Tatiana Andreia Forjaz Amaral Catarino
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Data da última atualização
»Last update
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02/12/2013 |
Dados pessoais (Personal data)
Nome completo
Full name |
Tatiana Andreia Forjaz Amaral Catarino |
Nome em citações bibliográficas
Quoting name |
Catarino, Tatiana Andreia Forjaz Amaral |
Domínio científico de atuação
Scientific domain |
Ciências Naturais-Ciências Biológicas.
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Endereço profissional
Professional address |
Universidade de Coimbra
Centro de Neurociências de Coimbra
Faculdade de Medicina da Universidade de Coimbra pólo I Rua Larga
3004--504 Coimbra
Portugal
Correio electrónico: tatianac@cnc.uc.pt |
Sexo
Gender |
Feminino»Female |
Graus Académicos
(Academic Degrees)
| 2006-2012 |
Doutoramento
Phd |
Biologia Celular
(6 anos » years)
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Universidade de Coimbra,
Portugal.
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| 1997-2004 |
Licenciatura
Licentiate degree |
Ramo científico
(4 anos » years)
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Universidade de Coimbra,
Portugal.
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| 1997-2002 |
Licenciatura
Licentiate degree |
Ramo Educacional
(5 anos » years)
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Universidade de Coimbra,
Portugal.
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Vínculos profissionais
(Professional Positions)
| Centro de Neurociências e Biologia Celular |
| Jan/2011-Actual |
Assistente de Investigação |
| Escola Secundária de Avelar Brotero |
| Set/2001-Jun/2002 |
estagiário |
Línguas (Languages)
Compreende
Understandig |
Português (Bem), Espanhol (Bem), Inglês (Bem), Francês (Pouco), Italiano (Razoavelmente). |
Fala
Speaking |
Português (Bem), Espanhol (Razoavelmente), Inglês (Bem), Francês (Pouco), Italiano (Pouco). |
Lê
Reading |
Português (Bem), Espanhol (Bem), Inglês (Bem), Francês (Bem), Italiano (Pouco). |
Escreve
Writing |
Português (Bem), Espanhol (Pouco), Inglês (Bem), Francês (Pouco), Italiano (Pouco). |
Produção científica, técnica e artística/cultural
(Scientific, technical and artistical/cultural
production)
Artigos em revistas sem arbitragem científica
Papers in periodics without scientific refereeing |
| 1. |
 Catarino, T.; Ribeiro, L.; Santos, S. D; Carvalho, A. L. 2012. "Regulation of synapse composition by protein acetylation: the role of acetylated cortactin", Journal of Cell Science 126, 1: 149 - 162.
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Lobo, Andrea C; Gomes, João R; Catarino, Tatiana; Mele, Miranda; Fernandez, Pedro; Inácio, Ana R; Bahr, Ben A; Santos, Armanda E; Wieloch, Tadeusz; Carvalho, Ana L; Duarte, Carlos B. 2011. "Cleavage of the vesicular glutamate transporters under excitotoxic conditions", Neurobiology of Disease 44, 3: 292 - 303.
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Dados Complementares (Additional data)
Participação em eventos
Event participation |
Outro tipo de participação
Other kind of participation |
| 1. |
Cortactin acetylation regulates the maturation of excitatory synapses, 2011 (Congresso).
Nome do evento: 12th Meeting of the Portuguese Society for Neuroscience. |
| 2. |
Cortactin acetylation regulates synapse maturation, 2010 (Congresso).
Nome do evento: The Synapse Symposium. |
| 3. |
Cortactin acetylation regulates synapse maturation, 2010 (Congresso).
Nome do evento: 7th FENS Forum of European Neuroscience; Cidade do evento: Amesterdão / Holanda. |
| 4. |
Cortactin acetylation regulates synapse maturation, 2010 (Congresso).
Nome do evento: 8th Center for Neuroscience Annual Meeting; Nome da Instituição: Centro de Neurociâncias e Biologia Celular; Cidade do evento: Coimbra / Portugal. |
| 5. |
Trafficking of AMPA receptors: Unveiling the role of cortactin, 2007 (Congresso).
Nome do evento: 10th Meeting of the Portuguese Society for Neuroscience. |
| 6. |
Trafficking of AMPA receptors: Unveiling the role of cortactin, 2007 (Congresso).
Nome do evento: 5th Center for Neuroscience Annual Meeting. |
| 7. |
Role of Cortactin in the trafficking of AMPA-Type Glutamate receptors, 2006 (Congresso).
Nome do evento: Synapse Summer School. |
| 8. |
Role of Cortactin in the trafficking of AMPA-Type Glutamate receptors, 2006 (Congresso).
Nome do evento: 4th Center for Neuroscience Annual Meeting. |
Indicadores de produção
(Production indicators)
Outras informações relevantes
Área de actividade científica - Glutamatergic Synapses - Formation and regulation of synapses Neurons have a complex morphology,
with branched dendrites exhibiting thousands of synapses, a specialized region where communication between neurons occurs.
We are interested in understanding how these connections between nerve cells are formed and how they are modified with experience.
The ability of synapses to change their strength is thought to be the cellular correlate of learning and memory, and synaptic
dysfunction is correlated with several neurodegenerative diseases.
We focus on excitatory glutamatergic synapses, and
study their regulation and formation from a cellular and molecular biology viewpoint.
We use a combination of techniques
like primary neuronal cultures, biochemistry, molecular and cellular biology to address these questions.
Technical
skills and competences Cell Biology Hippocampal cells primary cultures; HEK-293 cell line; Transformation of bacteria cells;
Plasmid transfections in primary cultures using the calcium phosphate method; Plasmid transfections in cell lines using both
the calcium phosphate method and lipofectamine; SDS-polyacrilamide gel electrophoresis; Western blotting; Protein concentration
determination using the BCA method; Polyacrilamyde gel staining with Coomassie blue; PVDF membrane staining with Coomassie
blue; Pull-down and immunoprecipitation assays; Immunocytochemistry; Fluorescence microscopy Molecular Biology PCR and
RT-PCR; Plasmid cloning, in situ mutagenesis and deletions, RNA extraction and purification and DNA purification using kits
for mini, midi and maxi prep and homemade solutions; Recombinant GST fusion protein and His-tagged protein expression and
purification from E.
Coli; DNA analysis and purification from agarose gel using kits; Preparation of E.
coli competent
cells Computer skills and competences: Experience in laboratory programs such as GraphPad Prism (statistical analysis),
Endnote (References database), ImageQuant (western blot quantification), Vector NTI (Molecular Biology program), AxioVision
(Microscope software) and ImageJ.
Experience also with Microsoft Word, Power Point and Excel.
Complementary training
and formation: 2008, 3-6 June - "Gene Silencing using RNA Interference", International Postgraduated Programme, University
of Minho, Braga, Portugal 2006, 9-30 September - PENS Training Center, Synapse Summer School.
Bordeaux, France 2004,
15-19 November – Doctoral Programme in Experimental Biology and Biomedicine – “Molecular Neuroscience course” organized by
Center for Neuroscience and Cell Biology (CNC) - University of Coimbra, Portugal
Actuais interesses de investigação -Role of cortactin in synapse maturation
Focus on excitatory glutamatergic synapses, and study their regulation and formation from a cellular and molecular biology
viewpoint.
The number of dendritic spines, where excitatory synapses are located, is regulated by molecules that organiz
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