism,ProCura 2007 Metting,Aveiro,2007 (Poster).

21.	Franco, Catarina F; Sousa, Marcos; Silva, Carina; Alves, Paula M; Coelho, Ana V. Monitorizing structural integrity of rotavirus like particles using mass spectrometry,Congresso Nacional de Bioquímica,Aveiro,2006 (Poster).

22.

Franco, Catarina F; A.C., Dourado; P.I., Alves; Coelho, Ana V; J.F, Marques; P, Fareleira; T.M.T, Crespo. Diversity of medicago-nodulating rhizobizal strains from southern portugal-identification and characterization using mass spectrometry approach,From Genome to Proteome: Back to the Future,Siena, Itália,2006 (Poster).

23.	Alves-Pereira, Isabel; Magriço, Susana; Ferreira, Rui; Franco, Catarina. Comparative effects of NH4VO3 on detoxification enzymes and redox state of wine Saccharomyces,9th International Symposium on Metal Ions in Biology an Medicine,Lisboa,2006 (Poster).

24.

Franco, Catarina F; P.I., Alves; P, Fareleira; T.M.T, Crespo; Coelho, Ana V. Identification and characterization of soil bacteria using Mass Spectrometry approach-Fingerprinting Sinorhizobium cell surface proteins by MALDI-TOF Mass Spectrometry,3º Encontro Nacional da Procura, Cascais,Cascais,2005 (Comunicação).

25.	Franco, Catarina F; Graça, Gonçalo; Costa, Gonçalo; Coelho, Ana V. De novo sequencing of saliva proteins tryptic digests by PSD MALDI-MS using SPITC as a N-terminal sulfonation reagent,Congresso Sociedade Proteómica Espanhola, Universidade de Córdoba,Córdoba,2005 (Poster).

26.	Franco, Catarina F; Alves, Paula; Coelho, Ana V; J.F, Marques; P, Fareleira; T.M.T, Crespo. Diversity of Medicago-nodulating rhizobial strains from Southern Portugal. ,Micro-Biotec-2005 Congress,Braga,2005 (Poster).

27.	Franco, Catarina F; Alves-Pereira, Isabel; Ferreira, Rui. Efeitos do metavanadato de Amónio nos enzimas glutaiono S-transferase e g-glutamil transpeptidase da levedura Saccharomyces chevalieri UE-ME1,Conferência Nacional do Ambiente, Centro Cultural de Belém,Belém,2004 (Poster).

28.	Franco, Catarina; Alves-Pereira, Isabel; Ferreira, Rui. Effects on cellular viability and on the yeast Saccharomyces chevalieri NADPH (P450)reductase,XIV Congresso Nacional de Bioquímica,Vilamora,2004 (Poster).

Curso de curta duração lecionado Taught short course

1.	Franco, Catarina F. Hands-On Course on Protein Identification by Mass Spectrometry, 2011 (Especialização), promovido por ITQB-UNL. Duração: 1 semanas. Local: ITQB-UNL, Cidade: Oeiras, Tipo de participação: Organizador.

2.	Franco, Catarina F; Coelho, Ana V. Curso de pós-graduação “Proteomics in Life Sciences", 2006 (Especialização), promovido por Escola de Ciências da Saúde da Universidade do Minho. Duração: 5 horas. Local: Universidade do Minho, Cidade: Braga, Tipo de participação: Docente.

3.	Franco, Catarina F; Coelho, Ana V. Identification of Proteins Using Mass Spectrometry Data, 2005 (Especialização), promovido por ITQB com colaboração SPROT e ThermoUnicam. Duração: 1 dias. Local: ITQB, Cidade: Oeiras, Tipo de participação: Docente.

Organização de evento Event organization

1.	Franco, Catarina F; Coelho, Ana V; Santos, Romana. First RNEM course on Protein Identification by Mass spectrometry,2010 (Oficina / Organização).

2.	Franco, Catarina F; Coelho, Ana V. Whorkshop no âmbito da Rede Nacional de Espectrometria de Massa,2005 (Encontro / Organização).

3.	Franco, Catarina F; Pires, Elisabete; Coelho, Ana V. Identification of Proteins Using Mass Spectrometry Data,2005 (Oficina / Outra).

Obra de artes visuais Visual art production

1.	Franco, Catarina F. 3D Animation of sea star regeneration process,2010 (Vídeo).

Orientações Orientations

Orientações Orientations

Dissertação de Mestrado Master degree dissertation

Em curso Ongoing

1.	Joana Martins, Determinação das proteínas diferencialmente expressas nos coelomócitos da estrela do mar Marthasterias glacialis durante 3 fases distintas da regeneração , 2011. (Orientador).

Iniciação científica Scientific initiation

Concluídas Completed

1.	Angela Barreto, Introduction to two-dimensional electrophoresis, 2010. Iniciação científica (Proteómica) - Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa (Orientador).

2.	Ricardo Anjos, Supervised training of new MALDI-TOF/TOF users, 2008. Iniciação científica (Espectrometria de Massa) - Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa (Orientador).

3.	André Almeida, Supervised training of new MALDI-TOF/TOF users, 2008. Iniciação científica (Espectrometria de Massa) - Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa (Orientador).

4.	Peter Boross, Supervised training of new MALDI-TOF/TOF users, 2008. Iniciação científica (Espectrometria de Massa) - Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa (Orientador).

5.	Mariana Carvalho, Supervised training of new MALDI-TOF/TOF users, 2008. Iniciação científica (Espectrometria de Massa) - Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa (Orientador).

6.	New Mass Spectrometry users, Procedures for in-gel/gel free digestion of proteins/Peptide purification and sample preparation for mass spectrometry, 2007. Iniciação científica (Espectrometria de Massa) - Instituto de Tecnologia Química e Biológica - Universidade Nova de Lisboa (Orientador).

Produção científica Scientific production

Produção técnica Technical production

Produção artística/cultural Artistical/cultural production

Dados complementares data

Total

Produção científica Scientific production

40

Livros e capítulos Books and book chapters

1

Capítulos de livros publicados Published book chapters

1

Artigos científicos em revistas Papers in periodics

12

Com arbitragem científica With scientific refereeing

12

Trabalhos em eventos Papers in conference proceedings

27

Com arbitragem científica With scientific refereeing

27

Total

Produção técnica Technical production

34

Outros tipos de produção técnica Other technical production

34

Total

Produção artística/cultural Artistical/cultural production

1

Total

Dados complementares (Additional data)

7

Orientações Orientations

7

	Maria Elvira Lourido de Sales Baptista
	Miguel André Lourenço da Luz Ventosa
	Romana Lopes Almeida dos Santos

Romana Lopes Almeida dos Santos