Ana Rita Pinto Seabra
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Data da última atualização
»Last update
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14/04/2016 |
Dados pessoais (Personal data)
Nome completo
Full name |
Ana Rita Pinto Seabra |
Nome em citações bibliográficas
Quoting name |
Seabra, Ana Rita |
Categoria profissional
Position |
Investigador Auxiliar |
Domínio científico de atuação
Scientific domain |
Ciências Naturais-Ciências Biológicas.
Ciências Agrárias-Outras Ciências Agrárias.
Ciências Médicas-Ciências da Saúde.
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Sexo
Gender |
Feminino»Female |
Graus Académicos
(Academic Degrees)
| 2007-2012 |
Doutoramento
Phd |
Doutoramento em Biologia
(5 anos » years)
.
Faculdade de Ciências da Universidade do Porto,
Portugal.
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| 2000-2004 |
Licenciatura
Licentiate degree |
Bioquímica - ramo bioquímica aplicada
(4 anos » years)
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Faculdade de Ciências da Universidade do Porto,
Portugal.
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Formação complementar ( studies)
| 2013 |
Outros
Others |
Project Management for Post-Docs.
Max-Planck-institut für Pflanzenzüchtungsforschung,
Alemanha.
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| 2011 |
Outros
Others |
3rd UTEN training week - From the Lab to the market.
Universidade do Algarve,
Portugal.
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Vínculos profissionais
(Professional Positions)
| Instituto de Biologia Molecular e Celular |
| Fev/2015-Actual |
Investigador Auxiliar |
| Fev/2014-Dez/2014 |
Investigador Auxiliar |
| Mar/2012-Mai/2012 |
Investigador Auxiliar |
| Jul/2011-Fev/2012 |
Investigador Auxiliar |
| Jul/2007-Jun/2011 |
Outra Situação |
| Nov/2004-Jun/2007 |
Assistente de Investigação |
| Max-Planck-institut für Pflanzenzüchtungsforschung |
| Jun/2012-Jan/2014 |
Investigador Auxiliar |
| Nov/2011-Abr/2012 |
Outra Situação |
| Instituto de Ciências Biomédicas Abel Salazar |
| Out/2003-Out/2004 |
Estagiário Investigador |
Atividades de Investigação e Desenvolvimento (Research and Development activities)
| Max-Planck-institut für Pflanzenzüchtungsforschung |
| Instituto de Biologia Molecular e Celular |
Estágios realizados (Traineeships)
Estágios realizados»Traineeships:
- UPINtech programme
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| Instituto de Ciências Biomédicas Abel Salazar |
Estágios realizados»Traineeships:
- Estudo de mecanismos imunológicos de resistência ou susceptibilidade à infecção experimental por Neospora caninum em murganhos.
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Linhas de Investigação (Research fields)
| 1. |
Role and regulation of Glutamine synthetase (GS) in Medicago truncatula |
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Objectivos»Goals:
(i) Characterize the entire GS gene family in Medicago truncatula (ii) Analyze patterns of gene and protein expression (iii)
Determine the catalytic properties of each isoenzyme.
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| 2. |
Transcript expression profilling of root nodules with altered levels of GS activity |
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Objectivos»Goals:
(i) Transcriptome analysis of root nodules with inactive GS (ii) Identification of genes associated with GS activity .
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| 3. |
Post-translational regulation of Glutamine Synthetase (GS) by ubiquitin and/or ubiquitin-like proteins |
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Objectivos»Goals:
(i) Characterize the putative post-translational regulation of GS by ubiquitin and/or ubiquitin like proteins (ii) investigate
the functional relevance of these modifications for nitrogen assimilation in Medicago truncatula.
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| 4. |
Nitrogen signaling and plant development in Medicago truncatula |
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Objectivos»Goals:
It is well known that microbes are able to modulate plant development. An important example is the interaction of legumes
with nitrogen-fixing bacteria (Rhizobium), which leads to the development of a specialized organ where nitrogen fixation takes
place – the root nodule. Nodule organogenesis is a process that occurs only under nitrogen-limiting conditions and that is
promptly terminated when an inorganic source of nitrogen is sensed. Hence, components of nodulation pathways must surely overlap
that of the nitrogen sensing/signaling pathways. Although some of the key players within nitrogen signaling pathways have
been revealed in recent years, we are still far from completely understanding this complex network. I am currently interested
in investigating how nitrogen signals are integrated with plant developmental processes, at the molecular level, particularly
during the Legume-Rhizobium interaction. Within this context I aim to understand the role and regulation of prokaryotic GSI-like
proteins in M. truncatula..
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| 5. |
Plant-Obligate biotrophs interactions |
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Objectivos»Goals:
A key question that has been poorly addressed is how plant obligate parasites adapt to new hosts? Recent findings lead us
to hypothesize that heat shock protein 90 (HSP90) may be involved in such process. For instance, during stress HSP90 appears
to link pre-acquired genetic diversity with fast appearance of new traits in eukaryotes. In addition, HSP90 appears to function
as a virulence factor in human protozoa parasites.
The goal of this research was to investigate the impact of cytoplasmic, and especially apoplastic HSP90s, on adaptation of
obligate parasites to new plant hosts, to be specific, to investigate whether the proteins promote virulence and/or function
as a buffer for genetic variation. To explore this topic I used the model plant Arabidopsis thaliana and the obligate parasitic
oomycete Albugo laibachii. To reach my objective, I addressed the following questions: 1) How are HSP90s secreted? 2) What
is the relative contribution of cytoplasmic versus apoplastic HSP90 to virulence? 3) What is the impact of stress, and hence
the impact of HSPs to host shifts?
.
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Projetos de Investigação (Research projects)
Participação como Investigador
Participation as Researcher |
2013-
Regulação da Glutamina sintetase pelo Oxido Nitrico em Medicago truncatula.-Regulation of Glutamine synthetase by Nitric Oxide in Medicago truncatula |
| Referência do projeto»Project reference: PTDC/BIA-PLA/2291/2012.
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Participação como Doutorando
Participation as Phd student |
2010-2013
Estudo da função e regulação da glutamina sintetase em sementes de Medicago truncatula-Study of the role and regulation of glutamine synthetase in Medicago truncatula seeds |
| Referência do projeto»Project reference: PTDC/AGR-AAM/099577/2008.
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Participação como Desenvolvimento técnico
Participation as Technical developer |
2005-2009
Determinação da estrutura tri-dimensional da glutamina sintetase de Medicago truncatula.-Elucidation of the three-dimensional structure of glutamine synthetase from the model legume Medicago truncatula |
| Referência do projeto»Project reference: POCI/AGR/61025/2004.
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2004-2007
Estudo da contribuição da glutamina sintetase para a eficiência de assimilação do azoto e produtividade vegetal em M. truncatula-Analysis of the contribution of root nodule glutamine synthetase in controlling nitrogen assimilation efficiency and biomass
production in M. truncatula
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| Referência do projeto»Project reference: POCTI/AGG/39079/2001.
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Línguas (Languages)
Compreende
Understandig |
Português (Bem), Inglês (Bem), Francês (Razoavelmente), Espanhol (Razoavelmente), Alemão (Razoavelmente). |
Fala
Speaking |
Português (Bem), Inglês (Bem), Espanhol (Razoavelmente). |
Lê
Reading |
Português (Bem), Inglês (Bem), Francês (Razoavelmente), Espanhol (Razoavelmente), Alemão (Razoavelmente). |
Escreve
Writing |
Português (Bem), Inglês (Bem). |
Membro de Associações Profissionais/Científicas (Professional/Scientific Association membership)
| Jan/2012 - Actual |
Sociedade Portuguesa de Bioquímica, Membro.
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Produção científica, técnica e artística/cultural
(Scientific, technical and artistical/cultural
production)
Artigos em revistas com arbitragem científica
Papers in periodics with scientific refereeing |
| 1. |
Seabra, Ana R; Carvalho, Helena G. 2015. "Glutamine synthetase in Medicago truncatula, unveiling new secrets of a very old enzyme", Frontiers in Plant Science 6, 578: 1 - 7.
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| 2. |
 Silva, Liliana S; Seabra, Ana R; Leitão, José N; Carvalho, Helena G. 2015. "Possible role of glutamine synthetase of the prokaryotic type (GSI-like) in nitrogen signaling in Medicago truncatula", Plant Science, 240: 98 - 108.
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| 3. |
Torreira, Eva; Seabra, Ana R; Marriott, Hazel; Zhou, Min; Llorca, Óscar; Robinson, Carol V; Carvalho, Helena G; Fernández-Tornero, Carlos; Pereira, Pedro J. B. 2014. " The structures of cytosolic and plastid-located glutamine synthetases from Medicago truncatula
reveal a common and dynamic architecture ", Acta Crystallographica Section D Biological Crystallography 70, 4: 981 - 993.
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| 4. |
Seabra, Ana R; Silva, Liliana S; Carvalho, Helena G. 2013. "Novel aspects of glutamine synthetase (GS) regulation revealed by a detailed expression analysis of the entire GS gene family
of Medicago truncatula under different physiological conditions", BMC Plant Biology 13, 1: 137 - 137.
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| 5. |
Seabra, Ana R; Pereira, Patrícia A; Becker, Jörg D; Carvalho, Helena G. 2012. " Inhibition of Glutamine Synthetase by Phosphinothricin Leads to Transcriptome Reprograming in Root Nodules of
Medicago truncatula ", Molecular Plant-Microbe Interactions 25, 7: 976 - 992.
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| 6. |
Melo, P. M; Silva, L. S; Ribeiro, I.; Seabra, A. R; Carvalho, H. G. 2011. "Glutamine Synthetase Is a Molecular Target of Nitric Oxide in Root Nodules of Medicago truncatula and Is Regulated by Tyrosine
Nitration", PLANT PHYSIOLOGY 157, 3: 1505 - 1517.
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| 7. |
AR, Seabra; CP, Vieira; JV, Cullimore; HG, Carvalho. 2010. "Medicago truncatula contains a second gene encoding a plastid located glutamine synthetase exclusively expressed in developing
seeds.", BMC plant biology 10, 1: 183 - 183.
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| 8. |
Seabra, Ana R; Carvalho, Helena; Pereira, Pedro J. B. 2009. " Crystallization and preliminary crystallographic characterization of glutamine synthetase from
Medicago truncatula ", Acta Crystallographica Section F Structural Biology and Crystallization Communications 65, 12: 1309 - 1312.
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| 9. |
Lima, L.; Seabra, Ana; Melo, Paula; Cullimore, Julie; Carvalho, Helena. 2006. "Post-translational regulation of cytosolic glutamine synthetase of Medicago truncatula", Journal of Experimental Botany 57, 11: 2751 - 2761.
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| 10. |
Lima, Lígia; Seabra, Ana; Melo, Paula; Cullimore, Julie; Carvalho, Helena. 2005. "Phosphorylation and subsequent interaction with 14-3-3 proteins regulate plastid glutamine synthetase in Medicago truncatula", Planta 223, 3: 558 - 567.
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| 11. |
Teixeira, Luzia; Marques, Andreia; Meireles, Carla S; Seabra, Ana R; Rodrigues, Diana; Madureira, Pedro; Faustino, Augusto M. R; Silva, Carolina; Ribeiro, Adilia; Ferreira, Paula; Correia da C. J. M; Canada, Nuno; Vilanova, Manuel. 2005. "Characterization of the B-cell immune response elicited in BALB/c mice challenged with Neospora caninum tachyzoites", Immunology 116, 1: 38 - 52.
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Apresentação oral de trabalho
Oral work presentation |
| 1. |
LS, Silva; Seabra, Ana R. P; HG, Carvalho. Expression of GSI-like genes in root nodules of Medicago truncatula. ,10th European Nitrogen Fixation Conference,Munich,2012 (Poster). |
| 2. |
Seabra, Ana R. P; PA, Pereira; JD, Becker; HG, Carvalho. Inhibition of glutamine synthetase by phosphinothricin leads to transcriptome reprogramming in Medicago truncatula root nodules.
,Meeting on Plant-Microbe Interactions,Braga,2011 (Comunicação). |
| 3. |
Seabra, Ana R. P; PA, Pereira; JD, Becker; HG, Carvalho. Inhibition of glutamine synthetase by phosphinothricin leads to transcriptome reprogramming in Medicago truncatula root nodules.,Model Legume congress 2011,Sainte-Maxime,2011 (Poster). |
| 4. |
PM, Melo; LS, Silva; Seabra, Ana R. P; HG, Carvalho. Regulation of glutamine synthetase by reactive nitrogen species in root nodules of Medicago truncatula.,10th International Conference on Reactive Oxygen and Nitrogen Species in Plants,Budapest,2011 (Comunicação). |
| 5. |
PM, Melo; Seabra, Ana R. P; LS, Silva; HG, Carvalho. Reversible inactivation of glutamine synthetase by reactive nitrogen species in root nodules of Medicago truncatula., 9th European Nitrogen Fixation Conference,Geneve,2010 (Comunicação). |
| 6. |
Seabra, Ana R. P; CP, Vieira; JV, Cullimore; HG, Carvalho. Characterization of a gene duplication encoding a plastid located glutamine synthetase exclusively expressed in developing
seeds of Medicago truncatula. ,Vth International Congress on Legume Genetics and Genomics,Pacific grove,2010 (Comunicação). |
| 7. |
PM, Melo; Seabra, Ana R. P; LS, Silva; HG, Carvalho. Regulation of glutamine synthetase by reactive nitrogen species in root nodules of Medicago truncatula.,XVII Congresso Nacional de Bioquímica,Porto,2010 (Poster). |
| 8. |
PA, Pereira; Seabra, Ana R. P; N, Hohnjec; R, Balestrini; E, Limpens; P, Bonfante; H, Kuster; HG, Carvalho; JD, Becker. The Symbiotic Genetic Programme of the Model Legume Medicago truncatula., XXXV Portuguese Genetics Conference,Braga,2010 (Poster). |
| 9. |
Seabra, Ana R. P; PA, Pereira; JD, Becker; HG, Carvalho. Inhibition of glutamine synthetase by phosphinothricin leads to transcriptome reprogramming in Medicago truncatula root nodules.
,XVII Congresso Nacional de Bioquímica,Porto,2010 (Poster). |
| 10. |
Seabra, Ana R. P; PA, Pereira; JD, Becker; HG, Carvalho. Transcriptome analysis of Medicago truncatula root nodules following inhibition of glutamine synthetase by phosphinothricin,9th European Nitrogen Fixation Conference September,Geneve,2010 (Poster). |
| 11. |
LS, Silva; Seabra, Ana R. P; PM, Melo; HG, Carvalho. Characterization of a glutamine synthetase phosphorylating kinase isolated from root nodules of Medicago truncatula.,9th European Nitrogen Fixation Conference September,Geneve,2010 (Poster). |
| 12. |
LS, Silva; Seabra, Ana R. P; PM, Melo; HG, Carvalho. Identification of a glutamine synthetase putative phosphorylating kinase from Medicago truncatula.,XVII Congresso Nacional de Bioquímica,Porto,2010 (Poster). |
| 13. |
I, Ribeiro; Seabra, Ana R. P; HG, Carvalho; PM, Melo. In vitro inactivation of plant glutamine synthetase by tyrosine nitration.,XVI Congress of the Federation of European Societies of Plant Biology (FESPB),Tampere,2008 (Poster). |
| 14. |
Seabra, Ana R. P; C, Fernández-Tornero; G, Schoehn; PJ, Pereira; HG, Carvalho. Structural features of glutamine synthetase from Medicago truncatula.,XVI Congress of the Federation of European Societies of Plant Biology (FESPB),Tampere,2008 (Poster). |
| 15. |
I, Ribeiro; PM, Melo; Seabra, Ana R. P; HG, Carvalho. In vitro inactivation of plant glutamine synthetase by tyrosine nitration.,16º Congresso Nacional de Bioquímica,Açores,2008 (Poster). |
| 16. |
Seabra, Ana R. P; JV, Cullimore; HG, Carvalho. Overexpression of glutamine synthetase in root nodules of Medicago truncatula leads to enhanced nitrogen utilization efficiency.,XVI Congress of the Federation of European Societies of Plant Biology (FESPB),Tampere,2008 (Poster). |
| 17. |
Seabra, Ana R. P; HG, Carvalho. Simultaneous 15N/13C labelling indicates that rhizobial nitrogen fixation is influenced by the levels of glutamine synthetase
in root nodules.,16º Congresso Nacional de Bioquímica,Açores,2008 (Poster). |
| 18. |
Seabra, Ana R. P; C, Fernández-Tornero; G, Schoehn; PJ, Pereira; HG, Carvalho. Decameric organization of glutamine synthetase from Medicago truncatula.,Nitrogen 2007-International Symposium on Inorganic Nitrogen Assimilation,Lancaster,2007 (Poster). |
| 19. |
Seabra, Ana R. P; P, Thoquet; JV, Cullimore; HG, Carvalho. Assessment of the role of glutamine synthetase in the control of nitrogen metabolism in root nodules., 6th European Conference on Grain legume,Lisboa,2007 (Poster). |
| 20. |
Seabra, Ana R. P; P, Thoquet; JV, Cullimore; HG, Carvalho. Analysis of transgenic Medicago truncatula overexpressing glutamine synthetase in root nodules: phenotypic characterization
and transcript profiling.,Nitrogen 2007-International Symposium on Inorganic Nitrogen Assimilation,Lancaster,2007 (Poster). |
| 21. |
Seabra, Ana R. P; HG, Carvalho; PJ, Pereira. Three-dimensional structure of an eukaryotic glutamine synthetase.,15º Congresso Nacional de Bioquímica,Aveiro,2006 (Poster). |
| 22. |
Seabra, Ana R. P; HG, Carvalho. Silencing plastid located glutamine synthetase through RNAi in root nodules of transgenic Medicago truncatula.,15º Congresso Nacional de Bioquímica,Aveiro,2006 (Poster). |
| 23. |
Teixeira, Luzia; Seabra, Ana R. P; Rodrigues, Diana; Ribeiro, Adilia; Vilanova, Manuel. Increased susceptibility to nosporosis in mice immunized with Neospora caninum tachyzoites structural antigens,XXX reunião anual da sociedade portuguesa de imunologia,Lisboa,2005 (Poster). |
| 24. |
L, Lima; Seabra, Ana R. P; PM, Melo; HG, Carvalho. Identification of serine 97 as a major regulatory phosphorylation site in plastid glutamine synthetase from Medicago truncatula., IX Congresso Luso-Espanhol de Fisiologia Vegetal,Évora,2005 (Poster). |
| 25. |
L, Lima; Seabra, Ana R. P; PM, Melo; HG, Carvalho. 14-3-3 binding controls proteolysis of plastid located glutamine synthetase in Medicago truncatula., IX Congresso Luso-Espanhol de Fisiologia Vegetal,Évora,2005 (Poster). |
| 26. |
Teixeira, Luzia; Rodrigues, Diana; Seabra, Ana R. P; Ribeiro, Adilia; Vilanova, Manuel. Characterization of the B-cell immune response elicited in BALB/c mice challenged with Neospora caninum tachyzoites.,XXX reunião anual da sociedade portuguesa de imunologia,Lisboa,2005 (Poster). |
Curso de curta duração lecionado
Taught short course |
| 1. |
Seabra, Ana R. P; Carvalho, Helena. Highlights of plant engineering, 2014 (Extensão), promovido por II AEICBAS Biomedical Congress: an innovative multidisciplinary approach of life sciences.
Duração: 1 dias. Local: ICBAS, Cidade: Porto, Tipo de participação: Docente. |
| 2. |
Seabra, Ana R. P; Figueiredo, Raquel. Porto de crianças - actividades de coadjuvação curricular, 2011 (Extensão), promovido por Município do Porto e IBMC.
Duração: 4 dias. Local: Escola básica da Pasteleira, Cidade: Porto, Tipo de participação: Docente. |
| 3. |
Seabra, Ana R. P; Figueiredo, Raquel. Porto de crianças - Actividades de coadjuvação curricular, 2010 (Extensão), promovido por Município do Porto e IBMC.
Duração: 4 dias. Local: Escola básica Augusto Bessa, Cidade: Porto, Tipo de participação: Docente. |
Outra produção artística/cultural
Other artistical/cultural production |
| 1. |
Alexandre, Filipa; Barros, Ana; Seabra, Ana R. P; Nunes, Anabela; Silva, Andreia; Marinho, Joana; Correia, Maria R; Pereira, Marisa; Ferreira, Raquel; Moutinho, Tatiana. A origem das quase espécies - teatro de marionetas de mandrágora,2009. |
Dados Complementares (Additional data)
Participação no júri de Graus Académicos
Academic Degrees jury participation |
Bacharelato/Licenciatura
Degree of licentiate |
| 1. |
Seabra, Ana Rita. Participação no júri de Francisca Isabel Alves da Silva Carvalhal. Caracterização molecular dos S2-, S5-, S10- e S25-haplótipos dos genes SFBB2, SFBB5, SFBB6, SFBB14 e SFBB16 em Malus, 2015. Trabalho de Conclusão de Curso (Bacharelato/Licenciatura em Bioquímica - ramo bioquímica aplicada) - Faculdade de Ciências da Universidade do Porto. |
| 2. |
Seabra, Ana Rita Pinto. Participação no júri de Christina Casal Ribeiro Branco. Molecular characterization of the SFBB genes in Malus S2-, S5-, S10-, and S25-haplotypes, 2014. Trabalho de Conclusão de Curso (Bacharelato/Licenciatura em Licenciatura) - Faculdade de Ciências da Universidade do Porto. |
Participação em eventos
Event participation |
Participação como Membro da Comissão Científica
Participation as Member of the Program Committee |
| 1. |
1st “Triangle Meeting” between the Max Planck Institute for Plant Breeding Research in Cologne, the Sainsbury Laboratory University
of Cambridge and the groups of Detlef Weigel and Gerd Jürgens from the Max Planck Institute for Developmental Biology in Tübingen.
Cologne, Germany, 2013 (Encontro).
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| 2. |
Careers in Science: Life after a PhD, 2010 (Simpósio).
Nome do evento: Careers in Science: Life after a PhD. |
Indicadores de produção
(Production indicators)
Outras informações relevantes
My research expertise relates mainly to nitrogen metabolism in legumes with emphasis on nitrogen assimilation in root nodules
and developing seeds.
Most of my work has focus on clarifying aspects related to the role and regulation of glutamine
synthetase isoenzymes in Medicago truncatula.
To accomplish my goals I have combined different disciplines such as physiology,
molecular biology, biochemistry and structural biology and have successfully published my work in scientific journals of high
impact.
During my research career I have also engaged on different projects related to host-microbe interactions, which
have broadened my research skills in ecology, microbiology and immunology.
As a post-doc researcher I used the Arabidopsis
thaliana-Albugo laibachii pathosystem to understand the life style of plant biotroph pathogens and as an undergraduate intern
I studied the B-cell response of mice models to the parasite Neospora caninum.
Other interests include the development of sustainable and effective methods of crop production and actions of science communication
for general public, which included participation as a teacher in children educational activities promoted by Porto Municipality.
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