Ana Bárbara Tavares dos Santos, doutorada, iniciou recentemente o seu primeiro Pós-Doutoramento no Laboratório Cilia Regulation and Disease, sob a orientação da Doutora Susana Lopes. Este laboratório está associado ao CEDOC e ao IGC e estuda a regulação do tamanho dos cílios e a sua relação com ciliopatias tais como o Situs Inversus, Doença Poliquística Renal e o Síndrome de Joubert, usando para tal o peixe zebra (Danio rerio) como modelo. Durante o doutoramento, realizado simultaneamente no IGC e na FCT/UNL, Ana Bárbara Tavares dos Santos isolou e caracterizou as correntes aniónicas presentes nos protoplastos de grãos de pollen de duas espécies, Arabidopsis thaliana e Lilium longiflorum. Este projecto de investigação permitiu a publicação de 2 artigos em revistas especializadas e a participação em 6 congressos científicos internacionais com 2 comunicações orais e 4 posters. Ana Bárbara Tavares dos Santos actua na área de Ciências Biológicas e mais recentemente na área de Ciências da Saúde e ao longo das suas actividades profissionais interagiu com 9 colaboradores em co-autorias de trabalhos científicos.
Endereço de acesso a este CV: http://www.degois.pt/visualizador/curriculum.jsp?key=9897892369797469

Nome completo Full name	Ana Bárbara Tavares dos Santos
Nome em citações bibliográficas Quoting name	Tavares, Bárbara
Domínio científico de atuação Scientific domain	Ciências Naturais-Ciências Biológicas. Ciências Médicas-Ciências da Saúde.

Endereço profissional Professional address

Fundação Calouste Gulbenkian Instituto Gulbenkian de Ciência R da Quinta Grande, 6 Oeiras 2781-156 Oeiras Portugal Telefone: (+351)21440790 Correio electrónico: absantos@gmail.com

Sexo Gender

Feminino»Female

2006-2011	Doutoramento Phd
Biologia - Biologia do Desenvolvimento. Universidade de Lisboa, Portugal.

2000-2005	Licenciatura Licentiate degree
Biologia Celular e Biotecnologia (4 anos » years) . Universidade de Lisboa, Portugal.

2005-2006	Especialização/ Pós-Graduação Specialization/ Postgraduation
Curso de Especialisação em Biologia. Universidade de Lisboa, Portugal.

Centro de Estudo de Doenças Cronicas

Jun/2011-Actual

Investigador Auxiliar

Fundação Calouste Gulbenkian

Out/2006-Abr/2011

Outra Situação

Set/2005-Set/2006

Estagiário Investigador

Universidade Nova de Lisboa

Out/2006-Abr/2011

Outra Situação

Set/2005-Set/2006

Estagiário Investigador

Centro de Estudo de Doenças Cronicas

Jan/2012-Actual
Linhas de investigação»Research fields: Investigação do papel do Calcio na regulação do tamanho ciliar

Fundação Calouste Gulbenkian

Out/2006-Abr/2011
Linhas de investigação»Research fields: Molecular identification of the transporter/s responsible for the anionic currents found in the plasma membrane of pollen grain protoplasts from Arabidopsis thaliana and Lilium longiflorum

Universidade Nova de Lisboa

Out/2006-Dez/2006
Linhas de investigação»Research fields: Caracterização electrofisiológica de transportadores membranares de tubo polínico de Arabidopsis thaliana e de Lilium longiflorum

Universidade Nova de Lisboa

Set/2006-Set/2006

Estágios realizados»Traineeships:    - Caracterização electrofisiológica de dois possíveis transportadores aniónicos de Arabidopsis thaliana, Clc-c e Clc-d, transientemente transcritos em células HEK293

Fundação Calouste Gulbenkian

Set/2005-Set/2006

Estágios realizados»Traineeships:    - Sub-clonagem de dois possíveis transportadores aniónicos de Arabidopsis thaliana, Clc-c e Clc-d, em vectores de tradução em células de mamífero, para futura transfecção transiente em células HEK293

Participação como Investigador Participation as Researcher

2013- Análise quantitativa da expressão assimétrica de genes resultantes de fluxos ciliares existentes no organizador da lateralidade embrionário.-Quantitative analysis of asymmetric gene expression in the left-right organizer in response to flow forces: what comes downstream of cilia driven flow?

Referência do projeto»Project reference: FCT-ANR/BEX-BID/0153/2012.

2010-2013 A investigação de uma nova via de sinalização entre DeltaD e ciliogénese e o seu impacto no desenvolvimento da identidade esquerda-direita-Investigation of a new signalling pathway linking deltaD to ciliogenesis and its impact on left-right development

Referência do projeto»Project reference: PTDC/SAU-OBD/103981/2008. Parceiro(s)»Partners: Universidade de Lisboa. Financiador(es)»Funding: Fundação para a Ciência e a Tecnologia.

Participação como Doutorando Participation as Phd student

2007-2011 Caracterização electrofisiológica de transportadores membranares de tubo polínico de Arabidopsis thaliana-Electrophysiological characterization of membrane transporters from Arabidopsis thaliana pollen tube

Referência do projeto»Project reference: PTDC/QUI/64359/2006. Parceiro(s)»Partners: Fundação Calouste Gulbenkian. Financiador(es)»Funding: Fundação para a Ciência e a Tecnologia.

Compreende Understandig	Inglês (Bem), Português (Bem).
Fala Speaking	Inglês (Bem), Português (Bem).
Lê Reading	Inglês (Bem), Português (Bem).
Escreve Writing	Inglês (Bem), Português (Bem).

Jan/2012 - Actual	Sociedade Portuguesa de Biologia de Desenvolvimento, Membro. Organizing the Development in Action Meetings.
2009- Actual	Sociedade Portuguesa de Biofisica, Membro.

Artigos em revistas com arbitragem científica Papers in periodics with scientific refereeing

1.	Tavares, Bárbara. 2013. "The Importance of Zebrafish in Biomedical Research", Acta Med Port 26, 5: 583 - 592.

2.	Tavares, Bárbara; Domingos, Patrícia; Dias, Pedro; Feijó, José; Bicho, Ana. 2011. "The essential role of anionic transport in plant cells: the pollen tube as a case study", Journal of Experimental Botany 62, 7: 2273 - 2298. Plasma membrane anion transporters play fundamental roles in plant cell biology, especially in stomatal closure and nutrition. Notwithstanding, a lot is still unknown about the specific function of these transporters, their specific localization, or molecular nature. Here the fundamental roles of anionic transport in plant cells are reviewed. Special attention will be paid to them in the control of pollen tube growth. Pollen tubes are extreme examples of cellular polarity as they grow exclusively in their apical extremity. Their unique cell biology has been extensively exploited for fundamental understanding of cellular growth and morphogenesis. Non-invasive methods have demonstrated that tube growth is governed by different ion fluxes, with different properties and distribution. Not much is known about the nature of the membrane transporters responsible for anionic transport and their regulation in the pollen tube. Recent data indicate the importance of chloride (Cl–) transfer across the plasma membrane for pollen germination and pollen tube growth. A general overview is presented of the well-known accumulated data in terms of biophysical and functional characterization, transcriptomics, and genomic description of pollen ionic transport, and the various controversies around the role of anionic fluxes during pollen tube germination, growth, and development. It is concluded that, like all other plant cells so far analysed, pollen tubes depend on anion fluxes for a number of fundamental homeostatic properties.

3.	Tavares, Bárbara; Dias, Pedro N; Domingos, Patrícia; Moura, Teresa F; Feijó, José A; Bicho, Ana. 2011. "Calcium-regulated anion channels in the plasma membrane of Lilium longiflorum pollen protoplasts", New Phytologist, 0000: 00000 - 0000. Currents through anion channels in the plasma membrane of Lilium longi¿orum pollen grain protoplasts were studied under conditions of symmetrical anionic concentrations by means of patch-clamp whole-cell con¿guration. With Cl- -based intra- and extracellular solutions, three outward-rectifying anion conductances, ICl1, ICl2 and ICl3, were identi¿ed. These three activities were discriminated by differential rundown behaviour and sensitivity to 5-nitro-2-(phenylpropylamino)-benzoate (NPPB), which could not be attributed to one or more channel types. All shared strong outward recti¿cation, activated instantaneously and displayed a slow time-dependent activation for positive potentials. All showed modulation by intracellular calcium ([Ca2+]in), increasing intensity from 6.04 nM up to 0.5 mM (ICl1), or reaching a maximum value with 8.50 lM(ICl2 and ICl3). After rundown, the anionic currents measured using NO3- -based solutions were indistinguishable, indicating that the permeabilities of the channels for Cl- and NO3- are similar. Additionally, unitary anionic currents were measured from out-side-out excised patches, con¿rming the presence of individual anionic channels. This study shows for the ¿rst time the presence of a large anionic conductance across the membrane of pollen protoplasts, resulting from the presence of Ca2+-regulated channels. A similar conductance was also found in germinated pollen. We hypothesize that these putative channels may be responsible for the large anionic ¿uxes previously detected by means of self-referencing vibrating probes.

Trabalhos completos/resumidos em eventos com arbitragem científica Papers in conference proceedings with scientific refereeing

1.	Tavares, Bárbara; Vaz, Andreia; Pintado, Petra; Lopes, Susana S. 2012. "Investigation of a New Signalling Pathway Linking DeltaD to Ciliogenesis: The Role of Rabconnectin3", Trabalho apresentado em First International Cilia in Development and Disease Scientific Conference (2012), In Proceedings of the First International Cilia in Development and Disease Scientific Conference (2012), London.

Trabalhos completos/resumidos em eventos sem arbitragem científica Papers in conference proceedings without scientific refereeing

1.	Tavares, Bárbara; Marques da S. J; Bernardes da S. A; Arrabaça, M. C. 2004. "Water stress resistance in three golf turf grasses with different photosynthetic metabolism", Trabalho apresentado em VII Simposium Luso-Espanhol de Relações Hídricas Em Plantas, In Actas do VII Simposium Luso-Espanhol de Relações Hídricas Em Plantas, Faro.

Apresentação oral de trabalho Oral work presentation

1.	Tavares, Bárbara; Vaz, Andreia; Pintado, Petra; Lopes, Susana S. The role of Rabconnectin3 in cilia length regulation,2nd Meeting of the Portuguese Society of Developmental Biology,Lisbon,2013 (Poster).

2.	Tavares, Bárbara; Vaz, Andreia; Pintado, Petra; Lopes, Susana S. Investigation of a New Signalling Pathway Linking DeltaDto Ciliogenesis: The Role of Rabconnectin3,CILIA2012 Conference - "CILIA IN DEVELOPMENT AND DISEASE",London,2012 (Poster).

3.	Tavares, Bárbara; Vaz, Andreia; Pintado, Petra; Lopes, Susana S. The Role of pH in Cilia Length Regulation,IX Meeting of the Spanish Society for Developmental Biology,Granada,2012 (Poster).

4.	Tavares, Bárbara; Dias, Pedro; Feijó, José; Bicho, Ana. Arabidopsis thaliana and Lilium longiflorum pollen protoplasts share anionic transport characteristics,EMBO Workshop on Biophysical Mechanisms of Development,Oeiras,2011 (Poster). na.

5.

Tavares, Bárbara; Dias, Pedro; Feijó, José; Bicho, Ana. Arabidopsis thaliana and Lilium longiflorum pollen protoplasts share anionic transport characteristics,XV International Workshop on Plant Membrane Biology, Adelaide,2010 (Comunicação). The anion channels present in the plasma membrane of Arabidopsis thaliana and Lilium longiflorum pollen grain protoplasts were characterized with the whole-cell patch-clamp technique (WC), under symmetrical anionic concentrations, with chloride (Cl-) as the major permeable anion. Three outward-rectifying anionic current populations were identified from Arabidopsis thaliana protoplasts: ICl1 (current lost by rundown after entering into WC), ICl2 (current insensitive to 5-nitro-2-(phenylpropylamino)-benzoate (NPPB)), and ICl3 (current inhibited by 100 µM NPPB). ICl2 and ICl3 were measured after rundown. All 3 populations allowed the passage of current in both directions, and they were depolarization-activated and hyperpolarization-deactivated. They presented a time dependent activation, for positive membrane potentials (Vm). The Boltzmann parameters obtained from the dependence of cord conductances on Vm showed a different sensitivity of ICl1 to variations in Vm from the other two currents. The effect of 3 different intracellular [Ca2+] ([Ca2+]in, in the nM, µM and mM range), was studied showing that all 3 currents increased in intensity with increasing [Ca2+]in, while their sensitivity to NPPB decreased. The sensitivity to variations of the Vm was not changed. After rundown, the currents measured with NO3--based solutions were similar to those measured with Cl-, but PNO3/PCl = 2. Similar Cl- currents were measured in Lilium longiflorum, with different responses to [Ca2+]in, and sensitivity to NPPB. Anion channels, sharing main features, were functionally characterized, for the first time, in Arabidopsis and Lilium. These could be responsible for the observed Cl- effluxes present at the tip of growing pollen tubes.

6.

Tavares, Bárbara; Dias, Pedro; Moura, Teresa; Feijó, José; Bicho, Ana. Anionic Currents in Pollen Protoplasts from Lilium longiflorum,IV Spanish Portuguese Biophysical Congress,Zaragoza,2010 (Comunicação). The presence of anion channels in the plasma membrane of Lilium longiflorum pollen grain protoplasts was studied under symmetrical anionic concentrations by means of the whole-cell configuration of the patch-clamp technique. Three outward-rectifying anionic current populations, ICl1, ICl2 and ICl3, were identified with Cl--based intra and extracellular solutions. These shared strong outward rectification, activated instantaneously and displayed a slow time-dependent activation for most positive potentials. ICl1 was the current lost by rundown after the establishment of the whole-cell configuration. ICl2 and ICl3 are the currents measured after rundown. ICl2 was insensitive to 5-nitro-2-(phenylpropylamino)-benzoate (NPPB) while ICl3 was completely inhibited by 500 µM in the presence of nM [Ca2+]in. Additionally, the effect of three different intracellular calcium concentrations ([Ca2+]in,) in the nM, µM and mM range, was studied showing that all three currents were dependent on the [Ca2+]in. ICl1 increased intensity with increasing [Ca2+]in reaching its maximum with 0.5 mM. ICl2 and ICl3 attained maximum current and conductance values with µM [Ca2+]in. After rundown, the anionic currents measured with NO3--based solutions were indistinguishable from those measured with high Cl- concentrations, indicating that the permeability of the channels for Cl- and NO3- was of the same order of magnitude. This study shows for the first time the presence of a large anion conductance across the membrane of pollen protoplasts, due to the presence of Ca2+-regulated channels. We hypothesize that these channels may be reallocated during the onset of germination to the apical region of the growing pollen tube and, under physiological conditions when the membrane potential is expected to be negative, may be responsible for the large anionic effluxes previously reported by means of self-referencing vibrating probes.

7.

Tavares, Bárbara; Dias, Pedro; Moura, Teresa; Feijó, José; Bicho, Ana. Electrophysiological Studies on the Anion Channels from Pollen Tubes of Lilium longiflorum,1st Portuguese-Spanish-British Joint Biophysics Congress 2008,Lisboa,2008 (Poster). Pollen grains have become a foundation for basic cell biology studies involving cellular growth and morphogenesis. Upon hydration in a simple medium they germinate and develop a cytoplasmic extension called the pollen tube, which grows at impressive rates for many millimeters without dividing. The pollen tube may grow exclusively by apical exocytose, an extreme example of cellular polarity and growth. Previous studies performed by the Plant Development Group at I.G.C. have shown, by means of ion-specific vibrating probes and intracellular ion imaging, that cellular growth control in this biological system is underlined by its ion dynamics. The chloride ions present an oscillatory efflux at the tip of the pollen tube and a non oscillatory influx at the sides of the tube. Chloride currents in pollen protoplasts were measured for the first time in the membrane of protoplasts obtained from Lilium longiflorum, by means of the patch clamp technique. These are characterized by a strong outward rectification and by slow activation and deactivation mechanisms. These channels allow the entrance of chloride into the cell. It is possible that these are the channels responsible for the non oscillatory chloride influxes present in the pollen tube, but more evidence is needed to firmly establish this hypothesis. .

8.

Tavares, Bárbara; Feijó, José; Moura, Teresa; Bicho, Ana. Patch clamp on pollen´s Clc chloride channels transiently expressed in HEK293 cells,14th INTERNATIONAL WORKSHOP ON PLANT MEMBRANE BIOLOGY,Valencia,2007 (Poster). Pollen grains have become the focus of a widespread attention for basic cell biology studies involving cellular growth and morphogenesis. Upon hydration in a simple medium they germinate and develop a cytoplasmic extension called the pollen tube, which grows at impressive rates for many millimeters without dividing. The pollen tube may grow exclusively by apical exocytose (4 ¿m/sec, one of the fastest rates in nature), an extreme example of cellular polarity and growth. Previous studies performed by the Plant Development Group at I.G.C. have shown, by means of ion-specific vibrating probes and intracellular ion imaging for Ca2+, H+ and Cl-, that cellular growth control in this biological system in underlined by its ion dynamics. However, plant cell walls prevent further electrophysiological studies by invasive methods. The cloning of plant membrane transporters and its overexpression in heterologous animal systems (mammalian cell lines) amenable to patch-clamp studies, may allow the identification and characterization of the molecules involved and the understanding of the mechanisms by which they exert control on cellular growth. It has been observed that two putative chloride channels (Clc-c and Clc-d) of the Clc family are up regulated in pollen. In order to verify if the chloride fluxes observed with the vibrating probes can be explained by the currents through these chloride channels, cDNA of the two channels have been subcloned into a pcDNA with the CMV promoter for expression in mammalian cells. These clones (Clc-c and Clc-d) have been used to transiently transfect mammalian HEK293 cells, amenable for further patch-clamp characterization. Preliminary results obtained in the whole-cell configuration show an increase of the cell current that is inhibited by 1mM DPC, an established blocker for chloride currents.

9.	Tavares, Bárbara; Feijó, José; Moura, Teresa; Bicho, Ana. Patch clamp on pollen´s Clc chloride channels transiently expressed in HEK293 cells,8ª Reunião Anual do Laboratório Associado Requimte,Fátima,2006 (Poster).

10.	Tavares, Bárbara; Marques da S. J; Bernardes da S. A; Arrabaça, M. C. Water stress resistance in three golf turf grasses with different photosynthetic metabolism,VII Simposium Luso-Espanhol de Relações Hídricas Em Plantas,Faro,2004 (Comunicação).

11.	Tavares, Bárbara; Marques da S. J; Bernardes da S. A; Arrabaça, M. C. Resistance to Hydric Stress in Four Grass Species Used in Golf Courses,8º Encontro Nacional de Ecologia,Évora,2003 (Poster).

Produção científica Scientific production

Produção técnica Technical production

Total

Produção científica Scientific production

5

Artigos científicos em revistas Papers in periodics

3

Com arbitragem científica With scientific refereeing

3

Trabalhos em eventos Papers in conference proceedings

2

Com arbitragem científica With scientific refereeing

1

Sem arbitragem científica Without scientific refereeing

1

Total

Produção técnica Technical production

11

Outros tipos de produção técnica Other technical production

11

	José Alberto Bernardo de Magalhães Feijó
	Pedro Nuno Resende Dias
	Petra Alexandra Pereira Horta Rodrigues Pintado

Pedro Nuno Resende Dias